ID   IT1B_PSOTE              Reviewed;         172 AA.
AC   P32877; P01072;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Trypsin inhibitor 1B;
DE   AltName: Full=WTI-1B;
OS   Psophocarpus tetragonolobus (Winged bean) (Dolichos tetragonolobus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Psophocarpus.
OX   NCBI_TaxID=3891;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RX   PubMed=6643426; DOI=10.1093/oxfordjournals.jbchem.a134427;
RA   Yamamoto M., Hara S., Ikenaka T.;
RT   "Amino acid sequences of two trypsin inhibitors from winged bean seeds
RT   (Psophocarpus tetragonolobus (L)DC.).";
RL   J. Biochem. 94:849-863(1983).
CC   -!- FUNCTION: WTI-1B inhibits trypsin stoichiometrically.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   PIR; A01311; TIWDKB.
DR   AlphaFoldDB; P32877; -.
DR   SMR; P32877; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..172
FT                   /note="Trypsin inhibitor 1B"
FT                   /id="PRO_0000083303"
FT   SITE            64..65
FT                   /note="Reactive bond for trypsin"
FT   DISULFID        40..84
FT                   /evidence="ECO:0000250"
FT   DISULFID        133..139
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   172 AA;  19217 MW;  DB2F726F6FBBF108 CRC64;
     EPLLDSEGEL VRNGGTYYLL PDRWALGGGI EAAATGTETC PLTVVRSPNE VSVGEPLRIS
     SQLRSGFIPD YSLVRIGFAN PPKCAPSPWW TVVEDQPQQP SVKLSELKST KFDYLFKFEK
     VTSKFSSYKL KYCAKRDTCK DIGIYRDQKG YARLVVTDEN PLVVIFKKVE SS