ID   ITR3_MOMCH              Reviewed;          30 AA.
AC   Q9S747;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Trypsin inhibitor 3;
DE   AltName: Full=MCTI-III;
DE   AltName: Full=Trypsin inhibitor III;
OS   Momordica charantia (Bitter gourd) (Balsam pear).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Momordiceae; Momordica.
OX   NCBI_TaxID=3673;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=7896727; DOI=10.1093/oxfordjournals.jbchem.a124621;
RA   Hayashi K., Takehisa T., Hamato N., Takano R., Hara S., Miyata T., Kato H.;
RT   "Inhibition of serine proteases of the blood coagulation system by squash
RT   family protease inhibitors.";
RL   J. Biochem. 116:1013-1018(1994).
RN   [2]
RP   PROTEIN SEQUENCE.
RX   PubMed=7608135; DOI=10.1093/jb/117.2.432;
RA   Hamato N., Koshiba T., Pham T.N., Tatsumi Y., Nakamura D., Takano R.,
RA   Hayashi K., Hong Y.M., Hara S.;
RT   "Trypsin and elastase inhibitors from bitter gourd (Momordica charantia
RT   LINN.) seeds: purification, amino acid sequences, and inhibitory activities
RT   of four new inhibitors.";
RL   J. Biochem. 117:432-437(1995).
CC   -!- FUNCTION: Inhibits trypsin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I7 (squash-type serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   AlphaFoldDB; Q9S747; -.
DR   SMR; Q9S747; -.
DR   Proteomes; UP000504603; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00150; PlantTI; 1.
DR   InterPro; IPR000737; Prot_inh_squash.
DR   InterPro; IPR011052; Proteinase_amylase_inhib_sf.
DR   Pfam; PF00299; Squash; 1.
DR   SUPFAM; SSF57027; SSF57027; 1.
DR   PROSITE; PS00286; SQUASH_INHIBITOR; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Protease inhibitor;
KW   Reference proteome; Secreted; Serine protease inhibitor.
FT   PEPTIDE         1..30
FT                   /note="Trypsin inhibitor 3"
FT                   /id="PRO_0000044388"
FT   SITE            6..7
FT                   /note="Reactive bond"
FT   DISULFID        4..21
FT                   /evidence="ECO:0000250"
FT   DISULFID        11..23
FT                   /evidence="ECO:0000250"
FT   DISULFID        17..29
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   30 AA;  3286 MW;  D5E359C734A64E9A CRC64;
     ERGCPRILKQ CKQDSDCPGE CICMAHGFCG