ID   ISY1_SCHPO              Reviewed;         217 AA.
AC   O74370;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Pre-mRNA-splicing factor cwf12;
DE   AltName: Full=Complexed with cdc5 protein 12;
GN   Name=cwf12; Synonyms=isy1; ORFNames=SPBC32F12.05c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA   Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT   "Proteomics analysis reveals stable multiprotein complexes in both fission
RT   and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT   splicing factors, and snRNAs.";
RL   Mol. Cell. Biol. 22:2011-2024(2002).
CC   -!- FUNCTION: Involved in mRNA splicing and cell cycle control.
CC   -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC       spliceosome sub-complex reminiscent of a late-stage spliceosome
CC       composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC       cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC       cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC       cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC       cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC       prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC       smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC   -!- INTERACTION:
CC       O74370; P39964: cdc5; NbExp=3; IntAct=EBI-538882, EBI-538771;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ISY1 family. {ECO:0000305}.
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DR   EMBL; CU329671; CAA19366.1; -; Genomic_DNA.
DR   PIR; T40229; T40229.
DR   RefSeq; NP_596148.1; NM_001022067.2.
DR   AlphaFoldDB; O74370; -.
DR   SMR; O74370; -.
DR   BioGRID; 276762; 26.
DR   IntAct; O74370; 4.
DR   STRING; 4896.SPBC32F12.05c.1; -.
DR   iPTMnet; O74370; -.
DR   MaxQB; O74370; -.
DR   PaxDb; O74370; -.
DR   PRIDE; O74370; -.
DR   EnsemblFungi; SPBC32F12.05c.1; SPBC32F12.05c.1:pep; SPBC32F12.05c.
DR   GeneID; 2540230; -.
DR   KEGG; spo:SPBC32F12.05c; -.
DR   PomBase; SPBC32F12.05c; cwf12.
DR   VEuPathDB; FungiDB:SPBC32F12.05c; -.
DR   eggNOG; KOG3068; Eukaryota.
DR   HOGENOM; CLU_043453_2_0_1; -.
DR   InParanoid; O74370; -.
DR   OMA; IPSCEKW; -.
DR   PhylomeDB; O74370; -.
DR   Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR   Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   PRO; PR:O74370; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IDA:PomBase.
DR   GO; GO:0071020; C:post-spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0000974; C:Prp19 complex; IDA:PomBase.
DR   GO; GO:0005681; C:spliceosomal complex; IDA:PomBase.
DR   GO; GO:0071008; C:U2-type post-mRNA release spliceosomal complex; IDA:PomBase.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR   GO; GO:0000389; P:mRNA 3'-splice site recognition; IBA:GO_Central.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; ISS:PomBase.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   InterPro; IPR009360; Isy1.
DR   InterPro; IPR037200; Isy1_sf.
DR   PANTHER; PTHR13021; PTHR13021; 1.
DR   Pfam; PF06246; Isy1; 1.
DR   SUPFAM; SSF140102; SSF140102; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..217
FT                   /note="Pre-mRNA-splicing factor cwf12"
FT                   /id="PRO_0000192973"
SQ   SEQUENCE   217 AA;  25633 MW;  6E829D95858F3A85 CRC64;
     MSSAKYVVGS KPTEKRPKDI KSIKSVPICE KHRASVVKDI SRKISRIQSA TLPEYQIRDL
     NDAINRLMRE KHEWEVQIRD LGGINYLYNK AKLFEDEGEQ ISDIDDYRYY GRARELPGVK
     ELFEADMSFI PERQRKQEMQ KRRLDAWYFG YIPPAQESLL EDFEAKIEEQ QHKHLENLGD
     EVEQDWKPLV IEQIPTRDDV EAILLERRKN ALLSRIS