IZUM3_MOUSE
ID IZUM3_MOUSE Reviewed; 263 AA.
AC A6PWV3; Q9DAG1;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Izumo sperm-egg fusion protein 3;
DE Flags: Precursor;
GN Name=Izumo3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBUNIT, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19658160; DOI=10.1002/mrd.21092;
RA Ellerman D.A., Pei J., Gupta S., Snell W.J., Myles D., Primakoff P.;
RT "Izumo is part of a multiprotein family whose members form large complexes
RT on mammalian sperm.";
RL Mol. Reprod. Dev. 76:1188-1199(2009).
CC -!- SUBUNIT: Monomer and homodimer. {ECO:0000269|PubMed:19658160}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A6PWV3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A6PWV3-2; Sequence=VSP_034458;
CC -!- TISSUE SPECIFICITY: Sperm-specific (at protein level).
CC {ECO:0000269|PubMed:19658160}.
CC -!- MISCELLANEOUS: Izumo is the name of a Japanese shrine to marriage.
CC -!- SIMILARITY: Belongs to the Izumo family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BC099579; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK005867; BAB24287.1; -; mRNA.
DR EMBL; AL807382; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC099579; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS51231.1; -. [A6PWV3-1]
DR RefSeq; NP_081310.1; NM_027034.1. [A6PWV3-1]
DR AlphaFoldDB; A6PWV3; -.
DR STRING; 10090.ENSMUSP00000102725; -.
DR GlyGen; A6PWV3; 2 sites.
DR PaxDb; A6PWV3; -.
DR PRIDE; A6PWV3; -.
DR ProteomicsDB; 269354; -. [A6PWV3-1]
DR Antibodypedia; 49567; 1 antibodies from 1 providers.
DR Ensembl; ENSMUST00000107108; ENSMUSP00000102725; ENSMUSG00000028533. [A6PWV3-1]
DR GeneID; 69314; -.
DR KEGG; mmu:69314; -.
DR UCSC; uc012dha.1; mouse. [A6PWV3-1]
DR CTD; 100129669; -.
DR MGI; MGI:1916564; Izumo3.
DR VEuPathDB; HostDB:ENSMUSG00000028533; -.
DR eggNOG; ENOG502S8ID; Eukaryota.
DR GeneTree; ENSGT00390000017601; -.
DR HOGENOM; CLU_103188_0_0_1; -.
DR InParanoid; A6PWV3; -.
DR OMA; SEDCIVT; -.
DR OrthoDB; 1563154at2759; -.
DR PhylomeDB; A6PWV3; -.
DR TreeFam; TF339468; -.
DR Reactome; R-MMU-1300645; Acrosome Reaction and Sperm:Oocyte Membrane Binding.
DR BioGRID-ORCS; 69314; 3 hits in 70 CRISPR screens.
DR PRO; PR:A6PWV3; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A6PWV3; protein.
DR Bgee; ENSMUSG00000028533; Expressed in spermatid and 11 other tissues.
DR ExpressionAtlas; A6PWV3; baseline and differential.
DR Genevisible; A6PWV3; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR InterPro; IPR029389; IZUMO.
DR Pfam; PF15005; IZUMO; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..263
FT /note="Izumo sperm-egg fusion protein 3"
FT /id="PRO_0000342458"
FT TOPO_DOM 23..176
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 241..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 98
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 128
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..153
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_034458"
FT CONFLICT 214
FT /note="L -> M (in Ref. 1; BAB24287)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 30314 MW; A1851EDA83E82D6B CRC64;
MGDLWVLLFS SLSLAAFHGV RGCLECDPKF TEDVRTLLGN LVPLEVPDRN QLLDRQHKEI
THISSKVSHK DKMLRLLAVR NVIKLREWLK DEFYRLGNET WKGVFIIQGN LLEIRQNLEK
KLTQIIKNFS EVACSEDCIV IEGPVLDCWN CLRMTTRCFK GDYCQDEDPK TAENREISLY
LIFIAEAVIL ASAVLLFHVC ISHRRKMKAI RRTLKTYLEK KLEELVEMIY KDDGEKNLEF
GRSNSNSLTG EPTCAESEMQ TGT
