ID   ISY1_USTMA              Reviewed;         351 AA.
AC   Q4PEZ0; A0A0D1EAL2;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Pre-mRNA-splicing factor ISY1;
GN   Name=ISY1; ORFNames=UMAG_01323;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in pre-mRNA splicing. {ECO:0000250}.
CC   -!- SUBUNIT: Associated with the spliceosome. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ISY1 family. {ECO:0000305}.
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DR   EMBL; CM003141; KIS71425.1; -; Genomic_DNA.
DR   RefSeq; XP_011387232.1; XM_011388930.1.
DR   AlphaFoldDB; Q4PEZ0; -.
DR   SMR; Q4PEZ0; -.
DR   STRING; 5270.UM01323P0; -.
DR   EnsemblFungi; KIS71425; KIS71425; UMAG_01323.
DR   GeneID; 23562388; -.
DR   KEGG; uma:UMAG_01323; -.
DR   VEuPathDB; FungiDB:UMAG_01323; -.
DR   eggNOG; KOG3068; Eukaryota.
DR   HOGENOM; CLU_043453_0_1_1; -.
DR   InParanoid; Q4PEZ0; -.
DR   OMA; HHWENQI; -.
DR   OrthoDB; 1513531at2759; -.
DR   Proteomes; UP000000561; Chromosome 2.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0071014; C:post-mRNA release spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0071020; C:post-spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IBA:GO_Central.
DR   GO; GO:0000389; P:mRNA 3'-splice site recognition; IBA:GO_Central.
DR   Gene3D; 1.10.287.660; -; 1.
DR   InterPro; IPR029012; Helix_hairpin_bin_sf.
DR   InterPro; IPR009360; Isy1.
DR   InterPro; IPR037200; Isy1_sf.
DR   PANTHER; PTHR13021; PTHR13021; 1.
DR   Pfam; PF06246; Isy1; 1.
DR   SUPFAM; SSF140102; SSF140102; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Spliceosome.
FT   CHAIN           1..351
FT                   /note="Pre-mRNA-splicing factor ISY1"
FT                   /id="PRO_0000192974"
FT   REGION          202..241
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          254..327
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        202..224
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        274..292
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   351 AA;  39871 MW;  C37089B2A2A56874 CRC64;
     MARNQEKAQS MLYRFREAQA TSLGVSTKPA RRPRLASSVS SLKECERWRS DVIREISRKV
     SKIQDFGLND YEVRDLNDEI NKLLREKGHW ENQIVALGGA NYKRGVPSML GDDKGVVGRG
     GYKYFGRAKD LPGVKELFTS QKADEAGRSE ERYERFRNLP PSYYGDEDED DGLLLEQERS
     AEEVEWNERF WNVVQGLKHD LQSRDDHDNM HLTDAEDDEH GGIQVPKIPR PSPRPLKQSV
     QAVQASLSAA YNQRFMEHAA TPAESTSKRA PQDELQTPAK RHKNEQGHPI PPTHHADAEP
     SRTTLEPPSV ADYSIFSPED LQPPPIPDRA AIEKLILQAK KKQLRQEYIG A