ID   JANB_PENJA              Reviewed;         243 AA.
AC   A0A0E3D8Q2;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   24-JUN-2015, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Terpene cyclase janB {ECO:0000303|PubMed:26213965};
DE            EC=4.2.3.- {ECO:0000305|PubMed:26213965};
DE   AltName: Full=Janthitremanes biosynthesis cluster protein B {ECO:0000303|PubMed:26213965};
GN   Name=janB {ECO:0000303|PubMed:26213965};
OS   Penicillium janthinellum (Penicillium vitale).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=5079;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, FUNCTION, AND PATHWAY.
RC   STRAIN=PN2408;
RX   PubMed=26213965; DOI=10.3390/toxins7082701;
RA   Nicholson M.J., Eaton C.J., Starkel C., Tapper B.A., Cox M.P., Scott B.;
RT   "Molecular cloning and functional analysis of gene clusters for the
RT   biosynthesis of indole-diterpenes in Penicillium crustosum and P.
RT   janthinellum.";
RL   Toxins 7:2701-2722(2015).
CC   -!- FUNCTION: Terpene cyclase; part of the gene cluster that mediates the
CC       biosynthesis of the indole diterpenes janthitremanes such as shearinine
CC       K or shearinine A (PubMed:26213965). The geranylgeranyl diphosphate
CC       (GGPP) synthase janG catalyzes the first step in janthitremane
CC       biosynthesis via conversion of farnesyl pyrophosphate and isopentyl
CC       pyrophosphate into geranylgeranyl pyrophosphate (GGPP)
CC       (PubMed:26213965). Condensation of indole-3-glycerol phosphate with
CC       GGPP by the prenyl transferase janC then forms 3-geranylgeranylindole
CC       (3-GGI) (PubMed:26213965). Epoxidation by the FAD-dependent
CC       monooxygenase janM leads to a epoxidized-GGI that is substrate of the
CC       terpene cyclase janB for cyclization to yield paspaline
CC       (PubMed:26213965). Paspaline is subsequently converted to 13-
CC       desoxypaspaline by the cytochrome P450 monooxygenase janP, via beta-PC-
CC       M6 in a series of alpha-face oxidations (Probable). The cytochrome P450
CC       monooxygenase janQ is proposed to carry out sequential beta-face
CC       oxidation steps at C-7 and C-13 of 13-desoxypaspaline to form
CC       paspalicine and paspalinine respectively (Probable). The indole
CC       diterpene prenyltransferase janD may then convert paspalinine into
CC       shearinine K which is substrate of janO and/or additional enzymes for
CC       oxidation and cyclization to generate shearinine A (Probable).
CC       {ECO:0000269|PubMed:26213965, ECO:0000305|PubMed:26213965}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:26213965}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the paxB family. {ECO:0000305}.
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DR   EMBL; KF280651; AGZ20474.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0E3D8Q2; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR039020; PaxB-like.
DR   PANTHER; PTHR42038; PTHR42038; 1.
PE   3: Inferred from homology;
KW   Lyase; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..243
FT                   /note="Terpene cyclase janB"
FT                   /id="PRO_0000446544"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        112..132
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   243 AA;  27112 MW;  892490FF23E87F71 CRC64;
     MDGFDVSQAP REYQAVKPLA DLFVLGMGLG WVINYVGMVY TSFKERTYGM AIMPLCCNIA
     WEIVYCVFHP SKSRVELGVF AMGLLINFGV MYAAIIFSSR EWSHAPLVER NLPWIFCIGV
     LGFLTGHLAL AAEIGPSLAY SWGAVVCQLL LSVGGLCQLL CRGSTRGASY TLWLSRFLGS
     CCTVGFASLR WMYWPQSFAW LNSPLVLWSL AVFLMVDGSY GVCFWYVEQY EKSVLMGRAT
     KAM