IX10L_ARATH
ID IX10L_ARATH Reviewed; 415 AA.
AC Q940Q8; Q8LB70; Q9FLS5; W8PUW2;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Probable beta-1,4-xylosyltransferase IRX10L;
DE EC=2.4.2.-;
DE AltName: Full=Glucuronoxylan glucuronosyltransferase 1;
DE Short=AtGUT1;
DE AltName: Full=Glucuronoxylan glucuronosyltransferase 2;
DE Short=AtGUT2;
DE AltName: Full=Protein IRREGULAR XYLEM 10-like;
DE AltName: Full=Xylan xylosyltransferase IRX10L;
GN Name=IRX10L; Synonyms=GUT1, GUT2; OrderedLocusNames=At5g61840;
GN ORFNames=MAC9.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], WEB RESOURCE, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=24905498; DOI=10.1111/tpj.12577;
RA Lao J., Oikawa A., Bromley J.R., McInerney P., Suttangkakul A.,
RA Smith-Moritz A.M., Plahar H., Chiu T.-Y., Gonzalez Fernandez-Nino S.M.G.,
RA Ebert B., Yang F., Christiansen K.M., Hansen S.F., Stonebloom S.,
RA Adams P.D., Ronald P.C., Hillson N.J., Hadi M.Z., Vega-Sanchez M.E.,
RA Loque D., Scheller H.V., Heazlewood J.L.;
RT "The plant glycosyltransferase clone collection for functional genomics.";
RL Plant J. 79:517-529(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 75-415.
RX PubMed=12451175; DOI=10.1073/pnas.252530499;
RA Iwai H., Masaoka N., Ishii T., Satoh S.;
RT "A pectin glucuronyltransferase gene is essential for intercellular
RT attachment in the plant meristem.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16319-16324(2002).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=18980649; DOI=10.1111/j.1365-313x.2008.03724.x;
RA Wu A.-M., Rihouey C., Seveno M., Hoernblad E., Singh S.K., Matsunaga T.,
RA Ishii T., Lerouge P., Marchant A.;
RT "The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for
RT glucuronoxylan biosynthesis during secondary cell wall formation.";
RL Plant J. 57:718-731(2009).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18980662; DOI=10.1111/j.1365-313x.2008.03729.x;
RA Brown D.M., Zhang Z., Stephens E., Dupree P., Turner S.R.;
RT "Characterization of IRX10 and IRX10-like reveals an essential role in
RT glucuronoxylan biosynthesis in Arabidopsis.";
RL Plant J. 57:732-746(2009).
RN [9]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=20424005; DOI=10.1104/pp.110.154971;
RA Wu A.M., Hoernblad E., Voxeur A., Gerber L., Rihouey C., Lerouge P.,
RA Marchant A.;
RT "Analysis of the Arabidopsis IRX9/IRX9-L and IRX14/IRX14-L pairs of
RT glycosyltransferase genes reveals critical contributions to biosynthesis of
RT the hemicellulose glucuronoxylan.";
RL Plant Physiol. 153:542-554(2010).
CC -!- FUNCTION: Involved in the synthesis of the hemicellulose
CC glucuronoxylan, a major component of secondary cell walls. Probably
CC involved in the elongation of glucuronoxylan xylosyl backbone.
CC {ECO:0000269|PubMed:18980649, ECO:0000269|PubMed:18980662,
CC ECO:0000269|PubMed:20424005}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q9ZUV3}; Single-pass type II membrane protein
CC {ECO:0000250|UniProtKB:Q9ZUV3}.
CC -!- TISSUE SPECIFICITY: Present in the xylem and phloem, and, to a lower
CC extent, in interfascicular cells. Expressed in the root tip, shoot
CC apical meristem (SAM), xylem cells of roots and stems, and in the
CC vasculature of roots, cotyledons and leaves.
CC {ECO:0000269|PubMed:18980649, ECO:0000269|PubMed:20424005}.
CC -!- DEVELOPMENTAL STAGE: In flowers, expressed in anthers, stigmas and
CC styles. {ECO:0000269|PubMed:18980649}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype (PubMed:18980662). Slightly
CC short inflorescence and reduced fertility (PubMed:18980649).
CC {ECO:0000269|PubMed:18980649, ECO:0000269|PubMed:18980662}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 47 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10084.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=JBEI Glycosyltransferase (GT) Collection;
CC URL="http://gt.jbei.org/arabidopsis.html";
CC -!- WEB RESOURCE: Name=CAZY, the Carbohydrate Active enZYmes database;
CC URL="http://www.cazy.org/GT64_all.html";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KJ138631; AHL38571.1; -; mRNA.
DR EMBL; AB010069; BAB10084.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97524.1; -; Genomic_DNA.
DR EMBL; AY054180; AAL06841.1; -; mRNA.
DR EMBL; AY066039; AAL47406.1; -; mRNA.
DR EMBL; AY087390; AAM64940.1; -; mRNA.
DR EMBL; AB080693; BAC20929.1; -; mRNA.
DR RefSeq; NP_568941.1; NM_125578.3.
DR AlphaFoldDB; Q940Q8; -.
DR STRING; 3702.AT5G61840.1; -.
DR CAZy; GT47; Glycosyltransferase Family 47.
DR PaxDb; Q940Q8; -.
DR PRIDE; Q940Q8; -.
DR ProteomicsDB; 232252; -.
DR EnsemblPlants; AT5G61840.1; AT5G61840.1; AT5G61840.
DR GeneID; 836306; -.
DR Gramene; AT5G61840.1; AT5G61840.1; AT5G61840.
DR KEGG; ath:AT5G61840; -.
DR Araport; AT5G61840; -.
DR TAIR; locus:2159198; AT5G61840.
DR eggNOG; KOG1021; Eukaryota.
DR HOGENOM; CLU_039682_1_0_1; -.
DR InParanoid; Q940Q8; -.
DR OMA; FHRTEHT; -.
DR OrthoDB; 789556at2759; -.
DR PhylomeDB; Q940Q8; -.
DR BioCyc; ARA:AT5G61840-MON; -.
DR PRO; PR:Q940Q8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q940Q8; baseline and differential.
DR Genevisible; Q940Q8; AT.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0005797; C:Golgi medial cisterna; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0047517; F:1,4-beta-D-xylan synthase activity; IDA:TAIR.
DR GO; GO:0080116; F:glucuronoxylan glucuronosyltransferase activity; IMP:TAIR.
DR GO; GO:0042546; P:cell wall biogenesis; IMP:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0010417; P:glucuronoxylan biosynthetic process; IMP:TAIR.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:TAIR.
DR GO; GO:0006486; P:protein glycosylation; IEA:InterPro.
DR GO; GO:0045492; P:xylan biosynthetic process; IDA:TAIR.
DR InterPro; IPR004263; Exostosin.
DR InterPro; IPR040911; Exostosin_GT47.
DR PANTHER; PTHR11062; PTHR11062; 1.
DR Pfam; PF03016; Exostosin; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycoprotein; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..415
FT /note="Probable beta-1,4-xylosyltransferase IRX10L"
FT /id="PRO_0000407577"
FT TOPO_DOM 1
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 2..22
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..415
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CONFLICT 158
FT /note="G -> R (in Ref. 5; AAM64940)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 415 AA; 47195 MW; 0A7C9350CE253A5D CRC64;
MKLSSCVLIF LLCNTFSSIS AFRLSRSQPT ERISGSAGDV LEDDPVGRLK VFVYELPSKY
NKKILQKDPR CLNHMFAAEI YMQRFLLSSP VRTLNPEEAD WFYVPVYTTC DLTPNGLPLP
FKSPRMMRSA IQLIASNWPY WNRTEGADHF FVVPHDFGAC FHYQEEKAIG RGILPLLQRA
TLVQTFGQRN HVCLKEGSIT VPPYAPPQKM QSHLIPEKTP RSIFVYFRGL FYDVGNDPEG
GYYARGARAA VWENFKDNPL FDISTEHPTT YYEDMQRAIF CLCPLGWAPW SPRLVEAVIF
GCIPVIIADD IVLPFADAIP WEDIGVFVDE KDVPYLDTIL TSIPPEVILR KQRLLANPSM
KQAMLFPQPA QPGDAFHQVL NGLARKLPHE RSVYLRPGEK LLNWTAGPVA DLKPW
