JANA_DROME
ID JANA_DROME Reviewed; 135 AA.
AC P20348; A4V3M6; Q8T475; Q9VAB6;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Sex-regulated protein janus-A;
GN Name=janA; ORFNames=CG7933;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS A AND B),
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S;
RX PubMed=2503707; DOI=10.1128/mcb.9.6.2526-2535.1989;
RA Yanicostas C., Vincent A., Lepesant J.-A.;
RT "Transcriptional and posttranscriptional regulation contributes to the sex-
RT regulated expression of two sequence-related genes at the janus locus of
RT Drosophila melanogaster.";
RL Mol. Cell. Biol. 9:2526-2535(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: JanA and janB regulate somatic sex differentiation.
CC {ECO:0000269|PubMed:2503707}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B; Synonyms=jan2;
CC IsoId=P20348-1; Sequence=Displayed;
CC Name=A; Synonyms=C, jan1;
CC IsoId=P20348-2; Sequence=VSP_009384;
CC -!- TISSUE SPECIFICITY: Somatic and germline cells. Isoform B is expressed
CC in both sexes and in somatic and germ line cells. Isoform A is
CC expressed in males and is germ line specific.
CC {ECO:0000269|PubMed:2503707}.
CC -!- DEVELOPMENTAL STAGE: The non-sex-specific transcript is present at all
CC stages. {ECO:0000269|PubMed:2503707}.
CC -!- MISCELLANEOUS: Transcription of janA gives rise to two sex-specific and
CC one non-sex-specific transcripts.
CC -!- SIMILARITY: Belongs to the janus family. {ECO:0000305}.
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DR EMBL; M27033; AAC34203.1; -; Genomic_DNA.
DR EMBL; M27033; AAC34202.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN14209.1; -; Genomic_DNA.
DR EMBL; AE014297; AAO41613.1; -; Genomic_DNA.
DR EMBL; AE014297; AAS65231.1; -; Genomic_DNA.
DR EMBL; AY089320; AAL90058.1; -; mRNA.
DR PIR; A32317; A32317.
DR RefSeq; NP_788762.1; NM_176585.2. [P20348-1]
DR RefSeq; NP_788763.1; NM_176586.4. [P20348-2]
DR RefSeq; NP_996311.1; NM_206588.2. [P20348-2]
DR AlphaFoldDB; P20348; -.
DR SMR; P20348; -.
DR BioGRID; 68425; 7.
DR DIP; DIP-18771N; -.
DR STRING; 7227.FBpp0084962; -.
DR PaxDb; P20348; -.
DR PRIDE; P20348; -.
DR DNASU; 43569; -.
DR EnsemblMetazoa; FBtr0085595; FBpp0084961; FBgn0001280. [P20348-2]
DR EnsemblMetazoa; FBtr0085596; FBpp0084962; FBgn0001280. [P20348-1]
DR EnsemblMetazoa; FBtr0085597; FBpp0089165; FBgn0001280. [P20348-2]
DR GeneID; 43569; -.
DR KEGG; dme:Dmel_CG7933; -.
DR CTD; 43569; -.
DR FlyBase; FBgn0001280; janA.
DR VEuPathDB; VectorBase:FBgn0001280; -.
DR eggNOG; ENOG502S4DR; Eukaryota.
DR GeneTree; ENSGT00390000002738; -.
DR InParanoid; P20348; -.
DR PhylomeDB; P20348; -.
DR BioGRID-ORCS; 43569; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43569; -.
DR PRO; PR:P20348; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0001280; Expressed in wing disc and 62 other tissues.
DR ExpressionAtlas; P20348; baseline and differential.
DR Genevisible; P20348; DM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0101006; F:protein histidine phosphatase activity; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0035971; P:peptidyl-histidine dephosphorylation; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IEP:UniProtKB.
DR Gene3D; 3.50.20.20; -; 1.
DR InterPro; IPR007702; Janus.
DR InterPro; IPR038596; Janus_sf.
DR PANTHER; PTHR12258; PTHR12258; 1.
DR Pfam; PF05005; Ocnus; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Differentiation; Reference proteome;
KW Sexual differentiation.
FT CHAIN 1..135
FT /note="Sex-regulated protein janus-A"
FT /id="PRO_0000206158"
FT ACT_SITE 63
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 37
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 104..106
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..16
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_009384"
FT CONFLICT 44
FT /note="F -> I (in Ref. 4; AAL90058)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="A -> T (in Ref. 4; AAL90058)"
FT /evidence="ECO:0000305"
FT CONFLICT 94
FT /note="E -> D (in Ref. 4; AAL90058)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 135 AA; 15220 MW; 2720237CE77F3132 CRC64;
MNRLQLLSKG LRLIHKMSEE ALAGVPLVHI SPEGIFKYVM INVFDGGDAS KAVIRGFADC
TWHADIFERE EEVFKKLGLR AECPGGGRIE HNPEKKYLKV YGYSQGFGKA DHAQTKRILA
TKYPDYTIEI SDEGY
