ID   IXOL_IXOPA              Reviewed;          60 AA.
AC   Q6B8D2;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 27.
DE   RecName: Full=Ixodegrin-Ip {ECO:0000303|PubMed:16102420};
DE   AltName: Full=Platelet aggregation inhibitor {ECO:0000250|UniProtKB:Q4PMW1};
DE            Short=PAI {ECO:0000250|UniProtKB:Q4PMW1};
DE   AltName: Full=Tick anti-thrombosis peptide {ECO:0000250|UniProtKB:Q4PMW1};
DE   Flags: Precursor;
OS   Ixodes pacificus (Western black-legged tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX   NCBI_TaxID=29930;
RN   [1] {ECO:0000312|EMBL:AAT92147.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Salivary gland;
RX   PubMed=16102420; DOI=10.1016/j.ibmb.2005.05.007;
RA   Francischetti I.M., My Pham V., Mans B.J., Andersen J.F., Mather T.N.,
RA   Lane R.S., Ribeiro J.M.;
RT   "The transcriptome of the salivary glands of the female western black-
RT   legged tick Ixodes pacificus (Acari: Ixodidae).";
RL   Insect Biochem. Mol. Biol. 35:1142-1161(2005).
CC   -!- FUNCTION: Tick salivary platelet aggregation inhibitor that plays an
CC       important part in the anti-hemostatic strategy of ticks. Inhibits
CC       platelet aggregation induced by ADP, thrombin and thromboxane A2
CC       (TXA2). Blocks platelet adhesion to soluble collagen (most probably
CC       through the binding to alpha-2/beta-1 integrin (ITGA2/ITGB1)) and binds
CC       to purified glycoprotein IIb/IIIa (ITGA2B/ITGB3) in a dose-dependent
CC       manner. In vivo, reduces thrombus weight effectively in a rat
CC       arteriovenous shunt model and inhibits thrombosis in a carrageenan-
CC       induced mouse tail thrombosis model. {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- TISSUE SPECIFICITY: Expressed in salivary glands.
CC       {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- SIMILARITY: Belongs to the ixodegrin family. {ECO:0000305}.
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DR   EMBL; AY674214; AAT92147.1; -; mRNA.
PE   3: Inferred from homology;
KW   Disulfide bond; Hemostasis impairing toxin;
KW   Platelet aggregation inhibiting toxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..60
FT                   /note="Ixodegrin-Ip"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_5004270840"
FT   MOTIF           49..51
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000250|UniProtKB:Q4PMW1"
SQ   SEQUENCE   60 AA;  6537 MW;  01F4AC20EFD0A04A CRC64;
     MNAAFIAALF ILGALTLDAM AYSPTCEGKP CANNTDCKGS NLCQCRPPRG DDWRNFCSEY