ID   IZUM4_MOUSE             Reviewed;         227 AA.
AC   D3Z690; Q6PEP3;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Izumo sperm-egg fusion protein 4;
DE   Flags: Precursor;
GN   Name=Izumo4;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=129; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=D3Z690-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=D3Z690-2; Sequence=VSP_042504, VSP_042505;
CC   -!- MISCELLANEOUS: Izumo is the name of a Japanese shrine to marriage.
CC   -!- SIMILARITY: Belongs to the Izumo family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC152410; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466553; EDL31509.1; -; Genomic_DNA.
DR   EMBL; BC057953; AAH57953.1; -; mRNA.
DR   CCDS; CCDS35983.1; -. [D3Z690-1]
DR   RefSeq; NP_082105.3; NM_027829.3. [D3Z690-1]
DR   AlphaFoldDB; D3Z690; -.
DR   STRING; 10090.ENSMUSP00000093076; -.
DR   GlyGen; D3Z690; 2 sites.
DR   PhosphoSitePlus; D3Z690; -.
DR   PaxDb; D3Z690; -.
DR   PRIDE; D3Z690; -.
DR   ProteomicsDB; 269232; -. [D3Z690-1]
DR   ProteomicsDB; 269233; -. [D3Z690-2]
DR   Antibodypedia; 57175; 61 antibodies from 16 providers.
DR   DNASU; 71564; -.
DR   Ensembl; ENSMUST00000218184; ENSMUSP00000151397; ENSMUSG00000055862. [D3Z690-1]
DR   GeneID; 71564; -.
DR   KEGG; mmu:71564; -.
DR   UCSC; uc007gej.1; mouse. [D3Z690-1]
DR   CTD; 113177; -.
DR   MGI; MGI:1918814; Izumo4.
DR   VEuPathDB; HostDB:ENSMUSG00000055862; -.
DR   eggNOG; ENOG502SNMJ; Eukaryota.
DR   GeneTree; ENSGT00390000015418; -.
DR   HOGENOM; CLU_110285_0_0_1; -.
DR   InParanoid; D3Z690; -.
DR   OMA; LHCHNNF; -.
DR   OrthoDB; 1110467at2759; -.
DR   PhylomeDB; D3Z690; -.
DR   TreeFam; TF338561; -.
DR   Reactome; R-MMU-1300645; Acrosome Reaction and Sperm:Oocyte Membrane Binding.
DR   BioGRID-ORCS; 71564; 1 hit in 73 CRISPR screens.
DR   PRO; PR:D3Z690; -.
DR   Proteomes; UP000000589; Chromosome 10.
DR   RNAct; D3Z690; protein.
DR   Bgee; ENSMUSG00000055862; Expressed in spermatid and 176 other tissues.
DR   ExpressionAtlas; D3Z690; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR029389; IZUMO.
DR   Pfam; PF15005; IZUMO; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..227
FT                   /note="Izumo sperm-egg fusion protein 4"
FT                   /id="PRO_0000416109"
FT   CARBOHYD        153
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        214
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..141
FT                   /note="MFGQGRLGQAMALLLFLGMTAALARGCLQCDPNFAEKFSFYRHHVNLKSWWV
FT                   GDIPVSGMLLSDWIQNTMKELHLAIPAEITRKKLYQVAEAVYQRMDQLYQGKMYFPGYF
FT                   PNELRAIFREQVRLIQNAIIESRIDCQRHC -> MVQAKGHSGSGPGWGGPRGVWGCFS
FT                   YIA (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_042504"
FT   VAR_SEQ         195..227
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_042505"
SQ   SEQUENCE   227 AA;  26332 MW;  27505406B1A71444 CRC64;
     MFGQGRLGQA MALLLFLGMT AALARGCLQC DPNFAEKFSF YRHHVNLKSW WVGDIPVSGM
     LLSDWIQNTM KELHLAIPAE ITRKKLYQVA EAVYQRMDQL YQGKMYFPGY FPNELRAIFR
     EQVRLIQNAI IESRIDCQRH CGIYQYETIS CSNCTDSHVI CFGYYCKSSA QWESAVQGLL
     KYINTWHKQD EKMRTTPAFL SQNIKCLEPQ HLVNLTLEKV SECLTQH