ID   IT2_PSOTE               Reviewed;         182 AA.
AC   P25700;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 2.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Trypsin inhibitor 2;
DE   AltName: Full=WTI-2;
OS   Psophocarpus tetragonolobus (Winged bean) (Dolichos tetragonolobus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Psophocarpus.
OX   NCBI_TaxID=3891;
RN   [1]
RP   PROTEIN SEQUENCE, AND PYROGLUTAMATE FORMATION AT GLN-1.
RC   TISSUE=Seed;
RX   PubMed=1980416; DOI=10.1007/bf01024626;
RA   Caldwell J.B., Strike P.M., Kortt A.A.;
RT   "Amino acid sequence of the acidic Kunitz-type trypsin inhibitor from
RT   winged-bean seed [Psophocarpus tetragonolobus (L.) DC].";
RL   J. Protein Chem. 9:493-499(1990).
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; A37211; A37211.
DR   AlphaFoldDB; P25700; -.
DR   SMR; P25700; -.
DR   MEROPS; I03.011; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Pyrrolidone carboxylic acid; Serine protease inhibitor.
FT   CHAIN           1..182
FT                   /note="Trypsin inhibitor 2"
FT                   /id="PRO_0000083304"
FT   SITE            64..65
FT                   /note="Reactive bond for trypsin"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:1980416"
FT   DISULFID        40..84
FT                   /evidence="ECO:0000250"
FT   DISULFID        136..147
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   182 AA;  20394 MW;  2991A9C8B236D6AF CRC64;
     QELVDVEGKT VRNGGTYYLV PQLRPGGGGM EAAKVGNEDC PLTVVKSLDE NSNGEPIRIA
     SRLRSTFIPE YSLVNLGFAD PPKCAPSPFW TVVKDQSERL PSIKLGEYKD SELDYPFKFE
     RVYAASKMYA YKLLYCGSED EEEEMMCKDI GVYRDQEGYQ RLVVSKHNPL VVGFKKAESS
     TT