ITN1_ARATH
ID ITN1_ARATH Reviewed; 590 AA.
AC Q9C7A2; Q8LED2; Q9LHH2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Ankyrin repeat-containing protein ITN1;
DE AltName: Full=Protein INCREASED TOLERANCE TO NACL {ECO:0000303|PubMed:18643991};
GN Name=ITN1 {ECO:0000303|PubMed:18643991}; OrderedLocusNames=At3g12360;
GN ORFNames=MQC3.17, T2E22.31;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=18643991; DOI=10.1111/j.1365-313x.2008.03614.x;
RA Sakamoto H., Matsuda O., Iba K.;
RT "ITN1, a novel gene encoding an ankyrin-repeat protein that affects the
RT ABA-mediated production of reactive oxygen species and is involved in salt-
RT stress tolerance in Arabidopsis thaliana.";
RL Plant J. 56:411-422(2008).
RN [6]
RP INTERACTION WITH REM19/RTV1, AND SUBCELLULAR LOCATION.
RX PubMed=22664102; DOI=10.1016/j.bbrc.2012.05.136;
RA Sakamoto H., Sakata K., Kusumi K., Kojima M., Sakakibara H., Iba K.;
RT "Interaction between a plasma membrane-localized ankyrin-repeat protein
RT ITN1 and a nuclear protein RTV1.";
RL Biochem. Biophys. Res. Commun. 423:392-397(2012).
CC -!- FUNCTION: Involved in salt stress tolerance. May act through abscisic
CC acid (ABA) signaling pathways and promote reactive oxygen species (ROS)
CC production. {ECO:0000269|PubMed:18643991}.
CC -!- SUBUNIT: Interacts with REM19/RTV1. {ECO:0000269|PubMed:22664102}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18643991,
CC ECO:0000269|PubMed:22664102}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoots, leaf vasculature and
CC stems. {ECO:0000269|PubMed:18643991}.
CC -!- INDUCTION: By salt and drought stresses. {ECO:0000269|PubMed:18643991}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant seedlings show increased salt-stress tolerance.
CC {ECO:0000269|PubMed:18643991}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM62711.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB03143.1; Type=Erroneous gene model prediction; Note=The predicted gene At3g12350 has been split into 2 genes: At3g12350 and At3g12360.; Evidence={ECO:0000305};
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DR EMBL; AP002047; BAB03143.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC069474; AAG51002.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75188.1; -; Genomic_DNA.
DR EMBL; AY085485; AAM62711.1; ALT_INIT; mRNA.
DR RefSeq; NP_187842.1; NM_112071.3.
DR AlphaFoldDB; Q9C7A2; -.
DR SMR; Q9C7A2; -.
DR BioGRID; 5748; 2.
DR STRING; 3702.AT3G12360.1; -.
DR PaxDb; Q9C7A2; -.
DR PRIDE; Q9C7A2; -.
DR ProteomicsDB; 238956; -.
DR EnsemblPlants; AT3G12360.1; AT3G12360.1; AT3G12360.
DR GeneID; 820414; -.
DR Gramene; AT3G12360.1; AT3G12360.1; AT3G12360.
DR KEGG; ath:AT3G12360; -.
DR Araport; AT3G12360; -.
DR TAIR; locus:2092522; AT3G12360.
DR eggNOG; KOG0504; Eukaryota.
DR HOGENOM; CLU_000134_49_0_1; -.
DR InParanoid; Q9C7A2; -.
DR OrthoDB; 570510at2759; -.
DR PhylomeDB; Q9C7A2; -.
DR PRO; PR:Q9C7A2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9C7A2; baseline and differential.
DR Genevisible; Q9C7A2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IPI:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR Gene3D; 1.25.40.20; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR026961; PGG_dom.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF13962; PGG; 1.
DR SMART; SM00248; ANK; 7.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 5.
PE 1: Evidence at protein level;
KW ANK repeat; Cell membrane; Membrane; Reference proteome; Repeat;
KW Stress response; Transmembrane; Transmembrane helix.
FT CHAIN 1..590
FT /note="Ankyrin repeat-containing protein ITN1"
FT /id="PRO_0000274930"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 500..520
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 531..551
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 73..102
FT /note="ANK 1"
FT REPEAT 128..157
FT /note="ANK 2"
FT REPEAT 163..192
FT /note="ANK 3"
FT REPEAT 197..226
FT /note="ANK 4"
FT REPEAT 231..260
FT /note="ANK 5"
FT REPEAT 265..294
FT /note="ANK 6"
FT REPEAT 299..329
FT /note="ANK 7"
FT REGION 25..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 183
FT /note="L -> S (in Ref. 4; AAM62711)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 590 AA; 64135 MW; 979C333576EDD99E CRC64;
MAASSYVDGE RDMEKGGMIL LQSSENQNPM IDPSPTPSPS ATATAPALVL SNSGKRMDQA
GKKKYVKQVT GRHNDTELHL AAQRGDLAAV QQILKDINSQ MEGILSGEEF DAEVAEIRAS
IVNEVNELGE TALFTAADKG HLDVVKELLK YSSRESIAKK NRSGYDPLHI AAIQGHHAIV
EVLLDHDATL SQTFGPSNAT PLVSAAMRGH TEVVNQLLSK AGNLLEISRS NNKNALHLAA
RQGHVEVIKA LLSKDPQLAR RIDKKGQTAL HMAVKGQSSE VVKLLLDADP AIVMQPDKSC
NTALHVATRK KRAEIVELLL SLPDTNANTL TRDHKTALDI AEGLPLSEES SYIKECLARS
GALRANELNQ PRDELRSTVT QIKNDVHIQL EQTKRTNKNV HNISKELRKL HREGINNATN
SVTVVAVLFA TVAFAAIFTV PGGDNNDGSA VVVGRASFKI FFIFNALALF TSLAVVVVQI
TLVRGETKAE KRVVEVINKL MWLASMCTSV AFLASSYIVV GRKNEWAAEL VTVVGGVIMA
GVLGTMTYYV VKSKRTRSMR KKVKSARRSG SNSWHHSDFS NSEVDPIFAI
