ID   ITR1_TRIKI              Reviewed;          65 AA.
AC   Q43667;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Trypsin inhibitor 1;
DE   AltName: Full=TTII;
DE   AltName: Full=Trypsin inhibitor I;
DE   Flags: Precursor;
OS   Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Trichosanthes.
OX   NCBI_TaxID=3677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Maximowicz; TISSUE=Root tuber;
RA   Ling M.H., Chi C.W., Shaw P.C.;
RT   "cDNA sequence of the Trichosanthes trypsin inhibitor.";
RL   Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Inhibits trypsin. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I7 (squash-type serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   EMBL; X82230; CAA57704.1; -; mRNA.
DR   PIR; S49486; S49486.
DR   AlphaFoldDB; Q43667; -.
DR   MEROPS; I07.020; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00150; PlantTI; 1.
DR   InterPro; IPR000737; Prot_inh_squash.
DR   InterPro; IPR011052; Proteinase_amylase_inhib_sf.
DR   Pfam; PF00299; Squash; 1.
DR   SUPFAM; SSF57027; SSF57027; 1.
DR   PROSITE; PS00286; SQUASH_INHIBITOR; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Knottin; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..?
FT                   /evidence="ECO:0000255"
FT   PROPEP          ?..34
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000033218"
FT   PEPTIDE         35..65
FT                   /note="Trypsin inhibitor 1"
FT                   /id="PRO_0000033219"
FT   SITE            41..42
FT                   /note="Reactive bond"
FT   DISULFID        39..56
FT                   /evidence="ECO:0000250"
FT   DISULFID        46..58
FT                   /evidence="ECO:0000250"
FT   DISULFID        52..64
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   65 AA;  6877 MW;  04473A36A4AC0605 CRC64;
     MAAFVESARA GAGADEVIQL VSDGVNEYSE KMMEGVVACP RILMPCKVND DCLRGCKCLS
     NGYCG