ID   ITRY_TRIKI              Reviewed;          41 AA.
AC   P01069;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Trypsin inhibitor;
OS   Trichosanthes kirilowii (Chinese snake gourd) (Chinese cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Trichosanthes.
OX   NCBI_TaxID=3677;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=6440850; DOI=10.1515/bchm2.1984.365.2.1211;
RA   Tan F., Zhang G., Mu J., Lin N., Chi C.;
RT   "Purification, characterization and sequence determination of a double-
RT   headed trypsin inhibitor peptide from Trichosanthes kirilowii (a Chinese
RT   medical herb).";
RL   Hoppe-Seyler's Z. Physiol. Chem. 365:1211-1217(1984).
CC   -!- FUNCTION: Has two active sites that simultaneously bind and inhibit
CC       trypsin.
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DR   PIR; A01308; TITZ.
DR   AlphaFoldDB; P01069; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..41
FT                   /note="Trypsin inhibitor"
FT                   /id="PRO_0000084273"
FT   SITE            20
FT                   /note="Interaction with trypsin"
FT   SITE            30
FT                   /note="Interaction with trypsin"
FT   DISULFID        15..26
FT   DISULFID        17..24
FT   DISULFID        29..37
FT                   /evidence="ECO:0000269|PubMed:6440850"
SQ   SEQUENCE   41 AA;  4531 MW;  371C84AAA8626A42 CRC64;
     LLMPVKPNDD RVIGCWCISR GYLCGCMPCK LNDDSLCGRK G