ID   ITRY_BAUVA              Reviewed;         174 AA.
AC   P83595;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Trypsin inhibitor BvTI;
OS   Bauhinia variegata (Purple orchid tree) (Phanera variegata).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Cercidoideae; Cercideae; Bauhiniinae;
OC   Bauhinia.
OX   NCBI_TaxID=167791;
RN   [1]
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Cotyledon;
RX   PubMed=12678803; DOI=10.2174/0929867033457548;
RA   Oliva M.L.V., Andrade S.A., Juliano M.A., Sallai R.C., Torquato R.J.,
RA   Sampaio M.U., Pott V.J., Sampaio C.A.M.;
RT   "Kinetic characterization of factor Xa binding using a quenched fluorescent
RT   substrate based on the reactive site of factor Xa inhibitor from Bauhinia
RT   ungulata seeds.";
RL   Curr. Med. Chem. 10:1085-1093(2003).
CC   -!- FUNCTION: Inhibits bovine trypsin and chymotrypsin, and human plasmin,
CC       plasma kallikrein and factor XIIa. {ECO:0000269|PubMed:12678803}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   AlphaFoldDB; P83595; -.
DR   SMR; P83595; -.
DR   MEROPS; I03.027; -.
DR   PRIDE; P83595; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..174
FT                   /note="Trypsin inhibitor BvTI"
FT                   /id="PRO_0000083297"
FT   SITE            65..66
FT                   /note="Reactive bond for factor Xa"
FT   DISULFID        41..84
FT                   /evidence="ECO:0000250"
FT   DISULFID        131..138
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   174 AA;  19300 MW;  2169675A88A3AB4B CRC64;
     DTLLDTDGEV VRNNGGPYYI IPAFRGNGGG LTLTRVGSET CPRTVVQASS EHSDGLPVVI
     SALPRSLFIS TSWRVTIQFV EATCIPKPSF WHIPQDSELE GAVKVGASDE RFPLEFRIER
     VSEDTYKLMH CSSTSDSCRD LGISIDEEGN RRLVVRDENP LLVRFKKANQ DSEK