ID   ITI_FAGTA               Reviewed;          86 AA.
AC   P86971;
DT   21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Trypsin inhibitor {ECO:0000303|PubMed:21544554};
DE            Short=FtTI {ECO:0000303|PubMed:21544554};
OS   Fagopyrum tataricum (Tartarian buckwheat) (Polygonum tataricum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Polygonaceae; Polygonoideae; Fagopyreae; Fagopyrum.
OX   NCBI_TaxID=62330;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MASS
RP   SPECTROMETRY, AND DISULFIDE BONDS.
RC   TISSUE=Seed {ECO:0000269|PubMed:21544554};
RX   PubMed=21544554; DOI=10.1007/s12010-011-9257-4;
RA   Ruan J.J., Zhou M.L., Chen H., Shao J.R.;
RT   "Identification and Characterization of a Trypsin Inhibitor from Fagopyrum
RT   tataricum Seeds.";
RL   Appl. Biochem. Biotechnol. 0:0-0(2011).
CC   -!- FUNCTION: Serine protease inhibitor which is active against trypsin.
CC       Displays strong antifungal activity against a number of phytopathogenic
CC       fungi including M.melonis, A.cucumerina, A.solani, C.glaeosporioides
CC       and P.capsici. {ECO:0000269|PubMed:21544554}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.0 at 37 degrees Celsius. Maintains over 90% of its
CC         inhibitory activity from pH 3 to 10. {ECO:0000269|PubMed:21544554};
CC       Temperature dependence:
CC         Active between 10 and 60 degrees Celsius. 84% activity retained when
CC         heated for 30 minutes at 80 degrees Celsius. Incubation at
CC         temperatures above 80 degrees Celsius rapidly decreases inhibitory
CC         activity. {ECO:0000269|PubMed:21544554};
CC   -!- MASS SPECTROMETRY: Mass=13838.50; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:21544554};
CC   -!- SIMILARITY: Belongs to the protease inhibitor I13 (potato type I serine
CC       protease inhibitor) family. {ECO:0000255}.
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DR   AlphaFoldDB; P86971; -.
DR   SMR; P86971; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEA:InterPro.
DR   InterPro; IPR000864; Prot_inh_pot1.
DR   InterPro; IPR036354; Prot_inh_pot1_sf.
DR   PANTHER; PTHR33091; PTHR33091; 1.
DR   Pfam; PF00280; potato_inhibit; 1.
DR   SUPFAM; SSF54654; SSF54654; 1.
DR   PROSITE; PS00285; POTATO_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Plant defense; Protease inhibitor; Serine protease inhibitor.
FT   PEPTIDE         1..86
FT                   /note="Trypsin inhibitor"
FT                   /evidence="ECO:0000269|PubMed:21544554"
FT                   /id="PRO_0000412717"
FT   SITE            61..62
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250|UniProtKB:P01051"
FT   DISULFID        8..65
FT                   /evidence="ECO:0000269|PubMed:21544554"
FT   DISULFID        49..58
FT                   /evidence="ECO:0000269|PubMed:21544554"
SQ   SEQUENCE   86 AA;  9293 MW;  BF417A5EAD2EE2F0 CRC64;
     LIYAKVECLT TGVRTYVGKQ SWPELVGTKG KTAAATIDKE NTHVTAVLCP PLTTLAACRT
     FDFRCDRVRV LINRIGGVVT KTPTVG