ID   IXOP_IXOSC              Reviewed;          60 AA.
AC   B7Q052;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Ixodegrin-like peptide {ECO:0000305};
DE   AltName: Full=Platelet aggregation inhibitor {ECO:0000250|UniProtKB:Q4PMW1};
DE            Short=PAI {ECO:0000250|UniProtKB:Q4PMW1};
DE   AltName: Full=Tick anti-thrombosis peptide {ECO:0000250|UniProtKB:Q4PMW1};
DE   Flags: Precursor;
GN   ORFNames=IscW_ISCW009460 {ECO:0000312|EMBL:EEC12224.1};
OS   Ixodes scapularis (Black-legged tick) (Deer tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX   NCBI_TaxID=6945;
RN   [1] {ECO:0000312|EMBL:EEC12224.1, ECO:0000312|Proteomes:UP000001555}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Wikel;
RG   Ixodes scapularis Genome Project Consortium;
RA   Caler E., Hannick L.I., Bidwell S., Joardar V., Thiagarajan M., Amedeo P.,
RA   Galinsky K.J., Schobel S., Inman J., Hostetler J., Miller J., Hammond M.,
RA   Megy K., Lawson D., Kodira C., Sutton G., Meyer J., Hill C.A., Birren B.,
RA   Nene V., Collins F., Alarcon-Chaidez F., Wikel S., Strausberg R.;
RT   "Annotation of Ixodes scapularis.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EnsemblMetazoa:ISCW009460-PA}
RP   IDENTIFICATION.
RC   STRAIN=Wikel {ECO:0000312|EnsemblMetazoa:ISCW009460-PA};
RG   EnsemblMetazoa;
RL   Submitted (MAY-2020) to UniProtKB.
CC   -!- FUNCTION: Tick salivary platelet aggregation inhibitor that plays an
CC       important part in the anti-hemostatic strategy of ticks. Inhibits
CC       platelet aggregation induced by ADP, thrombin and thromboxane A2
CC       (TXA2). Blocks platelet adhesion to soluble collagen (most probably
CC       through the binding to alpha-2/beta-1 integrin (ITGA2/ITGB1)) and binds
CC       to purified glycoprotein IIb/IIIa (ITGA2B/ITGB3) in a dose-dependent
CC       manner. In vivo, reduces thrombus weight effectively in a rat
CC       arteriovenous shunt model and inhibits thrombosis in a carrageenan-
CC       induced mouse tail thrombosis model. {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- TISSUE SPECIFICITY: Expressed in salivary glands.
CC       {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000250|UniProtKB:Q4PMW1}.
CC   -!- SIMILARITY: Belongs to the ixodegrin family. {ECO:0000305}.
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DR   EMBL; ABJB010256642; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DS829927; EEC12224.1; -; Genomic_DNA.
DR   RefSeq; XP_002406885.1; XM_002406841.1.
DR   EnsemblMetazoa; ISCI009460-RA; ISCI009460-PA; ISCI009460.
DR   EnsemblMetazoa; ISCW009460-RA; ISCW009460-PA; ISCW009460.
DR   KEGG; isc:IscW_ISCW009460; -.
DR   VEuPathDB; VectorBase:ISCI009460; -.
DR   VEuPathDB; VectorBase:ISCW009460; -.
DR   HOGENOM; CLU_2944257_0_0_1; -.
DR   Proteomes; UP000001555; Unassembled WGS sequence.
PE   3: Inferred from homology;
KW   Disulfide bond; Hemostasis impairing toxin;
KW   Platelet aggregation inhibiting toxin; Reference proteome; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..60
FT                   /note="Ixodegrin-like peptide"
FT                   /id="PRO_5010826538"
FT   MOTIF           49..51
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000250|UniProtKB:Q4PMW1"
SQ   SEQUENCE   60 AA;  6899 MW;  67B478194B773651 CRC64;
     MNAVFIAALL ILGTSTFDAM RLWNYCTHIL CTNDSDCGQS DSCRCRPPRG DDYRYHCSRY