ID   ITR2_LUFAE              Reviewed;          30 AA.
AC   P25850;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Trypsin inhibitor 2;
DE   AltName: Full=LCTI-II;
DE   AltName: Full=Trypsin inhibitor II;
OS   Luffa aegyptiaca (Sponge gourd) (Luffa cylindrica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Luffa.
OX   NCBI_TaxID=3670;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RX   PubMed=1368760; DOI=10.1271/bbb1961.55.2641;
RA   Hatakeyama T., Hiraoka M., Funatsu G.;
RT   "Amino acid sequences of the two smallest trypsin inhibitors from sponge
RT   gourd seeds.";
RL   Agric. Biol. Chem. 55:2641-2642(1991).
CC   -!- FUNCTION: Inhibits trypsin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I7 (squash-type serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   PIR; JS0579; JS0579.
DR   AlphaFoldDB; P25850; -.
DR   SMR; P25850; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00150; PlantTI; 1.
DR   InterPro; IPR000737; Prot_inh_squash.
DR   InterPro; IPR011052; Proteinase_amylase_inhib_sf.
DR   Pfam; PF00299; Squash; 1.
DR   PRINTS; PR00293; SQUASHINHBTR.
DR   SUPFAM; SSF57027; SSF57027; 1.
DR   PROSITE; PS00286; SQUASH_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Protease inhibitor;
KW   Secreted; Serine protease inhibitor.
FT   PEPTIDE         1..30
FT                   /note="Trypsin inhibitor 2"
FT                   /id="PRO_0000044383"
FT   SITE            5..6
FT                   /note="Reactive bond"
FT   DISULFID        3..20
FT                   /evidence="ECO:0000250"
FT   DISULFID        10..22
FT                   /evidence="ECO:0000250"
FT   DISULFID        16..29
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   30 AA;  3311 MW;  7A53278DF00C8DC0 CRC64;
     RICPRILMEC SSDSDCLAEC ICLEQDGFCG