IXR1_YEAST
ID IXR1_YEAST Reviewed; 597 AA.
AC P33417; D6VXQ4;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Intrastrand cross-link recognition protein;
DE AltName: Full=Structure-specific recognition protein;
DE Short=SSRP;
GN Name=IXR1; Synonyms=ORD1; OrderedLocusNames=YKL032C; ORFNames=YKL245;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8342024; DOI=10.1126/science.8342024;
RA Brown S.J., Kellett P.J., Lippard S.J.;
RT "Ixr1, a yeast protein that binds to platinated DNA and confers sensitivity
RT to cisplatin.";
RL Science 261:603-605(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=8041793; DOI=10.1073/pnas.91.15.7345;
RA Lambert J.R., Bilanchone V.W., Cumsky M.G.;
RT "The ORD1 gene encodes a transcription factor involved in oxygen regulation
RT and is identical to IXR1, a gene that confers cisplatin sensitivity to
RT Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:7345-7349(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8203146; DOI=10.1002/yea.320100112;
RA Purnelle B., Skala J., van Dyck L., Goffeau A.;
RT "Analysis of an 11.7 kb DNA fragment of chromosome XI reveals a new tRNA
RT gene and four new open reading frames including a leucine zipper protein
RT and a homologue to the yeast mitochondrial regulator ABF2.";
RL Yeast 10:125-130(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-532, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Binds to platinated DNA and confers sensitivity to the
CC anticancer drug cisplatin. Activate the expression of the COX5B gene.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267,
CC ECO:0000269|PubMed:14562095}.
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DR EMBL; X71622; CAB37853.1; -; Genomic_DNA.
DR EMBL; L16900; AAA02859.1; -; Unassigned_DNA.
DR EMBL; Z28032; CAA81867.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09124.1; -; Genomic_DNA.
DR PIR; S37849; S37849.
DR RefSeq; NP_012893.3; NM_001179598.3.
DR AlphaFoldDB; P33417; -.
DR SMR; P33417; -.
DR BioGRID; 34101; 583.
DR IntAct; P33417; 52.
DR STRING; 4932.YKL032C; -.
DR iPTMnet; P33417; -.
DR MaxQB; P33417; -.
DR PaxDb; P33417; -.
DR PRIDE; P33417; -.
DR EnsemblFungi; YKL032C_mRNA; YKL032C; YKL032C.
DR GeneID; 853836; -.
DR KEGG; sce:YKL032C; -.
DR SGD; S000001515; IXR1.
DR VEuPathDB; FungiDB:YKL032C; -.
DR eggNOG; KOG0381; Eukaryota.
DR HOGENOM; CLU_032670_1_0_1; -.
DR InParanoid; P33417; -.
DR OMA; KEWESCY; -.
DR BioCyc; YEAST:G3O-31835-MON; -.
DR Reactome; R-SCE-140342; Apoptosis induced DNA fragmentation.
DR Reactome; R-SCE-163282; Mitochondrial transcription initiation.
DR PRO; PR:P33417; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P33417; protein.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0001046; F:core promoter sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0003684; F:damaged DNA binding; IDA:SGD.
DR GO; GO:0008301; F:DNA binding, bending; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0071456; P:cellular response to hypoxia; IMP:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:2000819; P:regulation of nucleotide-excision repair; IGI:SGD.
DR GO; GO:0090069; P:regulation of ribosome biogenesis; IMP:SGD.
DR Gene3D; 1.10.30.10; -; 2.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR033311; Ixr1/Abf2.
DR PANTHER; PTHR48112:SF17; PTHR48112:SF17; 1.
DR Pfam; PF00505; HMG_box; 2.
DR SMART; SM00398; HMG; 2.
DR SUPFAM; SSF47095; SSF47095; 2.
DR PROSITE; PS50118; HMG_BOX_2; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..597
FT /note="Intrastrand cross-link recognition protein"
FT /id="PRO_0000048573"
FT DNA_BIND 361..429
FT /note="HMG box 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT DNA_BIND 434..502
FT /note="HMG box 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 174..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 259..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 327..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 543..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 532
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 73
FT /note="Missing (in Ref. 1; AAA02859)"
FT /evidence="ECO:0000305"
FT CONFLICT 93
FT /note="Y -> F (in Ref. 1; AAA02859)"
FT /evidence="ECO:0000305"
FT CONFLICT 114..118
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="A -> T (in Ref. 1; AAA02859)"
FT /evidence="ECO:0000305"
FT CONFLICT 220..221
FT /note="AA -> TT (in Ref. 1; AAA02859)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 597 AA; 67856 MW; AF3B705AA2C5AC74 CRC64;
MNTGISPKQD DASNSNLLNI GQDHSLQYQG LEHNDSQYRD ASHQTPHQYL NQFQAQPQQQ
QQQQQQQQQQ QQQAPYQGHF QQSPQQQQQN VYYPLPPQSL TQPTSQSQQQ QQQQQQQQYA
NSNSNSNNNV NVNALPQDFG YMQQTGSGQN YPTINQQQFS EFYNSFLSHL TQKQTNPSVT
GTGASSNNNS NNNNVSSGNN STSSNPAQLA ASQLNPATAA AAAANNAAGP ASYLSQLPQV
QRYYPNNMNA LSSLLDPSSA GNAAGNANTA THPGLLPPNL QPQLTHHQQQ MQQQLQLQQQ
QQLQQQQQLQ QQHQLQQQQQ LQQQHHHLQQ QQQQQQHPVV KKLSSTQSRI ERRKQLKKQG
PKRPSSAYFL FSMSIRNELL QQFPEAKVPE LSKLASARWK ELTDDQKKPF YEEFRTNWEK
YRVVRDAYEK TLPPKRPSGP FIQFTQEIRP TVVKENPDKG LIEITKIIGE RWRELDPAKK
AEYTETYKKR LKEWESCYPD ENDPNGNPTG HSHKAMNMNL NMDTKIMENQ DSIEHITANA
IDSVTGSNSN STNPNTPVSP PISLQQQPLQ QQQQQQQQQQ HMLLADPTTN GSIIKNE
