ITR1_SCHPO
ID ITR1_SCHPO Reviewed; 575 AA.
AC Q10286; Q9UU75;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Myo-inositol transporter 1;
GN Name=itr1; ORFNames=SPAC4F8.15, SPAC7D4.01;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=9560432; DOI=10.1007/s002940050334;
RA Niederberger C., Graub R., Schweingruber A.-M., Fankhauser H., Rusu M.,
RA Poitelea M., Edenharter L., Schweingruber M.E.;
RT "Exogenous inositol and genes responsible for inositol transport are
RT required for mating and sporulation in Shizosaccharomyces pombe.";
RL Curr. Genet. 33:255-261(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 136-280, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Transporter for myo-inositol. {ECO:0000269|PubMed:9560432}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + myo-inositol(out) = H(+)(in) + myo-inositol(in);
CC Xref=Rhea:RHEA:60364, ChEBI:CHEBI:15378, ChEBI:CHEBI:17268;
CC Evidence={ECO:0000305|PubMed:9560432};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein
CC {ECO:0000255}. Vacuole membrane {ECO:0000269|PubMed:16823372}; Multi-
CC pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; X98622; CAA67211.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB16718.1; -; Genomic_DNA.
DR EMBL; AB027770; BAA87074.1; -; Genomic_DNA.
DR PIR; T43400; T43400.
DR RefSeq; NP_593858.2; NM_001019287.2.
DR AlphaFoldDB; Q10286; -.
DR SMR; Q10286; -.
DR BioGRID; 279577; 4.
DR STRING; 4896.SPAC4F8.15.1; -.
DR iPTMnet; Q10286; -.
DR MaxQB; Q10286; -.
DR PaxDb; Q10286; -.
DR PRIDE; Q10286; -.
DR EnsemblFungi; SPAC4F8.15.1; SPAC4F8.15.1:pep; SPAC4F8.15.
DR GeneID; 2543145; -.
DR KEGG; spo:SPAC4F8.15; -.
DR PomBase; SPAC4F8.15; itr1.
DR VEuPathDB; FungiDB:SPAC4F8.15; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_001265_30_5_1; -.
DR InParanoid; Q10286; -.
DR OMA; VWILYMS; -.
DR PhylomeDB; Q10286; -.
DR Reactome; R-SPO-429593; Inositol transporters.
DR PRO; PR:Q10286; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005366; F:myo-inositol:proton symporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1904679; P:myo-inositol import across plasma membrane; IGI:PomBase.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 2.
PE 3: Inferred from homology;
KW Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..575
FT /note="Myo-inositol transporter 1"
FT /id="PRO_0000050453"
FT TOPO_DOM 1..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..129
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..156
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..186
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..215
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..246
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..349
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..376
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..441
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..486
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 487..507
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 508..510
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 532..575
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 432
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 575 AA; 62758 MW; 3B7C5EFF86C596AE CRC64;
MSISSKDFQN VTSAGFADDT FAADTFAADK KSPFESSVFE NKTQVLPVDS VSRLSNGARS
RSNSNISLSE PHALNDTVED QPVSKWVWVL AFAAGIGGLL FGYDTGVISG ALVVIGTSLG
GHELTNGGKE FITSATSLGA LLGGIIAGAL ADFFGRKPVI AIASIIIIVG SIVQVTAHHL
WHMIVGRFVI GWGVGIASLI IPLYLSEIAP SKIRGRLVII YVLLITAGQV IAYGIDTAFE
HVHNGWRWMV GLAMVPAAFQ LFILIWLPES PRLLVKKERS QEAYNTLARI YPTAHPYEIK
TKLYLIQEGV RDPFSGSRWQ KIVKTFKELY FNPSNFRALI LACGLQAMQQ LSGFNSLMYF
SSTIFEVVGF NNPTATGLII AATNFVFTIV AFGVIDFFGR RILLLLTVWG MIAALIVCAV
AFHFLPKDEN GNYTSGQSNA WAIVVLISMI VYVASYASGL GNLPWQQSEL FPMSVRGLGT
GMSTAVNWAG NLGIGASFLT LMSEITPTGT FALYGGLCFL GWLGALFCYP DLTDYTIEEI
GELLKHGFGV RESMRHLKRV RQERAWSRED KEQNN
