JAL1_ARATH
ID JAL1_ARATH Reviewed; 174 AA.
AC Q9SE37; Q94K20;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Protein RESTRICTED TEV MOVEMENT 1;
DE AltName: Full=Jacalin-related lectin 1;
DE AltName: Full=Restricted tobacco etch virus movement protein 1;
GN Name=RTM1; Synonyms=JAL1; OrderedLocusNames=At1g05760; ORFNames=T20M3.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF SER-56; GLY-132
RP AND ASP-139, CHARACTERIZATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=10618445; DOI=10.1073/pnas.97.1.489;
RA Chisholm S.T., Mahajan S.K., Whitham S.A., Yamamoto M.L., Carrington J.C.;
RT "Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-
RT distance movement of tobacco etch virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:489-494(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=cv. Bla-1, cv. Columbia, cv. Di-2, cv. Ga-0, cv. Gy-0, cv. Mrk-0,
RC cv. REN-1, cv. Sorbo, cv. St-0, cv. Ta-0, cv. Wassilewskija, and cv. Wt-1;
RX PubMed=20478894; DOI=10.1098/rstb.2010.0044;
RA Lalic J., Agudelo-Romero P., Carrasco P., Elena S.F.;
RT "Adaptation of tobacco etch potyvirus to a susceptible ecotype of
RT Arabidopsis thaliana capacitates it for systemic infection of resistant
RT ecotypes.";
RL Philos. Trans. R. Soc. Lond., B, Biol. Sci. 365:1997-2007(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=cv. Akita, cv. Alc-0, cv. Blh-1, cv. Bur-0, cv. Can-0, cv. Cvi-0,
RC cv. Edi-0, cv. Ge-0, cv. Ge-1, cv. Gre-0, cv. Ita-0, cv. Jea, cv. Ll-0,
RC cv. Mh-1, cv. Mt-0, cv. N13 Konchezero, cv. Nd-1, cv. Oy-0, cv. Pyl-1,
RC cv. Sakata, cv. Sha, cv. St-0, cv. Stw-0, cv. Tsu-0, cv. Wassilewskija-2,
RC and cv. Wu-0; TISSUE=Leaf;
RX PubMed=22723957; DOI=10.1371/journal.pone.0039169;
RA Cosson P., Schurdi-Levraud V., Le Q.H., Sicard O., Caballero M., Roux F.,
RA Le Gall O., Candresse T., Revers F.;
RT "The RTM resistance to potyviruses in Arabidopsis thaliana: natural
RT variation of the RTM genes and evidence for the implication of additional
RT genes.";
RL PLoS ONE 7:E39169-E39169(2012).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=9628015; DOI=10.1046/j.1365-313x.1998.00105.x;
RA Mahajan S.K., Chisholm S.T., Whitham S.A., Carrington J.C.;
RT "Identification and characterization of a locus (RTM1) that restricts long-
RT distance movement of tobacco etch virus in Arabidopsis thaliana.";
RL Plant J. 14:177-186(1998).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9892709; DOI=10.1073/pnas.96.2.772;
RA Whitham S.A., Yamamoto M.L., Carrington J.C.;
RT "Selectable viruses and altered susceptibility mutants in Arabidopsis
RT thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:772-777(1999).
RN [9]
RP FUNCTION.
RX PubMed=10760245; DOI=10.2307/3871070;
RA Whitham S.A., Anderberg R.J., Chisholm S.T., Carrington J.C.;
RT "Arabidopsis RTM2 gene is necessary for specific restriction of tobacco
RT etch virus and encodes an unusual small heat shock-like protein.";
RL Plant Cell 12:569-582(2000).
RN [10]
RP SUBCELLULAR LOCATION, SUBUNIT, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=11743111; DOI=10.1104/pp.010479;
RA Chisholm S.T., Parra M.A., Anderberg R.J., Carrington J.C.;
RT "Arabidopsis RTM1 and RTM2 genes function in phloem to restrict long-
RT distance movement of tobacco etch virus.";
RL Plant Physiol. 127:1667-1675(2001).
RN [11]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18467340; DOI=10.1093/pcp/pcn075;
RA Nagano A.J., Fukao Y., Fujiwara M., Nishimura M., Hara-Nishimura I.;
RT "Antagonistic jacalin-related lectins regulate the size of ER body-type
RT beta-glucosidase complexes in Arabidopsis thaliana.";
RL Plant Cell Physiol. 49:969-980(2008).
RN [12]
RP INTERACTION WITH RTM3.
RX PubMed=20584941; DOI=10.1104/pp.110.155754;
RA Cosson P., Sofer L., Le Q.H., Leger V., Schurdi-Levraud V., Whitham S.A.,
RA Yamamoto M.L., Gopalan S., Le Gall O., Candresse T., Carrington J.C.,
RA Revers F.;
RT "RTM3, which controls long-distance movement of potyviruses, is a member of
RT a new plant gene family encoding a meprin and TRAF homology domain-
RT containing protein.";
RL Plant Physiol. 154:222-232(2010).
RN [13]
RP INDUCTION BY NAC045 AND NAC086, AND TISSUE SPECIFICITY.
RX PubMed=25081480; DOI=10.1126/science.1253736;
RA Furuta K.M., Yadav S.R., Lehesranta S., Belevich I., Miyashima S.,
RA Heo J.O., Vaten A., Lindgren O., De Rybel B., Van Isterdael G.,
RA Somervuo P., Lichtenberger R., Rocha R., Thitamadee S., Taehtiharju S.,
RA Auvinen P., Beeckman T., Jokitalo E., Helariutta Y.;
RT "Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis
RT culminating in enucleation.";
RL Science 345:933-937(2014).
CC -!- FUNCTION: Required for the restriction of long-distance movement of the
CC pathogenic tobacco etch virus (TEV) without causing a hypersensitive
CC response or inducing systemic acquired resistance.
CC {ECO:0000269|PubMed:10618445, ECO:0000269|PubMed:10760245,
CC ECO:0000269|PubMed:20478894, ECO:0000269|PubMed:22723957,
CC ECO:0000269|PubMed:9628015, ECO:0000269|PubMed:9892709}.
CC -!- SUBUNIT: Self-interacts. Interacts with RTM3.
CC {ECO:0000269|PubMed:11743111, ECO:0000269|PubMed:20584941}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11743111}.
CC Note=Soluble protein present in sieve elements in a punctate pattern of
CC 1 to 2 um spheres.
CC -!- TISSUE SPECIFICITY: Expressed at low levels exclusively in phloem-
CC associated cells (e.g. sieve elements and adjacent cells).
CC {ECO:0000269|PubMed:11743111, ECO:0000269|PubMed:25081480}.
CC -!- INDUCTION: Regulated by the transcription factors NAC045 and NAC086.
CC {ECO:0000269|PubMed:25081480}.
CC -!- DISRUPTION PHENOTYPE: Susceptible to systemic infection by tobacco etch
CC virus (TEV). {ECO:0000269|PubMed:10618445, ECO:0000269|PubMed:9892709}.
CC -!- SIMILARITY: Belongs to the jacalin lectin family. {ECO:0000255|PROSITE-
CC ProRule:PRU01088, ECO:0000305}.
CC -!- CAUTION: Has been shown to be inactive in cv. Ler-2 (AC D9UBI3) and cv.
CC Bl-1, cv. C24 and cv. Ct-1 (AC D9UBG0) due to naturally occurring
CC sequence variation in these strains. The sequence shown is from strain
CC cv. Columbia. {ECO:0000305}.
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DR EMBL; AF191302; AAF14583.1; -; Genomic_DNA.
DR EMBL; GU396156; ADE43047.1; -; Genomic_DNA.
DR EMBL; GU396157; ADE43048.1; -; Genomic_DNA.
DR EMBL; GU396159; ADE43050.1; -; Genomic_DNA.
DR EMBL; GU396160; ADE43051.1; -; Genomic_DNA.
DR EMBL; GU396161; ADE43052.1; -; Genomic_DNA.
DR EMBL; GU396163; ADE43054.1; -; Genomic_DNA.
DR EMBL; GU396164; ADE43055.1; -; Genomic_DNA.
DR EMBL; GU396165; ADE43056.1; -; Genomic_DNA.
DR EMBL; GU396166; ADE43057.1; -; Genomic_DNA.
DR EMBL; GU396167; ADE43058.1; -; Genomic_DNA.
DR EMBL; GU396168; ADE43059.1; -; Genomic_DNA.
DR EMBL; GU396169; ADE43060.1; -; Genomic_DNA.
DR EMBL; GU396171; ADE43062.1; -; Genomic_DNA.
DR EMBL; GU396172; ADE43063.1; -; Genomic_DNA.
DR EMBL; GU396173; ADE43064.1; -; Genomic_DNA.
DR EMBL; GU396174; ADE43065.1; -; Genomic_DNA.
DR EMBL; GU396177; ADE43067.1; -; Genomic_DNA.
DR EMBL; GU396178; ADE43068.1; -; Genomic_DNA.
DR EMBL; GU396179; ADE43069.1; -; Genomic_DNA.
DR EMBL; GU396180; ADE43070.1; -; Genomic_DNA.
DR EMBL; GU396181; ADE43071.1; -; Genomic_DNA.
DR EMBL; GU396182; ADE43072.1; -; Genomic_DNA.
DR EMBL; FR681968; CBW45825.1; -; Genomic_DNA.
DR EMBL; FR681969; CBW45826.1; -; Genomic_DNA.
DR EMBL; FR681971; CBW45828.1; -; Genomic_DNA.
DR EMBL; FR681973; CBW45830.1; -; Genomic_DNA.
DR EMBL; FR681974; CBW45831.1; -; Genomic_DNA.
DR EMBL; FR681975; CBW45832.1; -; Genomic_DNA.
DR EMBL; FR681976; CBW45833.1; -; Genomic_DNA.
DR EMBL; FR681977; CBW45834.1; -; Genomic_DNA.
DR EMBL; FR681978; CBW45835.1; -; Genomic_DNA.
DR EMBL; FR681980; CBW45837.1; -; Genomic_DNA.
DR EMBL; FR681981; CBW45838.1; -; Genomic_DNA.
DR EMBL; FR681982; CBW45839.1; -; Genomic_DNA.
DR EMBL; FR681983; CBW45840.1; -; Genomic_DNA.
DR EMBL; FR681984; CBW45841.1; -; Genomic_DNA.
DR EMBL; FR681985; CBW45842.1; -; Genomic_DNA.
DR EMBL; FR681986; CBW45843.1; -; Genomic_DNA.
DR EMBL; FR681987; CBW45844.1; -; Genomic_DNA.
DR EMBL; FR681988; CBW45845.1; -; Genomic_DNA.
DR EMBL; FR681989; CBW45846.1; -; Genomic_DNA.
DR EMBL; FR681990; CBW45847.1; -; Genomic_DNA.
DR EMBL; FR681991; CBW45848.1; -; Genomic_DNA.
DR EMBL; FR681992; CBW45849.1; -; Genomic_DNA.
DR EMBL; FR681995; CBW45852.1; -; Genomic_DNA.
DR EMBL; FR681996; CBW45853.1; -; Genomic_DNA.
DR EMBL; FR681997; CBW45854.1; -; Genomic_DNA.
DR EMBL; FR681998; CBW45855.1; -; Genomic_DNA.
DR EMBL; AC009999; AAF29382.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27888.1; -; Genomic_DNA.
DR EMBL; AF370465; AAK43842.1; -; mRNA.
DR EMBL; AY064681; AAL47386.1; -; mRNA.
DR PIR; A86192; A86192.
DR RefSeq; NP_172067.1; NM_100456.3.
DR AlphaFoldDB; Q9SE37; -.
DR SMR; Q9SE37; -.
DR BioGRID; 22326; 2.
DR IntAct; Q9SE37; 1.
DR STRING; 3702.AT1G05760.1; -.
DR PaxDb; Q9SE37; -.
DR ProteomicsDB; 232286; -.
DR EnsemblPlants; AT1G05760.1; AT1G05760.1; AT1G05760.
DR GeneID; 837084; -.
DR Gramene; AT1G05760.1; AT1G05760.1; AT1G05760.
DR KEGG; ath:AT1G05760; -.
DR Araport; AT1G05760; -.
DR TAIR; locus:2198683; AT1G05760.
DR eggNOG; ENOG502S91T; Eukaryota.
DR HOGENOM; CLU_076205_1_0_1; -.
DR InParanoid; Q9SE37; -.
DR PhylomeDB; Q9SE37; -.
DR PRO; PR:Q9SE37; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SE37; differential.
DR Genevisible; Q9SE37; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0051607; P:defense response to virus; IMP:UniProtKB.
DR GO; GO:0009615; P:response to virus; IMP:TAIR.
DR GO; GO:0046741; P:transport of virus in host, tissue to tissue; IDA:UniProtKB.
DR CDD; cd09612; Jacalin; 1.
DR Gene3D; 2.100.10.30; -; 1.
DR InterPro; IPR001229; Jacalin-like_lectin_dom.
DR InterPro; IPR033734; Jacalin-like_lectin_dom_plant.
DR InterPro; IPR036404; Jacalin-like_lectin_dom_sf.
DR Pfam; PF01419; Jacalin; 1.
DR SMART; SM00915; Jacalin; 1.
DR SUPFAM; SSF51101; SSF51101; 1.
DR PROSITE; PS51752; JACALIN_LECTIN; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Lectin; Plant defense; Reference proteome.
FT CHAIN 1..174
FT /note="Protein RESTRICTED TEV MOVEMENT 1"
FT /id="PRO_0000429162"
FT DOMAIN 1..152
FT /note="Jacalin-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT MUTAGEN 56
FT /note="S->F: In rtm1-2; susceptible to systemic infection
FT by tobacco etch virus (TEV)."
FT /evidence="ECO:0000269|PubMed:10618445"
FT MUTAGEN 132
FT /note="G->D: In rtm1-5; susceptible to systemic infection
FT by tobacco etch virus (TEV)."
FT /evidence="ECO:0000269|PubMed:10618445"
FT MUTAGEN 139
FT /note="D->N: In rtm1-1; susceptible to systemic infection
FT by tobacco etch virus (TEV)."
FT /evidence="ECO:0000269|PubMed:10618445"
FT CONFLICT 20
FT /note="D -> G (in Ref. 6; AAK43842/AAL47386)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 174 AA; 19200 MW; F38A420E5EDF0224 CRC64;
MKIGPVGKHD ARSTTIVNWD EGSHDGFISQ IFLSHGVAGI MSIQFQFVMD GKLVLSDRHG
PFSGNMFDVI ELNYPHEYIT GISGEYYKYE ANNPHMRSLK FNTNTSEYGP FGTSGSSNDK
FAFKLGKSPQ FGGFHGTYDA SGLQYIGVYL RPKTVLPKID TGNAEETESK IVLG
