JAC1_ARATH
ID JAC1_ARATH Reviewed; 651 AA.
AC Q9C9Q4;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 140.
DE RecName: Full=J domain-containing protein required for chloroplast accumulation response 1;
GN Name=JAC1; OrderedLocusNames=At1g75100; ORFNames=F9E10.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN CHLOROPLAST MOVEMENTS, DISRUPTION
RP PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=16113208; DOI=10.1104/pp.105.067371;
RA Suetsugu N., Kagawa T., Wada M.;
RT "An auxilin-like J-domain protein, JAC1, regulates phototropin-mediated
RT chloroplast movement in Arabidopsis.";
RL Plant Physiol. 139:151-162(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP REVIEW.
RX PubMed=17696776; DOI=10.1515/bc.2007.118;
RA Suetsugu N., Wada M.;
RT "Chloroplast photorelocation movement mediated by phototropin family
RT proteins in green plants.";
RL Biol. Chem. 388:927-935(2007).
RN [6]
RP FUNCTION IN ALUMINUM RESISTANCE.
RX PubMed=17150990; DOI=10.1093/jxb/erl221;
RA Ezaki B., Kiyohara H., Matsumoto H., Nakashima S.;
RT "Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in
RT roots of Arabidopsis.";
RL J. Exp. Bot. 58:497-506(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [8]
RP FUNCTION IN ACTIN FILAMENTS REGULATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia GL1;
RX PubMed=21737483; DOI=10.1093/pcp/pcr087;
RA Ichikawa S., Yamada N., Suetsugu N., Wada M., Kadota A.;
RT "Red light, Phot1 and JAC1 modulate Phot2-dependent reorganization of
RT chloroplast actin filaments and chloroplast avoidance movement.";
RL Plant Cell Physiol. 52:1422-1432(2011).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 551-651, FUNCTION, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF 614-HIS--ASP-616.
RX PubMed=20562448; DOI=10.1093/pcp/pcq089;
RA Takano A., Suetsugu N., Wada M., Kohda D.;
RT "Crystallographic and functional analyses of J-domain of JAC1 essential for
RT chloroplast photorelocation movement in Arabidopsis thaliana.";
RL Plant Cell Physiol. 51:1372-1376(2010).
CC -!- FUNCTION: Required for chloroplast photorelocation movement;
CC chloroplast accumulation upon low blue light and for chloroplast
CC movement to the bottom of cells in darkness, by modulating chloroplast
CC actin (Cp-actin) filaments distribution, appearance and disappearance.
CC May mediate a slight resistance to aluminum in root hair cells.
CC {ECO:0000269|PubMed:16113208, ECO:0000269|PubMed:17150990,
CC ECO:0000269|PubMed:20562448, ECO:0000269|PubMed:21737483}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16113208}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and stems, but not in roots.
CC {ECO:0000269|PubMed:16113208}.
CC -!- DOMAIN: The J domain co-chaperone activity is required for chloroplast
CC photorelocation movement. {ECO:0000269|PubMed:20562448}.
CC -!- DISRUPTION PHENOTYPE: Lacks the chloroplast accumulation response under
CC weak blue light and chloroplast movement in darkness, but shows a
CC normal avoidance response under strong blue light. Non biased
CC distribution of chloroplast actin (Cp-actin) filaments.
CC {ECO:0000269|PubMed:16113208, ECO:0000269|PubMed:20562448,
CC ECO:0000269|PubMed:21737483}.
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DR EMBL; AB158477; BAE44203.1; -; mRNA.
DR EMBL; AC013258; AAG51921.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35672.1; -; Genomic_DNA.
DR EMBL; AY057504; AAL09745.1; -; mRNA.
DR EMBL; AY103303; AAM65355.1; -; mRNA.
DR PIR; A96781; A96781.
DR RefSeq; NP_565101.1; NM_106166.4.
DR PDB; 3AG7; X-ray; 1.80 A; A=551-651.
DR PDBsum; 3AG7; -.
DR AlphaFoldDB; Q9C9Q4; -.
DR SMR; Q9C9Q4; -.
DR BioGRID; 29066; 3.
DR IntAct; Q9C9Q4; 2.
DR STRING; 3702.AT1G75100.1; -.
DR iPTMnet; Q9C9Q4; -.
DR PaxDb; Q9C9Q4; -.
DR PRIDE; Q9C9Q4; -.
DR ProteomicsDB; 238968; -.
DR EnsemblPlants; AT1G75100.1; AT1G75100.1; AT1G75100.
DR GeneID; 843847; -.
DR Gramene; AT1G75100.1; AT1G75100.1; AT1G75100.
DR KEGG; ath:AT1G75100; -.
DR Araport; AT1G75100; -.
DR TAIR; locus:2037256; AT1G75100.
DR eggNOG; KOG0431; Eukaryota.
DR HOGENOM; CLU_026675_0_0_1; -.
DR InParanoid; Q9C9Q4; -.
DR OMA; WNGFNEK; -.
DR OrthoDB; 319959at2759; -.
DR PhylomeDB; Q9C9Q4; -.
DR EvolutionaryTrace; Q9C9Q4; -.
DR PRO; PR:Q9C9Q4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9Q4; baseline and differential.
DR Genevisible; Q9C9Q4; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0031982; C:vesicle; IBA:GO_Central.
DR GO; GO:0030276; F:clathrin binding; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; IMP:UniProtKB.
DR GO; GO:0071483; P:cellular response to blue light; IMP:UniProtKB.
DR GO; GO:0009904; P:chloroplast accumulation movement; IMP:TAIR.
DR GO; GO:0009903; P:chloroplast avoidance movement; IMP:UniProtKB.
DR GO; GO:0072318; P:clathrin coat disassembly; IBA:GO_Central.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IBA:GO_Central.
DR GO; GO:0080183; P:response to photooxidative stress; IEP:TAIR.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR036869; J_dom_sf.
DR SUPFAM; SSF46565; SSF46565; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..651
FT /note="J domain-containing protein required for chloroplast
FT accumulation response 1"
FT /id="PRO_0000422083"
FT DOMAIN 586..651
FT /note="J"
FT REGION 1..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 156..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 308..526
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 532..562
FT /evidence="ECO:0000255"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 333..380
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 381..401
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..483
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 484..498
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..526
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT MUTAGEN 614..616
FT /note="HPD->AAA: Impaired chloroplast photorelocation
FT movement."
FT /evidence="ECO:0000269|PubMed:20562448"
FT HELIX 551..564
FT /evidence="ECO:0007829|PDB:3AG7"
FT TURN 565..569
FT /evidence="ECO:0007829|PDB:3AG7"
FT HELIX 571..575
FT /evidence="ECO:0007829|PDB:3AG7"
FT HELIX 576..580
FT /evidence="ECO:0007829|PDB:3AG7"
FT HELIX 593..595
FT /evidence="ECO:0007829|PDB:3AG7"
FT HELIX 599..613
FT /evidence="ECO:0007829|PDB:3AG7"
FT HELIX 615..620
FT /evidence="ECO:0007829|PDB:3AG7"
FT HELIX 625..645
FT /evidence="ECO:0007829|PDB:3AG7"
FT TURN 646..648
FT /evidence="ECO:0007829|PDB:3AG7"
SQ SEQUENCE 651 AA; 71602 MW; 1FC2139336D27EEC CRC64;
MQTLPSSETV LLGSNSAPPV LRSPGGDDVD IDFGDVFGGP PKRRSKVTSN EVTRHSFSES
ALRRRDVIVD VGDLLPQDEK PVFGEDTSSV RRRFTTDDFF DDIFRVNESS SLPGSRILSP
AHKPESSSGT SSPSQFSLPA KATEIPTFNL AATRSLNKNK ETVSSSPLSR TSSKADVVST
AKSYSDDCDD PPQVFVTGKG RQFHFSIYKW PNKGVPVVIW GSSRLSSMSK AEETTPVPLS
DYRKTSVVEK LGKNEEGDGK SGLSGLKDVK KTSLKRPGVQ TKEEKTETDL KSEQAFFGVS
KAREANVKPL DSVESEQAFS GVSKAHEATT VKPLHSIFHE EDERQDEKIV SEREVRKGKS
KAKNTRSFTE DSRTKKKSQG TKSSLDSSPI PDKSSFASSS AAPEVGKDGV KGKVSDFVKI
FSKGASVGAG GESLGQSSRW RAKETPKTDI IHDGSNAKET VNIPDQQKKS TPDIPAMNRD
QKPSQSTQKK DSDRESMNYK APGDTVQEER QEPSTTHTTS EDIDEPFHVN FDVEDITQDE
NKMEEANKDA EEIKNIDAKI RKWSSGKSGN IRSLLSTLQY ILWSGSGWKP VPLMDMIEGN
AVRKSYQRAL LILHPDKLQQ KGASANQKYM AEKVFELLQE AWDHFNTLGP V
