ITFG2_MOUSE
ID ITFG2_MOUSE Reviewed; 443 AA.
AC Q91WI7; Q3UYJ5; Q8R148;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=KICSTOR complex protein ITFG2 {ECO:0000305};
DE AltName: Full=Integrin-alpha FG-GAP repeat-containing protein 2 {ECO:0000312|MGI:MGI:1915450};
GN Name=Itfg2 {ECO:0000312|MGI:MGI:1915450};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N; TISSUE=Eye, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: As part of the KICSTOR complex functions in the amino acid-
CC sensing branch of the TORC1 signaling pathway. Recruits, in an amino
CC acid-independent manner, the GATOR1 complex to the lysosomal membranes
CC and allows its interaction with GATOR2 and the RAG GTPases. Functions
CC upstream of the RAG GTPases and is required to negatively regulate
CC mTORC1 signaling in absence of amino acids. In absence of the KICSTOR
CC complex mTORC1 is constitutively localized to the lysosome and
CC activated. The KICSTOR complex is also probably involved in the
CC regulation of mTORC1 by glucose. {ECO:0000250|UniProtKB:Q969R8}.
CC -!- SUBUNIT: Part of the KICSTOR complex composed of KPTN, ITFG2, KICS2 and
CC SZT2. SZT2 probably serves as a link between the other three proteins
CC in the KICSTOR complex and may mediate the direct interaction with the
CC GATOR complex via GATOR1. The KICSTOR complex interacts directly with
CC the GATOR1 complex and most probably indirectly with the GATOR2 complex
CC in an amino acid-independent manner. {ECO:0000250|UniProtKB:Q969R8}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q969R8}.
CC Note=Localization to lysosomes is amino acid-independent.
CC {ECO:0000250|UniProtKB:Q969R8}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q91WI7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91WI7-2; Sequence=VSP_026003;
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DR EMBL; AK134630; BAE22217.1; -; mRNA.
DR EMBL; AK147728; BAE28098.1; -; mRNA.
DR EMBL; BC014833; AAH14833.1; -; mRNA.
DR EMBL; BC025492; AAH25492.1; -; mRNA.
DR CCDS; CCDS20572.1; -. [Q91WI7-1]
DR RefSeq; NP_598688.1; NM_133927.1. [Q91WI7-1]
DR AlphaFoldDB; Q91WI7; -.
DR STRING; 10090.ENSMUSP00000001559; -.
DR iPTMnet; Q91WI7; -.
DR PhosphoSitePlus; Q91WI7; -.
DR SwissPalm; Q91WI7; -.
DR EPD; Q91WI7; -.
DR jPOST; Q91WI7; -.
DR MaxQB; Q91WI7; -.
DR PaxDb; Q91WI7; -.
DR PRIDE; Q91WI7; -.
DR ProteomicsDB; 301695; -. [Q91WI7-1]
DR ProteomicsDB; 301696; -. [Q91WI7-2]
DR Antibodypedia; 22171; 276 antibodies from 22 providers.
DR DNASU; 101142; -.
DR Ensembl; ENSMUST00000001559; ENSMUSP00000001559; ENSMUSG00000001518. [Q91WI7-1]
DR GeneID; 101142; -.
DR KEGG; mmu:101142; -.
DR UCSC; uc009edo.1; mouse. [Q91WI7-1]
DR UCSC; uc012etc.1; mouse. [Q91WI7-2]
DR CTD; 55846; -.
DR MGI; MGI:1915450; Itfg2.
DR VEuPathDB; HostDB:ENSMUSG00000001518; -.
DR eggNOG; ENOG502QUBC; Eukaryota.
DR GeneTree; ENSGT00390000005378; -.
DR HOGENOM; CLU_021730_0_0_1; -.
DR InParanoid; Q91WI7; -.
DR OMA; GGQNSPC; -.
DR OrthoDB; 1128880at2759; -.
DR PhylomeDB; Q91WI7; -.
DR TreeFam; TF329010; -.
DR Reactome; R-MMU-9639288; Amino acids regulate mTORC1.
DR BioGRID-ORCS; 101142; 6 hits in 72 CRISPR screens.
DR ChiTaRS; Itfg2; mouse.
DR PRO; PR:Q91WI7; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q91WI7; protein.
DR Bgee; ENSMUSG00000001518; Expressed in retinal neural layer and 173 other tissues.
DR ExpressionAtlas; Q91WI7; baseline and differential.
DR Genevisible; Q91WI7; MM.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0140007; C:KICSTOR complex; ISS:UniProtKB.
DR GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:MGI.
DR GO; GO:0034198; P:cellular response to amino acid starvation; ISS:UniProtKB.
DR GO; GO:0042149; P:cellular response to glucose starvation; ISS:UniProtKB.
DR GO; GO:0002314; P:germinal center B cell differentiation; IMP:MGI.
DR GO; GO:1904262; P:negative regulation of TORC1 signaling; ISS:UniProtKB.
DR GO; GO:0032006; P:regulation of TOR signaling; IBA:GO_Central.
DR InterPro; IPR028994; Integrin_alpha_N.
DR InterPro; IPR031793; KICSTOR_ITFG2.
DR PANTHER; PTHR16317; PTHR16317; 1.
DR Pfam; PF15907; Itfg2; 1.
DR SUPFAM; SSF69318; SSF69318; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Lysosome; Membrane; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..443
FT /note="KICSTOR complex protein ITFG2"
FT /id="PRO_0000289293"
FT REPEAT 19..48
FT /note="FG-GAP 1; atypical"
FT REPEAT 125..154
FT /note="FG-GAP 2; atypical"
FT MOD_RES 104
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q969R8"
FT MOD_RES 219
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q969R8"
FT VAR_SEQ 409..443
FT /note="VDPEDLPAVCTLLHQTLYHPDQPLQCTPSSFQDPT -> ILKISLQSVPCFI
FT RLSTIRTSHYSVPPQASRTPPSWARPL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026003"
FT CONFLICT 257
FT /note="L -> Q (in Ref. 1; BAE22217)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 443 AA; 48971 MW; 9F13DBCF34C2898C CRC64;
MRSVSYVQRV ALDFSGSLFP HAICLGDVDN DALNELVVGD TSGKLSVYKN DDSRPWLTCM
CQGMLTCVGV GDVCNKGKNL VVAVSAEGWL HLFDLTPTKA LDASGHHETL GEEQRPVFKQ
HIPANTKVML ISDIDGDGCY ELVVGYTDRV VRAFRWEELA EGPEHLAGQL VSLKKWMLEG
QVDSLSVTPG PLGVPELVVS QPGCAYAVLL CTWNKDTGSP PASEEATGDS RETPAARDVV
LHQTSGRIHN KNVSTHLIGN IRQGHNPEGG NAGLFALCTL DGTLKLMQEA DKLLWSVQVD
HQLFALEKLD VTGNGLEEVV ACAWDGQTYI IDHNRTVVRF QVDENIRAFC AGQYACKEGR
NSPCLVYVTF NQKIYVYWEV QLERMESTNL LKLLEAEPEY HRLLQELRVD PEDLPAVCTL
LHQTLYHPDQ PLQCTPSSFQ DPT
