JAL28_ARATH
ID JAL28_ARATH Reviewed; 470 AA.
AC Q9SDM9; B9DHB6; O04317; Q0WNW3; Q8LB83;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 2.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Nitrile-specifier protein 1;
DE Short=AtNSP1;
DE AltName: Full=Jacalin-related lectin 28;
GN Name=NSP1; Synonyms=JAL28; OrderedLocusNames=At3g16400;
GN ORFNames=MDC8.2, T02O04.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Nziengui-Ikapi H., da Silva Conceicao A., Marty F.;
RT "Arabidopsis thaliana MBP-like genes.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18467340; DOI=10.1093/pcp/pcn075;
RA Nagano A.J., Fukao Y., Fujiwara M., Nishimura M., Hara-Nishimura I.;
RT "Antagonistic jacalin-related lectins regulate the size of ER body-type
RT beta-glucosidase complexes in Arabidopsis thaliana.";
RL Plant Cell Physiol. 49:969-980(2008).
RN [10]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18987211; DOI=10.1104/pp.108.130732;
RA Burow M., Losansky A., Muller R., Plock A., Kliebenstein D.J.,
RA Wittstock U.;
RT "The genetic basis of constitutive and herbivore-induced ESP-independent
RT nitrile formation in Arabidopsis.";
RL Plant Physiol. 149:561-574(2009).
CC -!- FUNCTION: Responsible for constitutive and herbivore-induced simple
CC nitrile formation. Promotes simple nitriles, but not epithionitrile or
CC thiocyanate formation. Converts allylglucosinolate, 4-
CC methylsulfinylbutylglucosinolate, 4-methylthiobutylglucosinolate and
CC benzylglucosinolate to their corresponding simple nitriles in the
CC presence of myrosinase. {ECO:0000269|PubMed:18987211}.
CC -!- INDUCTION: By herbivory. {ECO:0000269|PubMed:18987211}.
CC -!- DISRUPTION PHENOTYPE: No simple nitriles produced.
CC {ECO:0000269|PubMed:18987211}.
CC -!- MISCELLANEOUS: Not dependent on the presence of Fe(2+) or Fe(3+).
CC -!- SIMILARITY: Belongs to the jacalin lectin family. {ECO:0000255|PROSITE-
CC ProRule:PRU01088, ECO:0000305}.
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DR EMBL; AF214574; AAF25385.1; -; mRNA.
DR EMBL; AC001645; AAB63638.1; -; Genomic_DNA.
DR EMBL; AP000373; BAB01138.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75807.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75808.1; -; Genomic_DNA.
DR EMBL; AF375399; AAK52983.1; -; mRNA.
DR EMBL; AY035036; AAK59541.1; -; mRNA.
DR EMBL; AY113857; AAM44905.1; -; mRNA.
DR EMBL; AY125507; AAM78099.1; -; mRNA.
DR EMBL; AY129466; AAM91052.1; -; mRNA.
DR EMBL; BT000689; AAN31834.1; -; mRNA.
DR EMBL; BT000690; AAN31835.1; -; mRNA.
DR EMBL; AY087364; AAM64914.1; -; mRNA.
DR EMBL; AK229323; BAF01186.1; -; mRNA.
DR EMBL; AK317467; BAH20133.1; -; mRNA.
DR RefSeq; NP_001030709.1; NM_001035632.1.
DR RefSeq; NP_566546.1; NM_112511.4.
DR PDB; 5GQT; X-ray; 3.02 A; A=1-470.
DR PDBsum; 5GQT; -.
DR AlphaFoldDB; Q9SDM9; -.
DR SMR; Q9SDM9; -.
DR BioGRID; 6221; 1.
DR IntAct; Q9SDM9; 1.
DR STRING; 3702.AT3G16400.1; -.
DR iPTMnet; Q9SDM9; -.
DR PaxDb; Q9SDM9; -.
DR PRIDE; Q9SDM9; -.
DR ProteomicsDB; 238971; -.
DR EnsemblPlants; AT3G16400.1; AT3G16400.1; AT3G16400.
DR EnsemblPlants; AT3G16400.2; AT3G16400.2; AT3G16400.
DR GeneID; 820887; -.
DR Gramene; AT3G16400.1; AT3G16400.1; AT3G16400.
DR Gramene; AT3G16400.2; AT3G16400.2; AT3G16400.
DR KEGG; ath:AT3G16400; -.
DR Araport; AT3G16400; -.
DR TAIR; locus:2088304; AT3G16400.
DR eggNOG; KOG0379; Eukaryota.
DR HOGENOM; CLU_030461_2_0_1; -.
DR InParanoid; Q9SDM9; -.
DR OMA; WKQCATP; -.
DR OrthoDB; 933937at2759; -.
DR PhylomeDB; Q9SDM9; -.
DR PRO; PR:Q9SDM9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SDM9; baseline and differential.
DR Genevisible; Q9SDM9; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; HDA:TAIR.
DR GO; GO:0019762; P:glucosinolate catabolic process; IDA:TAIR.
DR GO; GO:0080028; P:nitrile biosynthetic process; IDA:TAIR.
DR GO; GO:0080027; P:response to herbivore; IMP:TAIR.
DR CDD; cd09612; Jacalin; 1.
DR Gene3D; 2.100.10.30; -; 1.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR001229; Jacalin-like_lectin_dom.
DR InterPro; IPR033734; Jacalin-like_lectin_dom_plant.
DR InterPro; IPR036404; Jacalin-like_lectin_dom_sf.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR035429; NSP1/2/3.
DR Pfam; PF01419; Jacalin; 1.
DR Pfam; PF01344; Kelch_1; 2.
DR PIRSF; PIRSF038118; Myrosinase-db_jacalin; 1.
DR SMART; SM00915; Jacalin; 1.
DR SMART; SM00612; Kelch; 3.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF51101; SSF51101; 1.
DR PROSITE; PS51752; JACALIN_LECTIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Kelch repeat; Lectin; Reference proteome; Repeat.
FT CHAIN 1..470
FT /note="Nitrile-specifier protein 1"
FT /id="PRO_0000072797"
FT DOMAIN 2..143
FT /note="Jacalin-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT REPEAT 47..93
FT /note="Kelch 1"
FT REPEAT 177..225
FT /note="Kelch 2"
FT REPEAT 230..276
FT /note="Kelch 3"
FT REPEAT 280..329
FT /note="Kelch 4"
FT REPEAT 331..375
FT /note="Kelch 5"
FT CONFLICT 25
FT /note="R -> K (in Ref. 1; AAF25385)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="V -> I (in Ref. 1; AAF25385)"
FT /evidence="ECO:0000305"
FT CONFLICT 147
FT /note="I -> T (in Ref. 1; AAF25385 and 6; AAM64914)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="Y -> C (in Ref. 7; BAF01186)"
FT /evidence="ECO:0000305"
FT CONFLICT 376
FT /note="I -> L (in Ref. 1; AAF25385 and 6; AAM64914)"
FT /evidence="ECO:0000305"
FT STRAND 13..17
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 22..31
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 33..45
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 63..67
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 74..82
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 91..99
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 112..118
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 123..141
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 152..156
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 170..174
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 177..181
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 184..186
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 195..199
FT /evidence="ECO:0007829|PDB:5GQT"
FT TURN 200..203
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 204..207
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 220..227
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 230..237
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 246..250
FT /evidence="ECO:0007829|PDB:5GQT"
FT TURN 251..254
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 255..260
FT /evidence="ECO:0007829|PDB:5GQT"
FT TURN 262..264
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 269..271
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 273..276
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 278..284
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 287..290
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 296..300
FT /evidence="ECO:0007829|PDB:5GQT"
FT TURN 301..304
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 305..308
FT /evidence="ECO:0007829|PDB:5GQT"
FT HELIX 313..315
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 319..321
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 323..326
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 328..349
FT /evidence="ECO:0007829|PDB:5GQT"
FT TURN 350..353
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 354..357
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 361..363
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 372..376
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 379..388
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 399..408
FT /evidence="ECO:0007829|PDB:5GQT"
FT TURN 409..411
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 413..416
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 420..422
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 434..439
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 444..449
FT /evidence="ECO:0007829|PDB:5GQT"
FT STRAND 462..466
FT /evidence="ECO:0007829|PDB:5GQT"
SQ SEQUENCE 470 AA; 51670 MW; A9527780362F0157 CRC64;
MAQKLEAKGG EMGDVWDDGV YENVRKVYVG QAQYGIAFVK FEYVNGSQVV VGDEHGKKTE
LGVEEFEIDA DDYIVYVEGY REKVNDMTSE MITFLSIKTF KGKTSHPIEK RPGVKFVLHG
GKIVGFHGRS TDVLHSLGAY VSLSSTIKLL GKWIKVEQKG EGPGLRCSHG IAQVGNKIYS
FGGEFTPNQP IDKHLYVFDL ETRTWSISPA TGDVPHLSCL GVRMVSVGST LYVFGGRDAS
RQYNGFYSFD TTTNEWKLLT PVEEGPTPRS FHSMAADEEN VYVFGGVSAT ARLNTLDSYN
IVDKKWFHCS TPGDSLTARG GAGLEVVQGK VWVVYGFNGC EVDDVHYYDP VQDKWTQVET
FGVRPSERSV FASAAIGKHI VIFGGEIAMD PLAHVGPGQL TDGTFALDTE TLQWERLDKF
GGEEETPSSR GWTASTTATI DGKKGLVMHG GKAPTNDRFD DLFFYGIDSA
