ID   IXCI_RHIMP              Reviewed;          65 AA.
AC   P83516;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2005, sequence version 2.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Chymotrypsin-elastase inhibitor ixodidin;
OS   Rhipicephalus microplus (Cattle tick) (Boophilus microplus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Rhipicephalinae;
OC   Rhipicephalus; Boophilus.
OX   NCBI_TaxID=6941;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, DISULFIDE BONDS, AND
RP   PYROGLUTAMATE FORMATION AT GLN-1.
RC   TISSUE=Hemocyte;
RX   PubMed=16191451; DOI=10.1016/j.peptides.2005.07.013;
RA   Fogaca A.C., Almeida I.C., Eberlin M.N., Tanaka A.S., Bulet P., Daffre S.;
RT   "Ixodidin, a novel antimicrobial peptide from the hemocytes of the cattle
RT   tick Boophilus microplus with inhibitory activity against serine
RT   proteinases.";
RL   Peptides 27:667-674(2006).
CC   -!- FUNCTION: Inhibits chymotrypsin and elastase. Has antibacterial
CC       activity against Gram-positive bacterium M.luteus and the Gram-negative
CC       bacterium E.coli. {ECO:0000269|PubMed:16191451}.
CC   -!- MASS SPECTROMETRY: Mass=7103; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16191451};
CC   -!- SIMILARITY: Belongs to the serine protease inhibitor-like (TIL domain-
CC       containing) family. {ECO:0000305}.
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DR   AlphaFoldDB; P83516; -.
DR   SMR; P83516; -.
DR   MEROPS; I08.051; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR036084; Ser_inhib-like_sf.
DR   InterPro; IPR002919; TIL_dom.
DR   Pfam; PF01826; TIL; 1.
DR   SUPFAM; SSF57567; SSF57567; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Protease inhibitor; Pyrrolidone carboxylic acid; Serine protease inhibitor.
FT   CHAIN           1..65
FT                   /note="Chymotrypsin-elastase inhibitor ixodidin"
FT                   /id="PRO_0000174333"
FT   DOMAIN          9..62
FT                   /note="TIL"
FT                   /evidence="ECO:0000255"
FT   SITE            36..37
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250|UniProtKB:P56682"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:16191451"
FT   DISULFID        9..42
FT                   /evidence="ECO:0000269|PubMed:16191451"
FT   DISULFID        18..38
FT                   /evidence="ECO:0000269|PubMed:16191451"
FT   DISULFID        22..34
FT                   /evidence="ECO:0000269|PubMed:16191451"
FT   DISULFID        26..62
FT                   /evidence="ECO:0000269|PubMed:16191451"
FT   DISULFID        44..56
FT                   /evidence="ECO:0000269|PubMed:16191451"
SQ   SEQUENCE   65 AA;  7129 MW;  F62B67A0EFD84C4F CRC64;
     QRGSRGQRCG PGEVFNQCGS ACPRVCGRPP AQACTLQCVS GCFCRRGYIR TQRGGCIPER
     QCHQR