ID   4OT_COMTE               Reviewed;          63 AA.
AC   Q9RHM8;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=2-hydroxymuconate tautomerase;
DE            EC=5.3.2.6;
DE   AltName: Full=4-oxalocrotonate tautomerase;
DE            Short=4-OT;
GN   Name=aphI;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TA441;
RX   PubMed=10878134; DOI=10.1099/00221287-146-7-1707;
RA   Arai H., Ohishi T., Chang M.Y., Kudo T.;
RT   "Arrangement and regulation of the genes for meta-pathway enzymes required
RT   for degradation of phenol in Comamonas testosteroni TA441.";
RL   Microbiology 146:1707-1715(2000).
CC   -!- FUNCTION: Catalyzes the ketonization of 2-hydroxymuconate
CC       stereoselectively to yield 2-oxo-3-hexenedioate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-
CC         enedioate; Xref=Rhea:RHEA:33431, ChEBI:CHEBI:28080,
CC         ChEBI:CHEBI:64908; EC=5.3.2.6;
CC   -!- PATHWAY: Aromatic compound metabolism; salicylate degradation.
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the 4-oxalocrotonate tautomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AB029044; BAA88507.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RHM8; -.
DR   SMR; Q9RHM8; -.
DR   UniPathway; UPA00824; -.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046244; P:salicylic acid catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00491; 4Oxalocrotonate_Tautomerase; 1.
DR   Gene3D; 3.30.429.10; -; 1.
DR   InterPro; IPR004370; 4-oxalocrotonate_tautomerase.
DR   InterPro; IPR014347; Tautomerase/MIF_sf.
DR   InterPro; IPR018191; Tautomerase_Pseudo-typ.
DR   Pfam; PF01361; Tautomerase; 1.
DR   SUPFAM; SSF55331; SSF55331; 1.
DR   TIGRFAMs; TIGR00013; taut; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Isomerase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..63
FT                   /note="2-hydroxymuconate tautomerase"
FT                   /id="PRO_0000209511"
FT   ACT_SITE        2
FT                   /note="Proton acceptor; via imino nitrogen"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   63 AA;  6962 MW;  92AD4B0DAFA7799A CRC64;
     MPFAQIYMLE GRSEEQKKAV IEKVTRALVE AVGAPSANVR VWIHDVPKEN WGIAGVSAKE
     LGR