IZUM2_MOUSE
ID IZUM2_MOUSE Reviewed; 220 AA.
AC Q9DA16; Q0P5Z2;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Izumo sperm-egg fusion protein 2;
DE Flags: Precursor;
GN Name=Izumo2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 10-220 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19658160; DOI=10.1002/mrd.21092;
RA Ellerman D.A., Pei J., Gupta S., Snell W.J., Myles D., Primakoff P.;
RT "Izumo is part of a multiprotein family whose members form large complexes
RT on mammalian sperm.";
RL Mol. Reprod. Dev. 76:1188-1199(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9DA16-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9DA16-2; Sequence=VSP_037225;
CC Name=3;
CC IsoId=Q9DA16-3; Sequence=VSP_037224, VSP_037226;
CC -!- MISCELLANEOUS: Izumo is the name of a Japanese shrine to marriage.
CC -!- SIMILARITY: Belongs to the Izumo family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24492.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC150895; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK006268; BAB24492.1; ALT_INIT; mRNA.
DR EMBL; BC048526; AAH48526.1; -; mRNA.
DR CCDS; CCDS52235.1; -. [Q9DA16-2]
DR RefSeq; NP_083593.1; NM_029317.1. [Q9DA16-2]
DR RefSeq; XP_006541301.1; XM_006541238.2.
DR AlphaFoldDB; Q9DA16; -.
DR SMR; Q9DA16; -.
DR BioGRID; 217536; 1.
DR PhosphoSitePlus; Q9DA16; -.
DR PRIDE; Q9DA16; -.
DR ProteomicsDB; 269351; -. [Q9DA16-1]
DR ProteomicsDB; 269352; -. [Q9DA16-2]
DR ProteomicsDB; 269353; -. [Q9DA16-3]
DR TopDownProteomics; Q9DA16-1; -. [Q9DA16-1]
DR Antibodypedia; 49261; 29 antibodies from 12 providers.
DR Ensembl; ENSMUST00000085422; ENSMUSP00000082543; ENSMUSG00000066500. [Q9DA16-2]
DR GeneID; 75510; -.
DR KEGG; mmu:75510; -.
DR UCSC; uc009gqk.2; mouse. [Q9DA16-2]
DR CTD; 126123; -.
DR MGI; MGI:1922760; Izumo2.
DR VEuPathDB; HostDB:ENSMUSG00000066500; -.
DR GeneTree; ENSGT00390000015068; -.
DR HOGENOM; CLU_104457_0_0_1; -.
DR InParanoid; Q9DA16; -.
DR OMA; AVFMFMV; -.
DR OrthoDB; 1528398at2759; -.
DR PhylomeDB; Q9DA16; -.
DR TreeFam; TF337020; -.
DR Reactome; R-MMU-1300645; Acrosome Reaction and Sperm:Oocyte Membrane Binding.
DR BioGRID-ORCS; 75510; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q9DA16; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9DA16; protein.
DR Bgee; ENSMUSG00000066500; Expressed in spermatid and 5 other tissues.
DR ExpressionAtlas; Q9DA16; baseline and differential.
DR Genevisible; Q9DA16; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR029389; IZUMO.
DR InterPro; IPR042920; IZUMO2.
DR PANTHER; PTHR47745; PTHR47745; 1.
DR Pfam; PF15005; IZUMO; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..220
FT /note="Izumo sperm-egg fusion protein 2"
FT /id="PRO_0000373774"
FT TOPO_DOM 21..185
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..220
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..129
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_037224"
FT VAR_SEQ 139..166
FT /note="HSFKAEVLDCLYCKKTMPKCVKKKYCFE -> Q (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_037225"
FT VAR_SEQ 208..212
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_037226"
FT CONFLICT 10
FT /note="L -> R (in Ref. 2; BAB24492)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 220 AA; 24868 MW; 3C78758E7D1857AB CRC64;
MPLALALVLL CGLGGPGAWG CLQCDQSVLL ELRQLRDAIV TKRFHLEGLQ ARAQALLLSM
EGPFFRDYAM NAFVGKVVVD QLEKVATSFK NQAQYIKANS KTDVPLLEEL VSFREHAIKE
LKGALRDYEM KACDHKTCHS FKAEVLDCLY CKKTMPKCVK KKYCFEDGQF RMTLKFQDDN
KPRNMVLVGD LVTVGLAILT FLVILIAACT YRQNRKLLLK
