ISY1_RAT
ID ISY1_RAT Reviewed; 284 AA.
AC Q6AYB3;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Pre-mRNA-splicing factor ISY1 homolog;
GN Name=Isy1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the spliceosome C complex required for the
CC selective processing of microRNAs during embryonic stem cell
CC differentiation (By similarity). Required for the biogenesis of all
CC miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By
CC similarity). Only required for the biogenesis of miR-290 and miR-96
CC from the pri-miR-290-295 and pri-miR-96-183 primary transcripts,
CC respectively (By similarity). Required during the transition of
CC embryonic stem cells (ESCs) from the naive to primed state (By
CC similarity). By enhancing miRNA biogenesis, promotes exit of ESCs from
CC the naive state to an intermediate state of poised pluripotency, which
CC precedes transition to the primed state (By similarity). Involved in
CC pre-mRNA splicing as component of the spliceosome.
CC {ECO:0000250|UniProtKB:Q69ZQ2, ECO:0000250|UniProtKB:Q9ULR0}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. Component of the XAB2
CC complex, a multimeric protein complex composed of XAB2, PRPF19, AQR,
CC ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB)
CC complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is
CC incorporated into the spliceosome as a preassembled complex. The IB
CC complex does not contain PRPF19. Interacts with CPSF3; this interaction
CC is in an RNA independent manner (By similarity). Interacts with the
CC microprocessor complex subunits DGCR8 and DROSHA; this interaction is
CC in an RNA dependent manner (By similarity).
CC {ECO:0000250|UniProtKB:Q69ZQ2, ECO:0000250|UniProtKB:Q9ULR0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9ULR0}.
CC -!- SIMILARITY: Belongs to the ISY1 family. {ECO:0000305}.
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DR EMBL; BC079119; AAH79119.1; -; mRNA.
DR RefSeq; NP_001014210.1; NM_001014188.1.
DR AlphaFoldDB; Q6AYB3; -.
DR SMR; Q6AYB3; -.
DR IntAct; Q6AYB3; 1.
DR STRING; 10116.ENSRNOP00000013571; -.
DR iPTMnet; Q6AYB3; -.
DR PhosphoSitePlus; Q6AYB3; -.
DR PaxDb; Q6AYB3; -.
DR PRIDE; Q6AYB3; -.
DR Ensembl; ENSRNOT00000013571; ENSRNOP00000013571; ENSRNOG00000010021.
DR GeneID; 362394; -.
DR KEGG; rno:362394; -.
DR UCSC; RGD:1307688; rat.
DR CTD; 57461; -.
DR RGD; 1307688; Isy1.
DR eggNOG; KOG3068; Eukaryota.
DR GeneTree; ENSGT00390000014109; -.
DR HOGENOM; CLU_043453_0_0_1; -.
DR InParanoid; Q6AYB3; -.
DR OMA; IPSCEKW; -.
DR OrthoDB; 1513531at2759; -.
DR PhylomeDB; Q6AYB3; -.
DR TreeFam; TF105841; -.
DR Reactome; R-RNO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-RNO-6782135; Dual incision in TC-NER.
DR Reactome; R-RNO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-RNO-72163; mRNA Splicing - Major Pathway.
DR PRO; PR:Q6AYB3; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000010021; Expressed in thymus and 19 other tissues.
DR Genevisible; Q6AYB3; RN.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:RGD.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR GO; GO:0071020; C:post-spliceosomal complex; IBA:GO_Central.
DR GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISS:UniProtKB.
DR GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR GO; GO:0000389; P:mRNA 3'-splice site recognition; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR Gene3D; 1.10.287.660; -; 1.
DR InterPro; IPR029012; Helix_hairpin_bin_sf.
DR InterPro; IPR009360; Isy1.
DR InterPro; IPR037200; Isy1_sf.
DR PANTHER; PTHR13021; PTHR13021; 1.
DR Pfam; PF06246; Isy1; 1.
DR SUPFAM; SSF140102; SSF140102; 1.
PE 2: Evidence at transcript level;
KW Acetylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Spliceosome.
FT CHAIN 1..284
FT /note="Pre-mRNA-splicing factor ISY1 homolog"
FT /id="PRO_0000235815"
FT REGION 196..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..227
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 127
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULR0"
FT MOD_RES 246
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULR0"
SQ SEQUENCE 284 AA; 32861 MW; 500C67C02C9AB1E9 CRC64;
MARNAEKAMT ALARFRQAQL EEGKVKERRP FLASECTELP KAEKWRRQII GEISKKVAQI
QNAGLGEFRI RDLNDEINKL LREKGHWEVR IKELGGPDYG KVGPKMLDHE GKEVPGNRGY
KYFGAAKDLP GVRELFEKEP LPPPRKTRAE LMKAIDFEYY GYLDEDDGVI VPLEQEYEKR
LRAELVEKWK AEREARLARG EKEEEEEEEE INIYAVTEEE SDEEGSQEKA GEDGQQKFIA
HVPVPSQQEI EEALVRRKKM ELLQKYASET LQAQSEEAKR LLGY
