ID   ITR1_JATCU              Reviewed;          27 AA.
AC   C0HJF7;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   03-SEP-2014, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Trypsin inhibitor 1 {ECO:0000303|PubMed:24523715};
DE            Short=JcTI-I {ECO:0000303|PubMed:24523715};
DE   Flags: Fragment;
OS   Jatropha curcas (Barbados nut).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Jatropheae;
OC   Jatropha.
OX   NCBI_TaxID=180498;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT,
RP   DISULFIDE BONDS, GLYCOSYLATION, AND MASS SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000269|PubMed:24523715};
RX   PubMed=24523715; DOI=10.3389/fmicb.2014.00005;
RA   Costa H.P., Oliveira J.T., Sousa D.O., Morais J.K., Moreno F.B.,
RA   Monteiro-Moreira A.C., Viegas R.A., Vasconcelos I.M.;
RT   "JcTI-I: a novel trypsin inhibitor from Jatropha curcas seed cake with
RT   potential for bacterial infection treatment.";
RL   Front. Microbiol. 5:5-5(2014).
CC   -!- FUNCTION: Inhibits trypsin (IC(50)=1.25 uM) but not chymotrypsin or
CC       papain. Has antibacterial activity against S.enterica ATCC 10708 (MIC=5
CC       ug/ml) and S.aureus ATCC 25923 (MIC=5 ug/ml) but not against B.subtilis
CC       ATCC 6633 or P.aeruginosa ATCC 25619. Has no hemolytic activity against
CC       human erythrocytes. Is not toxic to mice.
CC       {ECO:0000269|PubMed:24523715}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Trypsin inhibitory activity is stable after incubation at pH 2 to pH
CC         10 at 60 degrees Celsius for 1 hour. {ECO:0000269|PubMed:24523715};
CC       Temperature dependence:
CC         Trypsin inhibitory activity is stable after incubation at 90 degrees
CC         Celsius for up to 2 hours. {ECO:0000269|PubMed:24523715};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:24523715, ECO:0000305}.
CC   -!- PTM: Contains disulfide bonds. {ECO:0000269|PubMed:24523715}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:24523715}.
CC   -!- MASS SPECTROMETRY: Mass=10252; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:24523715};
CC   -!- MISCELLANEOUS: The monomer may consist of two smaller chains which are
CC       linked by at least one disulfide bond. {ECO:0000303|PubMed:24523715}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 6.6.
CC       {ECO:0000269|PubMed:24523715}.
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DR   AlphaFoldDB; C0HJF7; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0010951; P:negative regulation of endopeptidase activity; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Glycoprotein; Protease inhibitor; Serine protease inhibitor.
FT   CHAIN           1..>27
FT                   /note="Trypsin inhibitor 1"
FT                   /id="PRO_0000430166"
FT   NON_TER         27
FT                   /evidence="ECO:0000303|PubMed:24523715"
SQ   SEQUENCE   27 AA;  3262 MW;  35ECA80DD9E08683 CRC64;
     VRDICKKEAE RQDLSSCENY ITQRRGY