IYO_ARATH
ID IYO_ARATH Reviewed; 1465 AA.
AC Q8GYU3; Q9SVE1; Q9SZM5;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Transcriptional elongation regulator MINIYO {ECO:0000303|PubMed:21620701};
GN Name=IYO {ECO:0000303|PubMed:21620701};
GN Synonyms=MINIYO {ECO:0000303|PubMed:21620701};
GN OrderedLocusNames=At4g38440 {ECO:0000312|Araport:AT4G38440};
GN ORFNames=F20M13.10 {ECO:0000312|EMBL:CAB37501.1},
GN F22I13.210 {ECO:0000312|EMBL:BAC42056.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, MUTAGENESIS OF GLY-962, DISRUPTION PHENOTYPE, INTERACTION WITH
RP HAG3; NRPB3 AND NRPB10L, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=21620701; DOI=10.1016/j.cub.2011.04.041;
RA Sanmartin M., Sauer M., Munoz A., Zouhar J., Ordonez A., van de Ven W.T.,
RA Caro E., de la Paz Sanchez M., Raikhel N.V., Gutierrez C.,
RA Sanchez-Serrano J.J., Rojo E.;
RT "A molecular switch for initiating cell differentiation in Arabidopsis.";
RL Curr. Biol. 21:999-1008(2011).
RN [6]
RP REVIEW.
RX PubMed=22456317; DOI=10.4161/trns.3.1.19303;
RA Sanmartin M., Sauer M., Munoz A., Rojo E.;
RT "MINIYO and transcriptional elongation: lifting the roadblock to
RT differentiation.";
RL Transcription 3:25-28(2012).
CC -!- FUNCTION: Positive regulator of transcriptional elongation that is
CC essential for cells to initiate differentiation. Interacts with RNA
CC polymerase II and the Elongator complex and is required to sustain
CC global levels of transcriptional elongation activity, specifically in
CC differentiating tissues. {ECO:0000269|PubMed:21620701}.
CC -!- SUBUNIT: Interacts with HAG3, NRPB3 and NRPB10L.
CC {ECO:0000269|PubMed:21620701}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21620701}. Nucleus
CC {ECO:0000269|PubMed:21620701}. Note=Cytoplasmic in undifferentiated
CC cells (meristem core) and nuclear in differentiating cells.
CC {ECO:0000269|PubMed:21620701}.
CC -!- TISSUE SPECIFICITY: Expressed in root and shoot apices and in leaf and
CC flower primordia. Detected in the endosperm, embryo, meristems and in
CC organ primordia, but not in mature cells. Found exclusively in the
CC vascular bundles in mature leaves. {ECO:0000269|PubMed:21620701}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype when heterozygous. Embryo
CC lethal when homozygous. {ECO:0000269|PubMed:21620701}.
CC -!- MISCELLANEOUS: Over-expression of IYO activates premature cell
CC differentiation and confers resistance to the elongation inhibitor 6-
CC azauracil. {ECO:0000269|PubMed:21620701}.
CC -!- SIMILARITY: Belongs to the RPAP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB37500.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB37501.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80509.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL035539; CAB37500.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL035540; CAB37501.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161593; CAB80509.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86929.1; -; Genomic_DNA.
DR EMBL; BT005439; AAO63859.1; -; mRNA.
DR EMBL; AK117387; BAC42056.1; -; mRNA.
DR PIR; T05672; T05672.
DR PIR; T05673; T05673.
DR RefSeq; NP_195557.2; NM_120006.3.
DR AlphaFoldDB; Q8GYU3; -.
DR SMR; Q8GYU3; -.
DR IntAct; Q8GYU3; 1.
DR STRING; 3702.AT4G38440.1; -.
DR PaxDb; Q8GYU3; -.
DR PRIDE; Q8GYU3; -.
DR ProteomicsDB; 238966; -.
DR EnsemblPlants; AT4G38440.1; AT4G38440.1; AT4G38440.
DR GeneID; 830001; -.
DR Gramene; AT4G38440.1; AT4G38440.1; AT4G38440.
DR KEGG; ath:AT4G38440; -.
DR Araport; AT4G38440; -.
DR TAIR; locus:2121758; AT4G38440.
DR eggNOG; KOG4732; Eukaryota.
DR HOGENOM; CLU_002899_1_0_1; -.
DR InParanoid; Q8GYU3; -.
DR OMA; CYWKYSA; -.
DR OrthoDB; 25908at2759; -.
DR PhylomeDB; Q8GYU3; -.
DR PRO; PR:Q8GYU3; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8GYU3; baseline and differential.
DR Genevisible; Q8GYU3; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0030154; P:cell differentiation; IMP:TAIR.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR013929; RNA_pol_II_AP1_C.
DR InterPro; IPR013930; RNA_pol_II_AP1_N.
DR Pfam; PF08620; RPAP1_C; 1.
DR Pfam; PF08621; RPAP1_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Differentiation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1465
FT /note="Transcriptional elongation regulator MINIYO"
FT /id="PRO_0000433607"
FT REGION 27..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 186..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1113..1135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1117..1131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 962
FT /note="G->E: In iyo-1; delayed onset of cell
FT differentiation in all meristems, but no effect on the
FT binding to NRPB3 and NRPB10L."
FT /evidence="ECO:0000269|PubMed:21620701"
SQ SEQUENCE 1465 AA; 162425 MW; A8077C75D08A8AC6 CRC64;
MEQSSGRVNP EQPNNVLASL VGSIVEKGIS ENKPPSKPLP PRPSLLSFPV ARHRSHGPHL
APVGSSIAQP KDYNDDQEEE EAEERFMNAD SIAAFAKPLQ RKEKKDMDLG RWKDMVSGDD
PASTHVPQQS RKLKIIETRP PYVASADAAT TSSNTLLAAR ASDQREFVSD KAPFIKNLGT
KERVPLNASP PLAVSNGLGT RHASSSLESD IDVENHAKLQ TMSPDEIAEA QAELLDKMDP
ALLSILKKRG EAKLKKRKHS VQGVSITDET AKNSRTEGHF VTPKVMAIPK EKSVVQKPGI
AQGFVWDAWT ERVEAARDLR FSFDGNVVEE DVVSPAETGG KWSGVESAAE RDFLRTEGDP
GAAGYTIKEA IALARSVIPG QRCLALHLLA SVLDKALNKL CQSRIGYARE EKDKSTDWEA
IWAYALGPEP ELVLALRMAL DDNHASVVIA CVKVIQCLLS CSLNENFFNI LENMGPHGKD
IFTASVFRSK PEIDLGFLRG CYWKYSAKPS NIVAFREEIL DDGTEDTDTI QKDVFVAGQD
VAAGLVRMDI LPRIYHLLET EPTAALEDSI ISVTIAIARH SPKCTTAILK YPKFVQTIVK
RFQLNKRMDV LSSQINSVRL LKVLARYDQS TCMEFVKNGT FNAVTWHLFQ FTSSLDSWVK
LGKQNCKLSS TLMVEQLRFW KVCIHSGCCV SRFPELFPAL CLWLSCPSFE KLREKNLISE
FTSVSNEAYL VLEAFAETLP NMYSQNIPRN ESGTWDWSYV SPMIDSALSW ITLAPQLLKW
EKGIESVSVS TTTLLWLYSG VMRTISKVLE KISAEGEEEP LPWLPEFVPK IGLAIIKHKL
LSFSVADVSR FGKDSSRCSS FMEYLCFLRE RSQDDELALA SVNCLHGLTR TIVSIQNLIE
SARSKMKAPH QVSISTGDES VLANGILAES LAELTSVSCS FRDSVSSEWP IVQSIELHKR
GGLAPGVGLG WGASGGGFWS TRVLLAQAGA GLLSLFLNIS LSDSQNDQGS VGFMDKVNSA
LAMCLIAGPR DYLLVERAFE YVLRPHALEH LACCIKSNKK NISFEWECSE GDYHRMSSML
ASHFRHRWLQ QKGRSIAEEG VSGVRKGTVG LETIHEDGEM SNSSTQDKKS DSSTIEWAHQ
RMPLPPHWFL SAISAVHSGK TSTGPPESTE LLEVAKAGVF FLAGLESSSG FGSLPSPVVS
VPLVWKFHAL STVLLVGMDI IEDKNTRNLY NYLQELYGQF LDEARLNHRD TELLRFKSDI
HENYSTFLEM VVEQYAAVSY GDVVYGRQVS VYLHQCVEHS VRLSAWTVLS NARVLELLPS
LDKCLGEADG YLEPVEENEA VLEAYLKSWT CGALDRAATR GSVAYTLVVH HFSSLVFCNQ
AKDKVSLRNK IVKTLVRDLS RKRHREGMML DLLRYKKGSA NAMEEEVIAA ETEKRMEVLK
EGCEGNSTLL LELEKLKSAA LCGRR
