ITIH4_BOVIN
ID ITIH4_BOVIN Reviewed; 916 AA.
AC Q3T052;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Inter-alpha-trypsin inhibitor heavy chain H4;
DE Short=ITI heavy chain H4;
DE Short=ITI-HC4;
DE Short=Inter-alpha-inhibitor heavy chain 4;
DE Flags: Precursor;
GN Name=ITIH4;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Testis;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF 389-413, INDUCTION, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=15213118; DOI=10.1128/iai.72.7.3777-3782.2004;
RA Pineiro M., Andres M., Iturralde M., Carmona S., Hirvonen J., Pyorala S.,
RA Heegaard P.M., Tjornehoj K., Lampreave F., Pineiro A., Alava M.A.;
RT "ITIH4 (inter-alpha-trypsin inhibitor heavy chain 4) is a new acute-phase
RT protein isolated from cattle during experimental infection.";
RL Infect. Immun. 72:3777-3782(2004).
RN [3]
RP GLYCOSYLATION AT SER-683; THR-705; THR-706 AND THR-708, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RX PubMed=19674964; DOI=10.1074/mcp.m900211-mcp200;
RA Darula Z., Medzihradszky K.F.;
RT "Affinity enrichment and characterization of mucin core-1 type
RT glycopeptides from bovine serum.";
RL Mol. Cell. Proteomics 8:2515-2526(2009).
CC -!- FUNCTION: Type II acute-phase protein (APP) involved in inflammatory
CC responses to trauma. May also play a role in liver development or
CC regeneration. {ECO:0000269|PubMed:15213118}.
CC -!- SUBUNIT: Interacts (via C-terminus) with DNAJC1 (via SANT 2 domain).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15213118}.
CC -!- INDUCTION: Levels of ITIH4 in serum increase 3- to 12-fold on
CC inoculation with various bacteria which induce mastitis. Peak levels
CC are reached around 42h-72 h after infection.
CC {ECO:0000269|PubMed:15213118}.
CC -!- PTM: Appears to be both N- and O-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ITIH family. {ECO:0000305}.
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DR EMBL; BC102561; AAI02562.1; -; mRNA.
DR RefSeq; NP_001015590.2; NM_001015590.3.
DR AlphaFoldDB; Q3T052; -.
DR SMR; Q3T052; -.
DR STRING; 9913.ENSBTAP00000010330; -.
DR GlyConnect; 801; 2 O-Linked glycans (4 sites).
DR iPTMnet; Q3T052; -.
DR PaxDb; Q3T052; -.
DR PeptideAtlas; Q3T052; -.
DR PRIDE; Q3T052; -.
DR GeneID; 513700; -.
DR KEGG; bta:513700; -.
DR CTD; 3700; -.
DR eggNOG; ENOG502QPS2; Eukaryota.
DR InParanoid; Q3T052; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0006953; P:acute-phase response; IEP:UniProtKB.
DR GO; GO:0030212; P:hyaluronan metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR010600; ITI_HC_C.
DR InterPro; IPR013694; VIT.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF06668; ITI_HC_C; 1.
DR Pfam; PF08487; VIT; 1.
DR Pfam; PF00092; VWA; 1.
DR SMART; SM00609; VIT; 1.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS51468; VIT; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 1: Evidence at protein level;
KW Acute phase; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..916
FT /note="Inter-alpha-trypsin inhibitor heavy chain H4"
FT /id="PRO_0000285684"
FT DOMAIN 28..149
FT /note="VIT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00801"
FT DOMAIN 275..458
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT REGION 597..616
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 678..701
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 82
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 208
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 518
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 683
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000269|PubMed:19674964"
FT CARBOHYD 705
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:19674964"
FT CARBOHYD 706
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:19674964"
FT CARBOHYD 708
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000269|PubMed:19674964"
FT DISULFID 733..911
FT /evidence="ECO:0000250"
FT CONFLICT 390
FT /note="T -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 397
FT /note="L -> S (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 411..412
FT /note="HS -> QV (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 916 AA; 101513 MW; FC1AEC0745DB7086 CRC64;
MKTPAPGRIH SIVLVLLSLA VLQTSKAQKV QNDIDIYSLT VDSKVSSRFA HTVITSRVVN
KADAVREATF QMELPKKAFI TNFSMVIDGV TYPGNIKEKA AAQEQYSAAV ARGESAGLVR
ATGRKTEQFQ VSVSVAPAAK VTFELVYEEL LARHLGAYEL LLKVRPQQLV KHLQMDIHIF
EPQGISFLET ESTFMTNKLA EALTTSQNKT KAHVRFKPTL SQQQKYPEKQ DTVIDGSFIV
RYDVDRPLSG GSIQIENGYF VHYFAPDSLS TIPKNVIFVI DKSGSMMGRK IKQTREALIK
ILDDLSPHDQ FDLISFSSEA TTWKPLLVPA STENVNEAKS YATGIQAQGG TNINDAMLMA
VQLLEKANQE ELLPEGSITL IILLTDGDPT VGETNPLNIQ KNVRKAINGQ HSLFCLGFGF
DVSYAFLEKM ALENGGLARR IYEDSDSALQ LQDFYQEVAN PLMTSVAFEY PSNAVESVTQ
DTFRVFFKGS ELVVAGKLRE QSPDVLLAQI RGQLHRENIT YMMMSHVAEQ EEMFRSPKYI
FHSFIERLWA YLTIQQLLEQ MVSALDAEKQ ALEARALSLS LSYSFVTPLT SMVITKPEGQ
EQSQVAEKPV EDESRGSRVY LGPMRFGHSV GDRTSRKPGG GLKLLNGTPL FGPPGPPAAA
SPFHRMTSRL VLPELMSPLA PASAPSPTSG PGGASHDTDF RIKGTTPTAL PFAPVQAPSV
ILPLPGQSVD RLCVDLRRPQ ELVNLLSDPD QGVEVTGHFE TAKARFSWIE VTFENPQVQI
HASPEHVVMT RNRRNSAYKW KETLYSVMPG LKVTMDKEGL LLLSRPDRVT IGLLFWDGPG
KGLRLLLQNT DRFSSHVSGT LGQFYQDVLW GPLDTADDSK RTLKVQGRDY SATRELKLDY
QESPPGKEIS CWSVEL
