ID   ITFG2_BOVIN             Reviewed;         532 AA.
AC   Q27969;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=KICSTOR complex protein ITFG2 {ECO:0000305};
DE   AltName: Full=Adrenal medulla 50 kDa protein {ECO:0000312|EMBL:AAA17986.1};
DE   AltName: Full=Integrin-alpha FG-GAP repeat-containing protein 2 {ECO:0000250|UniProtKB:Q969R8};
GN   Name=ITFG2 {ECO:0000250|UniProtKB:Q969R8};
GN   Synonyms=ADP50 {ECO:0000312|EMBL:AAA17986.1};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Adrenal medulla;
RA   Nelson N., Nelson H., Mandiyan S.;
RT   "A cDNA encoding a protein of 50 kDa from bovine adrenal medulla.";
RL   Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: As part of the KICSTOR complex functions in the amino acid-
CC       sensing branch of the TORC1 signaling pathway. Recruits, in an amino
CC       acid-independent manner, the GATOR1 complex to the lysosomal membranes
CC       and allows its interaction with GATOR2 and the RAG GTPases. Functions
CC       upstream of the RAG GTPases and is required to negatively regulate
CC       mTORC1 signaling in absence of amino acids. In absence of the KICSTOR
CC       complex mTORC1 is constitutively localized to the lysosome and
CC       activated. The KICSTOR complex is also probably involved in the
CC       regulation of mTORC1 by glucose. {ECO:0000250|UniProtKB:Q969R8}.
CC   -!- SUBUNIT: Part of the KICSTOR complex composed of KPTN, ITFG2, KICS2 and
CC       SZT2. SZT2 probably serves as a link between the other three proteins
CC       in the KICSTOR complex and may mediate the direct interaction with the
CC       GATOR complex via GATOR1. The KICSTOR complex interacts directly with
CC       the GATOR1 complex and most probably indirectly with the GATOR2 complex
CC       in an amino acid-independent manner. {ECO:0000250|UniProtKB:Q969R8}.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q969R8}.
CC       Note=Localization to lysosomes is amino acid-independent.
CC       {ECO:0000250|UniProtKB:Q969R8}.
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DR   EMBL; U04706; AAA17986.1; -; mRNA.
DR   RefSeq; NP_776987.1; NM_174562.2.
DR   AlphaFoldDB; Q27969; -.
DR   STRING; 9913.ENSBTAP00000010004; -.
DR   GeneID; 282275; -.
DR   KEGG; bta:282275; -.
DR   CTD; 55846; -.
DR   eggNOG; ENOG502QUBC; Eukaryota.
DR   InParanoid; Q27969; -.
DR   OrthoDB; 1128880at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0140007; C:KICSTOR complex; ISS:UniProtKB.
DR   GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR   GO; GO:0034198; P:cellular response to amino acid starvation; ISS:UniProtKB.
DR   GO; GO:0042149; P:cellular response to glucose starvation; ISS:UniProtKB.
DR   GO; GO:1904262; P:negative regulation of TORC1 signaling; ISS:UniProtKB.
DR   GO; GO:0032006; P:regulation of TOR signaling; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR028994; Integrin_alpha_N.
DR   InterPro; IPR031793; KICSTOR_ITFG2.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR16317; PTHR16317; 1.
DR   Pfam; PF15907; Itfg2; 1.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   SUPFAM; SSF69318; SSF69318; 1.
PE   2: Evidence at transcript level;
KW   Lysosome; Membrane; Phosphoprotein; Reference proteome; Repeat.
FT   CHAIN           1..532
FT                   /note="KICSTOR complex protein ITFG2"
FT                   /id="PRO_0000064447"
FT   REPEAT          19..48
FT                   /note="FG-GAP 1; atypical"
FT   REPEAT          126..155
FT                   /note="FG-GAP 2; atypical"
FT   REGION          248..271
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         104
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q969R8"
SQ   SEQUENCE   532 AA;  58160 MW;  B0B1BEE87194369B CRC64;
     MRSVSYVQRV ALEFSGSLFP HAICLGDVDN DTLNELVVGD TSGKLSVYKN DDSRPWLTCS
     CQGMLTCVGV GDVCNKGKNL VVAVSAEGWF HLCDLTPAKS LDGSGHHETL GGEEQRPVFK
     QHIPANTKVM LISDIDGDGR CELVVGYTDR VVRAFRWEDL GEGAEHPMGQ LVLLKKWMLE
     GQVDSLSVTP GPLGVPELMV SQPGCAYAIL LCTWNKDPGA TPTSEGPMEG HSGAPGCPGR
     CVAPDLWPHP QQERLHSPHR QHQASHSPDS SASGLFALCT LDGTLKLMEE ADRLLWSVQV
     DHQLFALEKL DVTGNGHEEV VACAWDGQTY IIDHNRTVVR FQVDENIRAF CAGLYACKDS
     RNSPCLVYVT FNQKIYVYWE VQLERMESTN LLKVLEAQPS SGSCWESWAW ILMSCPPPAP
     CFTKPSTIQT SLHSAPPRAP RTPPSWTGFL AEQGAFCTSP PPPPPQLSGI WKTGSAHYWG
     RMAAPLGCGD CRPPGRLGEG RDKLPLCPFP CVPHLSRQQA LPLPRAPYSC CL