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IYD_DROME
ID   IYD_DROME               Reviewed;         287 AA.
AC   E1JIB2;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Iodotyrosine deiodinase {ECO:0000312|FlyBase:FBgn0286980};
DE            EC=1.21.1.1 {ECO:0000269|PubMed:27643701};
DE   AltName: Full=Halotyrosine dehalogenase {ECO:0000305|PubMed:27643701};
DE   AltName: Full=Protein condet {ECO:0000303|PubMed:27643701};
GN   Name=Iyd {ECO:0000312|FlyBase:FBgn0286980};
GN   Synonyms=cdt {ECO:0000303|PubMed:27643701};
GN   ORFNames=CG46438 {ECO:0000312|FlyBase:FBgn0286980};
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN   [1] {ECO:0000312|Proteomes:UP000000803}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2] {ECO:0000312|Proteomes:UP000000803}
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3] {ECO:0000305}
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, AND
RP   MUTAGENESIS OF GLU-154; TYR-158 AND LYS-179.
RX   PubMed=27643701; DOI=10.1002/pro.3044;
RA   Phatarphekar A., Rokita S.E.;
RT   "Functional analysis of iodotyrosine deiodinase from drosophila
RT   melanogaster.";
RL   Protein Sci. 25:2187-2195(2016).
RN   [4] {ECO:0000305}
RP   FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP   GLU-154.
RX   PubMed=29764939; DOI=10.1074/jbc.ra118.003364;
RA   Phatarphekar A., Su Q., Eun S.H., Chen X., Rokita S.E.;
RT   "The importance of a halotyrosine dehalogenase for Drosophila fertility.";
RL   J. Biol. Chem. 293:10314-10321(2018).
CC   -!- FUNCTION: Catalyzes the dehalogenation of halotyrosines such as 3-
CC       bromo-L-tyrosine, 3-chloro-L-tyrosine, 3-iodo-L-tyrosine and 3,5-
CC       diiodo-L-tyrosine (PubMed:27643701). Activity towards 3-fluoro-L-
CC       tyrosine is weak (PubMed:27643701). Important for male and female
CC       fertility (PubMed:29764939). May be involved in maintaining the
CC       viability of sperm, both during development in the testes and storage
CC       in the female spermatheca (PubMed:29764939).
CC       {ECO:0000269|PubMed:27643701, ECO:0000269|PubMed:29764939}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 iodide + L-tyrosine + 2 NADP(+) = 3,5-diiodo-L-tyrosine +
CC         H(+) + 2 NADPH; Xref=Rhea:RHEA:32479, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16382, ChEBI:CHEBI:57506, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58315, ChEBI:CHEBI:58349; EC=1.21.1.1;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:32481;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=iodide + L-tyrosine + NADP(+) = 3-iodo-L-tyrosine + NADPH;
CC         Xref=Rhea:RHEA:27453, ChEBI:CHEBI:16382, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58315, ChEBI:CHEBI:58349, ChEBI:CHEBI:59898;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:27455;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-iodo-L-tyrosine + iodide + NADP(+) = 3,5-diiodo-L-tyrosine +
CC         H(+) + NADPH; Xref=Rhea:RHEA:27457, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16382, ChEBI:CHEBI:57506, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:59898;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:27459;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chloride + L-tyrosine + NADP(+) = 3-chloro-L-tyrosine + NADPH;
CC         Xref=Rhea:RHEA:70343, ChEBI:CHEBI:17996, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58315, ChEBI:CHEBI:58349, ChEBI:CHEBI:189422;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:70345;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=bromide + L-tyrosine + NADP(+) = 3-bromo-L-tyrosine + NADPH;
CC         Xref=Rhea:RHEA:70347, ChEBI:CHEBI:15858, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58315, ChEBI:CHEBI:58349, ChEBI:CHEBI:189423;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:70349;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000269|PubMed:27643701};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=14 uM for 3-iodo-L-tyrosine (at pH 7.4 and 25 degrees Celsius)
CC         {ECO:0000269|PubMed:27643701};
CC         KM=8 uM for 3-bromo-L-tyrosine (at pH 7.4 and 25 degrees Celsius)
CC         {ECO:0000269|PubMed:27643701};
CC         KM=21 uM for 3-chloro-L-tyrosine (at pH 7.4 and 25 degrees Celsius)
CC         {ECO:0000269|PubMed:27643701};
CC         KM=11 uM for 3,5-diiodo-L-tyrosine (at pH 7.4 and 25 degrees Celsius)
CC         {ECO:0000269|PubMed:27643701};
CC         Note=kcat is 12 sec(-1) for the dehalogenation of 3-iodo-L-tyrosine
CC         (at pH 7.4 and 25 degrees Celsius) (PubMed:27643701). kcat is 6.9
CC         sec(-1) for the dehalogenation of 3-bromo-L-tyrosine (at pH 7.4 and
CC         25 degrees Celsius) (PubMed:27643701). kcat is 5.0 sec(-1) for the
CC         dehalogenation of 3-chloro-L-tyrosine (at pH 7.4 and 25 degrees
CC         Celsius) (PubMed:27643701). kcat is 10.8 sec(-1) for the
CC         dehalogenation of 3,5-diiodo-L-tyrosine (at pH 7.4 and 25 degrees
CC         Celsius) (PubMed:27643701). {ECO:0000269|PubMed:27643701};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q6PHW0}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in spermatocytes.
CC       {ECO:0000269|PubMed:29764939}.
CC   -!- DISRUPTION PHENOTYPE: Adults produce fewer progeny (PubMed:29764939).
CC       Larvae display an increase in survival when fed a diet enriched with 3-
CC       chloro-L-tyrosine (Cl-Tyr), whereas survival is unaffected when fed a
CC       diet containing 3-iodo-L-tyrosine (I-Tyr) or 3-bromo-L-tyrosine (Br-
CC       Tyr) (PubMed:29764939). Adult survival is unaffected when fed a diet
CC       supplemented with halotyrosines (PubMed:29764939).
CC       {ECO:0000269|PubMed:29764939}.
CC   -!- MISCELLANEOUS: Named condet in honor of Dr. Jean Francois Condet who
CC       discovered that ingestion of iodide could reduce goiter.
CC       {ECO:0000269|PubMed:27643701}.
CC   -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR   EMBL; AE014296; ACZ94685.1; -; Genomic_DNA.
DR   EMBL; AE014296; QJC18614.1; -; Genomic_DNA.
DR   RefSeq; NP_001163414.1; NM_001169943.1.
DR   SMR; E1JIB2; -.
DR   PRIDE; E1JIB2; -.
DR   EnsemblMetazoa; FBtr0475275; FBpp0423399; FBgn0286980.
DR   EnsemblMetazoa; FBtr0475279; FBpp0423404; FBgn0286980.
DR   GeneID; 39242; -.
DR   KEGG; dme:Dmel_CG46438; -.
DR   CTD; 389434; -.
DR   FlyBase; FBgn0286980; Iyd.
DR   VEuPathDB; VectorBase:FBgn0286980; -.
DR   GeneTree; ENSGT00390000004348; -.
DR   HOGENOM; CLU_403487_0_0_1; -.
DR   OMA; WVDQDLQ; -.
DR   OrthoDB; 887662at2759; -.
DR   BRENDA; 1.21.1.1; 1994.
DR   BioGRID-ORCS; 39242; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 39242; -.
DR   Proteomes; UP000000803; Chromosome 3L.
DR   Genevisible; E1JIB2; DM.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0010181; F:FMN binding; IDA:UniProtKB.
DR   GO; GO:0004447; F:iodide peroxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0140616; F:iodotyrosine deiodinase activity; IDA:FlyBase.
DR   GO; GO:1905516; P:positive regulation of fertilization; IMP:UniProtKB.
DR   Gene3D; 3.40.109.10; -; 1.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   SUPFAM; SSF55469; SSF55469; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Flavoprotein; FMN; Membrane; NADP; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..287
FT                   /note="Iodotyrosine deiodinase"
FT                   /id="PRO_0000455636"
FT   TRANSMEM        15..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         96..100
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHW0"
FT   BINDING         125..126
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q9DCX8"
FT   BINDING         125
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHW0"
FT   BINDING         127
FT                   /ligand="3,5-diiodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:57506"
FT                   /evidence="ECO:0000250|UniProtKB:Q9DCX8"
FT   BINDING         127
FT                   /ligand="3-iodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:59898"
FT                   /evidence="ECO:0000250|UniProtKB:Q9DCX8"
FT   BINDING         154
FT                   /ligand="3,5-diiodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:57506"
FT                   /evidence="ECO:0000269|PubMed:27643701"
FT   BINDING         154
FT                   /ligand="3-iodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:59898"
FT                   /evidence="ECO:0000269|PubMed:27643701"
FT   BINDING         158
FT                   /ligand="3,5-diiodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:57506"
FT                   /evidence="ECO:0000269|PubMed:27643701"
FT   BINDING         158
FT                   /ligand="3-iodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:59898"
FT                   /evidence="ECO:0000269|PubMed:27643701"
FT   BINDING         179
FT                   /ligand="3,5-diiodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:57506"
FT                   /evidence="ECO:0000269|PubMed:27643701"
FT   BINDING         179
FT                   /ligand="3-iodo-L-tyrosine"
FT                   /ligand_id="ChEBI:CHEBI:59898"
FT                   /evidence="ECO:0000269|PubMed:27643701"
FT   BINDING         235..237
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHW0"
FT   BINDING         277
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000250|UniProtKB:Q6PHW0"
FT   MUTAGEN         154
FT                   /note="E->Q: Significant decrease in catalytic efficiency
FT                   and reduced affinity for substrates 3-iodo-L-tyrosine (I-
FT                   Tyr) and 3,5-diiodo-L-tyrosine (I2-Tyr). Larvae survival is
FT                   decreased when fed a diet supplemented with 100 or 200um of
FT                   I-Tyr but is increased when fed a diet enriched with 3-
FT                   chloro-L-tyrosine (Cl-Tyr). However, adult survival is
FT                   unaffected when fed a diet supplemented with
FT                   halotyrosines."
FT                   /evidence="ECO:0000269|PubMed:27643701,
FT                   ECO:0000269|PubMed:29764939"
FT   MUTAGEN         158
FT                   /note="Y->F: Moderate decrease in catalytic efficiency and
FT                   reduced affinity for substrates I-Tyr and I2-Tyr."
FT                   /evidence="ECO:0000269|PubMed:27643701"
FT   MUTAGEN         179
FT                   /note="K->Q: Moderate decrease in catalytic activity but
FT                   strongly reduces affinity for substrates I-Tyr and I2-Tyr."
FT                   /evidence="ECO:0000269|PubMed:27643701"
SQ   SEQUENCE   287 AA;  32597 MW;  D7128DE1D178EC7C CRC64;
     MDVDELISSS KLLKHWPSLF ITLALIWIVK RLFFKGNRVL KTYNLDEQVE EEVEHFADLG
     DELQPALEDK PHVPFVPGQN LNPNGAKRLY ELMRGRRSIR SFNSHPKPDL SVIEDCIRAA
     GTAPSGAHTE PWTYCVVQEP ELKRSIREIV EQEELVNYSQ RMHPQWVTDL RPLQTNHVKE
     YLTEAPYLIL IFKQTYGLSE NGKRMRRHYY NEISTSIAAG ILLCALQAAG LASLVTTPLN
     CGPALRNLLG RPVNEKLLIL LPVGYPKDGC TVPDLARKNL SNIMVTF
 
 
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