ID   IUCC_VIBMI              Reviewed;         591 AA.
AC   Q76BS5;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=Aerobactin synthase;
DE            EC=6.3.2.39;
GN   Name=iucC;
OS   Vibrio mimicus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=674;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=7PT;
RX   PubMed=15215626; DOI=10.1111/j.1348-0421.2004.tb03528.x;
RA   Moon Y.H., Tanabe T., Funahashi T., Shiuchi K., Nakao H., Yamamoto S.;
RT   "Identification and characterization of two contiguous operons required for
RT   aerobactin transport and biosynthesis in Vibrio mimicus.";
RL   Microbiol. Immunol. 48:389-398(2004).
CC   -!- FUNCTION: Catalyzes the attachment of the second N-acetyl-N-
CC       hydroxylysine to the carboxylic group of N-citryl-N-acetyl-N-
CC       hydroxylysine to yield aerobactin. Involved in the biosynthesis of the
CC       siderophore aerobactin which is a chelator that mediates the high-
CC       affinity iron transport systems induced under iron-stressed conditions.
CC       {ECO:0000269|PubMed:15215626}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + N(2)-citryl-N(6)-acetyl-N(6)-hydroxy-L-lysine + N(6)-
CC         acetyl-N(6)-hydroxy-L-lysine = aerobactin + AMP + diphosphate + H(+);
CC         Xref=Rhea:RHEA:32167, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58122, ChEBI:CHEBI:58396,
CC         ChEBI:CHEBI:63796, ChEBI:CHEBI:456215; EC=6.3.2.39;
CC         Evidence={ECO:0000269|PubMed:15215626};
CC   -!- PATHWAY: Siderophore biosynthesis; aerobactin biosynthesis.
CC   -!- SIMILARITY: Belongs to the IucA/IucC family. {ECO:0000305}.
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DR   EMBL; AB110784; BAD02219.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q76BS5; -.
DR   SMR; Q76BS5; -.
DR   UniPathway; UPA00014; -.
DR   GO; GO:0016881; F:acid-amino acid ligase activity; IEA:UniProt.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019270; P:aerobactin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019290; P:siderophore biosynthetic process; IEA:InterPro.
DR   InterPro; IPR007310; Aerobactin_biosyn_IucA/IucC_N.
DR   InterPro; IPR022770; FhuF_domain.
DR   InterPro; IPR037455; LucA/IucC-like.
DR   PANTHER; PTHR34384; PTHR34384; 2.
DR   Pfam; PF06276; FhuF; 1.
DR   Pfam; PF04183; IucA_IucC; 2.
PE   1: Evidence at protein level;
KW   ATP-binding; Ligase; Nucleotide-binding; Transferase.
FT   CHAIN           1..591
FT                   /note="Aerobactin synthase"
FT                   /id="PRO_0000418721"
SQ   SEQUENCE   591 AA;  67163 MW;  5A2A3EC42624E908 CRC64;
     MVMDQLALHR YWAVANQKMV GKILSEFAYE QAFQFEPTAQ GYQLNLENGT RYCFAGEENI
     WGQVMIDPTS ITRHAEIEAD EPISAALLMR DLQPLLKMPD DAFAEHLEDL NATLLGDCKL
     MQRNEAITAR DLAMLPCEQQ QTYFDGHPKF VFNKGRRGWG SDDLKRYAPE AERVSNWVGS
     RFITRFCSSP PTMKSHGKPC CKAPSRPMKS SRWTVCWLPI SLDSTIIVMF RFILWQWSNK
     LALLFVREIA TKQLVYLGEF GDHFLPQLSL RTLSNVTRPA GYDIKLPLTV MNTSCYRGIP
     GRYILAGPTA SDWIDQVFKS DPLLIAKQAE VLQEPAAAFA AQADYALLPN APYRYHELLG
     VIWRESAASK LKAGERAILM AALMESDNQG QPLIAEYVQA SGLTLEAWLS KLFDAVVIPY
     YHLLCNYGVS LIAHGQNVTL VLENHAPKRI LLKDFQGDMR LVSREYPEQA SLDDSVKKVT
     VRLPEHLIIH DLQTGHFVTT LRFISPLVAK LGFSEPQFYR LLGDRLKAYM AAHREYQPRF
     EQFDLFKPRI LRIGLNLAKF RHSTDASASR MLPDMDDMLN NPLTKALEHQ G