ID   IUCC_ECOLX              Reviewed;         580 AA.
AC   Q47318;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Aerobactin synthase;
DE            EC=6.3.2.39;
GN   Name=iucC;
OS   Escherichia coli.
OG   Plasmid IncFI ColV3-K30.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8003107; DOI=10.1006/jmbi.1994.1290;
RA   Martinez J.L., Herrero M., de Lorenzo V.;
RT   "The organization of intercistronic regions of the aerobactin operon of
RT   pColV-K30 may account for the differential expression of the iucABCD iutA
RT   genes.";
RL   J. Mol. Biol. 238:288-293(1994).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=2935523; DOI=10.1128/jb.165.2.570-578.1986;
RA   de Lorenzo V., Bindereif A., Paw B.H., Neilands J.B.;
RT   "Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in
RT   Escherichia coli K-12.";
RL   J. Bacteriol. 165:570-578(1986).
RN   [3]
RP   FUNCTION IN AEROBACTIN BIOSYNTHESIS, AND DISRUPTION PHENOTYPE.
RX   PubMed=3087960; DOI=10.1128/jb.167.1.350-355.1986;
RA   de Lorenzo V., Neilands J.B.;
RT   "Characterization of iucA and iucC genes of the aerobactin system of
RT   plasmid ColV-K30 in Escherichia coli.";
RL   J. Bacteriol. 167:350-355(1986).
CC   -!- FUNCTION: Catalyzes the attachment of the second N-acetyl-N-
CC       hydroxylysine to the carboxylic group of N-citryl-N-acetyl-N-
CC       hydroxylysine to yield aerobactin. Involved in the biosynthesis of the
CC       siderophore aerobactin which is a chelator that mediates the high-
CC       affinity iron transport systems induced under iron-stressed conditions.
CC       {ECO:0000269|PubMed:3087960}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + N(2)-citryl-N(6)-acetyl-N(6)-hydroxy-L-lysine + N(6)-
CC         acetyl-N(6)-hydroxy-L-lysine = aerobactin + AMP + diphosphate + H(+);
CC         Xref=Rhea:RHEA:32167, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58122, ChEBI:CHEBI:58396,
CC         ChEBI:CHEBI:63796, ChEBI:CHEBI:456215; EC=6.3.2.39;
CC   -!- PATHWAY: Siderophore biosynthesis; aerobactin biosynthesis.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are not able to produce
CC       aerobactin and accumulate N-citryl-N-acetyl-N-hydroxylysine.
CC       {ECO:0000269|PubMed:3087960}.
CC   -!- SIMILARITY: Belongs to the IucA/IucC family. {ECO:0000305}.
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DR   EMBL; X76100; CAA53709.1; -; Genomic_DNA.
DR   PIR; S50883; S50883.
DR   AlphaFoldDB; Q47318; -.
DR   SMR; Q47318; -.
DR   KEGG; ag:CAA53709; -.
DR   BioCyc; MetaCyc:MON-11596; -.
DR   BRENDA; 6.3.2.39; 2026.
DR   UniPathway; UPA00014; -.
DR   GO; GO:0050565; F:aerobactin synthase activity; IMP:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019270; P:aerobactin biosynthetic process; IMP:UniProtKB.
DR   GO; GO:0055072; P:iron ion homeostasis; IMP:UniProtKB.
DR   GO; GO:0019290; P:siderophore biosynthetic process; IEA:InterPro.
DR   InterPro; IPR007310; Aerobactin_biosyn_IucA/IucC_N.
DR   InterPro; IPR022770; FhuF_domain.
DR   InterPro; IPR037455; LucA/IucC-like.
DR   PANTHER; PTHR34384; PTHR34384; 1.
DR   Pfam; PF06276; FhuF; 1.
DR   Pfam; PF04183; IucA_IucC; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Ligase; Nucleotide-binding; Plasmid; Transferase.
FT   CHAIN           1..580
FT                   /note="Aerobactin synthase"
FT                   /id="PRO_0000415793"
SQ   SEQUENCE   580 AA;  66846 MW;  406FB857BB367F65 CRC64;
     MNHKDWDLVN RRLVAKMLSE LEYEQVFHAE SQGDDRYCIN LPGAQWRFIA ERGIWGWLWI
     DAQTLRCADE PVLAQTLLMQ LKQVLSMSDA TVAEHMQDLY ATLLGDLQLL KARRGLSASD
     LINLNADRLQ CLLSGHPKFV FNKGRRGWGK EALERYAPEY ANTFRLHWLA VKREHMIWRC
     DNEMDIHQLL TAAMDPQEFA RFSQVWQENG LDHNWLPLPV HPWQWQEKIA TDFIADFGEG
     RMVSLGEFGD QWLAQQSLRT LTNASRRGGL DIKLPLTIYN TSCYRGIPGR YIAAGPLASR
     WLQQVFATDA TLVQSGAVIL GEPAAGYVSH EGYAALARAP YRYQEMLGVI WRENPCRWLK
     PDESPFLMAT LMEWDENNQP LAGAYIDRSG LDAETWLTQL FRVVVVPLYH LLCRYGVALI
     AHGQNITLAM KEGVPQRVLL KDFQGDMRLV KEEFPEMDSL PQEVRDVTSR LSADYLIHDL
     QTGHFVTVLR FISPLMVRLG VPERRFYQLL AAVLSDYMKK HPQMSERFAL FSLFRPQIIR
     VVLNPVKLTW PDLDGGSRML PNYLEDLQNP LWLVTQEYES