JAL29_ARATH
ID JAL29_ARATH Reviewed; 619 AA.
AC O04316;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Nitrile-specifier protein 4;
DE Short=AtNSP4;
DE AltName: Full=Jacalin-related lectin 29;
GN Name=NSP4; Synonyms=JAL29; OrderedLocusNames=At3g16410;
GN ORFNames=T02O04.10, T2O4.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18467340; DOI=10.1093/pcp/pcn075;
RA Nagano A.J., Fukao Y., Fujiwara M., Nishimura M., Hara-Nishimura I.;
RT "Antagonistic jacalin-related lectins regulate the size of ER body-type
RT beta-glucosidase complexes in Arabidopsis thaliana.";
RL Plant Cell Physiol. 49:969-980(2008).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18987211; DOI=10.1104/pp.108.130732;
RA Burow M., Losansky A., Muller R., Plock A., Kliebenstein D.J.,
RA Wittstock U.;
RT "The genetic basis of constitutive and herbivore-induced ESP-independent
RT nitrile formation in Arabidopsis.";
RL Plant Physiol. 149:561-574(2009).
CC -!- FUNCTION: Promotes simple nitriles, but not epithionitrile or
CC thiocyanate formation. Converts allylglucosinolate and
CC benzylglucosinolate to their corresponding simple nitriles in the
CC presence of myrosinase. {ECO:0000269|PubMed:18987211}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:18987211}.
CC -!- MISCELLANEOUS: Contains a second mannose-binding lectin domain not
CC present in other members of the family.
CC -!- SIMILARITY: Belongs to the jacalin lectin family. {ECO:0000255|PROSITE-
CC ProRule:PRU01088, ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC001645; AAB63637.1; -; Genomic_DNA.
DR EMBL; AP000373; BAB01139.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75809.1; -; Genomic_DNA.
DR EMBL; BT023445; AAY56436.1; -; mRNA.
DR EMBL; AK228139; BAF00096.1; -; mRNA.
DR RefSeq; NP_188262.1; NM_112512.5.
DR AlphaFoldDB; O04316; -.
DR SMR; O04316; -.
DR BioGRID; 6222; 2.
DR STRING; 3702.AT3G16410.1; -.
DR PaxDb; O04316; -.
DR PRIDE; O04316; -.
DR ProteomicsDB; 250658; -.
DR EnsemblPlants; AT3G16410.1; AT3G16410.1; AT3G16410.
DR GeneID; 820888; -.
DR Gramene; AT3G16410.1; AT3G16410.1; AT3G16410.
DR KEGG; ath:AT3G16410; -.
DR Araport; AT3G16410; -.
DR TAIR; locus:2088329; AT3G16410.
DR eggNOG; KOG0379; Eukaryota.
DR HOGENOM; CLU_030461_2_0_1; -.
DR InParanoid; O04316; -.
DR OMA; VNGMTSE; -.
DR OrthoDB; 266651at2759; -.
DR PhylomeDB; O04316; -.
DR PRO; PR:O04316; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O04316; baseline and differential.
DR Genevisible; O04316; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR GO; GO:0019762; P:glucosinolate catabolic process; IDA:TAIR.
DR GO; GO:0080028; P:nitrile biosynthetic process; IDA:TAIR.
DR CDD; cd09612; Jacalin; 2.
DR Gene3D; 2.100.10.30; -; 2.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR001229; Jacalin-like_lectin_dom.
DR InterPro; IPR033734; Jacalin-like_lectin_dom_plant.
DR InterPro; IPR036404; Jacalin-like_lectin_dom_sf.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR Pfam; PF01419; Jacalin; 2.
DR Pfam; PF01344; Kelch_1; 1.
DR SMART; SM00915; Jacalin; 2.
DR SMART; SM00612; Kelch; 3.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF51101; SSF51101; 2.
DR PROSITE; PS51752; JACALIN_LECTIN; 2.
PE 2: Evidence at transcript level;
KW Kelch repeat; Lectin; Reference proteome; Repeat.
FT CHAIN 1..619
FT /note="Nitrile-specifier protein 4"
FT /id="PRO_0000363145"
FT DOMAIN 2..142
FT /note="Jacalin-type lectin 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT DOMAIN 151..292
FT /note="Jacalin-type lectin 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01088"
FT REPEAT 196..242
FT /note="Kelch 1"
FT REPEAT 326..374
FT /note="Kelch 2"
FT REPEAT 379..425
FT /note="Kelch 3"
FT REPEAT 429..478
FT /note="Kelch 4"
FT REPEAT 480..524
FT /note="Kelch 5"
SQ SEQUENCE 619 AA; 67604 MW; 9FFB6666FBE71E73 CRC64;
MAQKVEAQGG NGGNQWDDGS EYDAVTKIQV AAGGNGIEYV KFTYVKNGQT EEAPLRGVKG
RSFEADPFVI NHPEEHLVSV EGRYNPEGLI LGLTFKSNKK TSDLIGYEDG TPFTLQVQDK
KIVGFYGFAG NNLHSLGAYF APLTNVTPLN AKKLEAKGGD TGDIWDDGVY DNVRKVYVGQ
AQYGIAFVKF EYVNGSQVVV GDEHGKKTEL GVEEFEIDAD DYIVYVEGYR EKVNGMTSEM
ITFLSFKTYK GKTSQPIEQR PGIKFVLQGG KIVGFHGRST DVLDSLGAYI SLSPTPNLHG
KWTKVDENGD GPGLRCSHDI AQVGNKIYSF GGEFTPNQPI DKHLYVFDIE SRTWSISPAT
GDIPTLSCLG VCMVSIGSTL YVFGGRDASR QYNGFYSFDT TTNEWKLLTP VEEGPTPRSF
HSMAADEENV YVFGGVSATA RLNTLDSYNI VDKKWFHCST PGDSLTARGG AGLEVVQGKV
WVVYGFNGCE VDDVHYYDPV QDKWTQVETF GVRPSERSVF ASAALGKHIV IFGGEIAMDP
LAHVGPGQLT DGTFALDTET LQWERLDKFG GEEETPSSRG WTASTTATIG GKKGLVMHGG
KAPTNDRFDD LFFYGIDSA
