ID   ITR3_LUFAE              Reviewed;          29 AA.
AC   Q9S8I2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Trypsin inhibitor 3;
DE   AltName: Full=LCTI-III;
DE   AltName: Full=Trypsin inhibitor III;
OS   Luffa aegyptiaca (Sponge gourd) (Luffa cylindrica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Luffa.
OX   NCBI_TaxID=3670;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=7896727; DOI=10.1093/oxfordjournals.jbchem.a124621;
RA   Hayashi K., Takehisa T., Hamato N., Takano R., Hara S., Miyata T., Kato H.;
RT   "Inhibition of serine proteases of the blood coagulation system by squash
RT   family protease inhibitors.";
RL   J. Biochem. 116:1013-1018(1994).
CC   -!- FUNCTION: Inhibits trypsin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I7 (squash-type serine
CC       protease inhibitor) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; Q9S8I2; -.
DR   SMR; Q9S8I2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00150; PlantTI; 1.
DR   InterPro; IPR000737; Prot_inh_squash.
DR   InterPro; IPR011052; Proteinase_amylase_inhib_sf.
DR   Pfam; PF00299; Squash; 1.
DR   PRINTS; PR00293; SQUASHINHBTR.
DR   SUPFAM; SSF57027; SSF57027; 1.
DR   PROSITE; PS00286; SQUASH_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Protease inhibitor;
KW   Secreted; Serine protease inhibitor.
FT   PEPTIDE         1..29
FT                   /note="Trypsin inhibitor 3"
FT                   /id="PRO_0000044384"
FT   SITE            5..6
FT                   /note="Reactive bond"
FT   DISULFID        3..20
FT                   /evidence="ECO:0000250"
FT   DISULFID        10..22
FT                   /evidence="ECO:0000250"
FT   DISULFID        16..28
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   29 AA;  3182 MW;  D5E0FC42CE1DC01F CRC64;
     RICPRILMEC SSDSDCLAEC ICLENGFCG