ID   ITR1_NICSY              Reviewed;          94 AA.
AC   Q02214;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Trypsin inhibitor 1;
DE   AltName: Full=NSTI-I;
DE   AltName: Full=Trypsin inhibitor I;
DE   Flags: Precursor;
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Xanthi NC; TISSUE=Protoplast;
RX   PubMed=1419848; DOI=10.1016/0925-4773(92)90004-4;
RA   Criqui M.-C., Plesse B., Durr A., Marbach J., Parmentier Y., Jamet E.,
RA   Fleck J.;
RT   "Characterization of genes expressed in mesophyll protoplasts of Nicotiana
RT   sylvestris before the re-initiation of the DNA replicational activity.";
RL   Mech. Dev. 38:121-132(1992).
CC   -!- FUNCTION: Stress response. Inhibits trypsin. May play a role in the
CC       reentering of protoplasts into the cell cycle.
CC   -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000305}. Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Mesophyll protoplasts and to a much lesser extent,
CC       roots and germinating seeds.
CC   -!- DEVELOPMENTAL STAGE: Before reinitiation of the DNA replicational
CC       activity.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I13 (potato type I serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   EMBL; M74102; AAA34067.1; -; mRNA.
DR   PIR; A56555; A56555.
DR   RefSeq; XP_009774947.1; XM_009776645.1.
DR   AlphaFoldDB; Q02214; -.
DR   SMR; Q02214; -.
DR   GeneID; 104224920; -.
DR   KEGG; nsy:104224920; -.
DR   eggNOG; ENOG502SAT0; Eukaryota.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEA:InterPro.
DR   InterPro; IPR000864; Prot_inh_pot1.
DR   InterPro; IPR036354; Prot_inh_pot1_sf.
DR   PANTHER; PTHR33091; PTHR33091; 1.
DR   Pfam; PF00280; potato_inhibit; 1.
DR   PRINTS; PR00292; POTATOINHBTR.
DR   SUPFAM; SSF54654; SSF54654; 1.
DR   PROSITE; PS00285; POTATO_INHIBITOR; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW   Serine protease inhibitor; Signal; Vacuole.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..94
FT                   /note="Trypsin inhibitor 1"
FT                   /id="PRO_0000025308"
FT   SITE            70..71
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        26..74
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   94 AA;  10464 MW;  31D1504CC8AF272D CRC64;
     MVKLALVVIF LLLASLFQPL TAQSSCPGNK KETWPELIGV PAKFAREIIQ KENSKLTNVP
     SVLNGSPVTK DFRCERVRLF VNVLDFVVQI PRVG