ID   4OT_PSEUF               Reviewed;          63 AA.
AC   P49172;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=2-hydroxymuconate tautomerase;
DE            EC=5.3.2.6;
DE   AltName: Full=4-oxalocrotonate tautomerase;
DE            Short=4-OT;
GN   Name=dmpI;
OS   Pseudomonas sp. (strain CF600).
OG   Plasmid pVI150.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=79676;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1732207; DOI=10.1128/jb.174.3.711-724.1992;
RA   Shingler V., Marklund U., Powlowski J.;
RT   "Nucleotide sequence and functional analysis of the complete phenol/3,4-
RT   dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600.";
RL   J. Bacteriol. 174:711-724(1992).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
RX   PubMed=8547259; DOI=10.1021/bi951732k;
RA   Subramanya H.S., Roper D.I., Dauter Z., Dodson E.J., Davies G.J.,
RA   Wilson K.S., Wigley D.B.;
RT   "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism
RT   investigated by the crystal structures of two isomerases.";
RL   Biochemistry 35:792-802(1996).
CC   -!- FUNCTION: Catalyzes the ketonization of 2-hydroxymuconate
CC       stereoselectively to yield 2-oxo-3-hexenedioate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-
CC         enedioate; Xref=Rhea:RHEA:33431, ChEBI:CHEBI:28080,
CC         ChEBI:CHEBI:64908; EC=5.3.2.6;
CC   -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC   -!- SUBUNIT: Homohexamer.
CC   -!- SIMILARITY: Belongs to the 4-oxalocrotonate tautomerase family.
CC       {ECO:0000305}.
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DR   EMBL; X60835; CAA43229.1; -; Genomic_DNA.
DR   PDB; 1OTF; X-ray; 1.90 A; A/B/C/D/E/F=2-63.
DR   PDBsum; 1OTF; -.
DR   AlphaFoldDB; P49172; -.
DR   SMR; P49172; -.
DR   BRENDA; 5.3.2.6; 5085.
DR   UniPathway; UPA00273; -.
DR   EvolutionaryTrace; P49172; -.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00491; 4Oxalocrotonate_Tautomerase; 1.
DR   Gene3D; 3.30.429.10; -; 1.
DR   InterPro; IPR004370; 4-oxalocrotonate_tautomerase.
DR   InterPro; IPR014347; Tautomerase/MIF_sf.
DR   InterPro; IPR018191; Tautomerase_Pseudo-typ.
DR   Pfam; PF01361; Tautomerase; 1.
DR   SUPFAM; SSF55331; SSF55331; 1.
DR   TIGRFAMs; TIGR00013; taut; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aromatic hydrocarbons catabolism; Isomerase; Plasmid.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..63
FT                   /note="2-hydroxymuconate tautomerase"
FT                   /id="PRO_0000209513"
FT   ACT_SITE        2
FT                   /note="Proton acceptor; via imino nitrogen"
FT                   /evidence="ECO:0000250"
FT   STRAND          3..10
FT                   /evidence="ECO:0007829|PDB:1OTF"
FT   HELIX           14..32
FT                   /evidence="ECO:0007829|PDB:1OTF"
FT   HELIX           36..38
FT                   /evidence="ECO:0007829|PDB:1OTF"
FT   STRAND          40..46
FT                   /evidence="ECO:0007829|PDB:1OTF"
FT   HELIX           48..50
FT                   /evidence="ECO:0007829|PDB:1OTF"
FT   STRAND          51..53
FT                   /evidence="ECO:0007829|PDB:1OTF"
SQ   SEQUENCE   63 AA;  7105 MW;  CEBC26092D1E5342 CRC64;
     MPIAQLYIIE GRTDEQKETL IRQVSEAMAN SLDAPLERVR VLITEMPKNH FGIGGEPASK
     VRR