ID   IWS2_ARATH              Reviewed;         406 AA.
AC   O49413;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Protein IWS1 homolog 2 {ECO:0000305};
DE            Short=AtIWS2 {ECO:0000303|PubMed:20139304};
GN   Name=IWS2 {ECO:0000303|PubMed:20139304};
GN   OrderedLocusNames=At4g19000 {ECO:0000312|Araport:AT4G19000};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=cv. En-2;
RX   PubMed=20139304; DOI=10.1073/pnas.0909198107;
RA   Li L., Ye H., Guo H., Yin Y.;
RT   "Arabidopsis IWS1 interacts with transcription factor BES1 and is involved
RT   in plant steroid hormone brassinosteroid regulated gene expression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:3918-3923(2010).
CC   -!- FUNCTION: Transcription factor involved in RNA polymerase II (RNAPII)
CC       transcription regulation. Involved in transcription elongation. May
CC       function at post-recruitment and elongation steps of transcription.
CC       {ECO:0000250|UniProtKB:F4ICK8}.
CC   -!- INTERACTION:
CC       O49413; Q9LN63: BZR2; NbExp=3; IntAct=EBI-15834301, EBI-617078;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00649}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       conditions. {ECO:0000269|PubMed:20139304}.
CC   -!- SIMILARITY: Belongs to the IWS1 family. {ECO:0000305}.
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DR   EMBL; AL021711; CAA16757.1; -; Genomic_DNA.
DR   EMBL; AL161549; CAB78902.1; -; Genomic_DNA.
DR   EMBL; CP002687; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; T05037; T05037.
DR   AlphaFoldDB; O49413; -.
DR   SMR; O49413; -.
DR   DIP; DIP-58599N; -.
DR   IntAct; O49413; 1.
DR   STRING; 3702.AT4G19000.1; -.
DR   PaxDb; O49413; -.
DR   PRIDE; O49413; -.
DR   ProteomicsDB; 238964; -.
DR   EnsemblPlants; AT4G19000.1; AT4G19000.1; AT4G19000.
DR   Gramene; AT4G19000.1; AT4G19000.1; AT4G19000.
DR   Araport; AT4G19000; -.
DR   TAIR; locus:2117199; AT4G19000.
DR   eggNOG; KOG1793; Eukaryota.
DR   HOGENOM; CLU_040584_2_1_1; -.
DR   InParanoid; O49413; -.
DR   PhylomeDB; O49413; -.
DR   PRO; PR:O49413; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; O49413; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:InterPro.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription, elongation; IEA:InterPro.
DR   Gene3D; 1.20.930.10; -; 1.
DR   InterPro; IPR044204; IWS1/2.
DR   InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR   InterPro; IPR017923; TFIIS_N.
DR   PANTHER; PTHR47350; PTHR47350; 1.
DR   Pfam; PF08711; Med26; 1.
DR   PROSITE; PS51319; TFIIS_N; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..406
FT                   /note="Protein IWS1 homolog 2"
FT                   /id="PRO_0000437493"
FT   DOMAIN          201..284
FT                   /note="TFIIS N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          41..89
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        54..89
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   406 AA;  47095 MW;  1D2463E73F500091 CRC64;
     MQELDSSNDE WVKELEGENE ESKFTGRRLV KKSISVPELV DEVEEDLDDF TEPADDFNDK
     VGKKRQRKKK DESGLEKTKK NKKQNSEEVQ EMWDSITNNT NSQYGDKVVV KPPKKKDEDA
     EEIKKLFSLR KKKSKCDKTS MEIGMQVEQV MANLEIAVED DVICNREGKP AINKLMKLPL
     LNETLSKKPL QGEFLDHGVL NLLKNWLEPL PDGSLPNINI RSAVLMILND FRIDLDQDSR
     REQLIKSGLG KVIMFLSKSD EETTPNRRLA NDIINKWGRI IYNKSTRYDN MFTQEELDEQ
     RQILLRRQTK TAPKVSGTRA RDFNTDIDLY ELGTWTGRAR AKIPTTMSMD FKIRPPSKVD
     INQEEEPCSK WQMEKRHKNK QQKNIRKGGM QALKLSVDGR TMLKYL