ID   JAP2_RANJA              Reviewed;          21 AA.
AC   P83306;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Japonicin-2;
OS   Rana japonica (Japanese reddish frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Rana; Rana.
OX   NCBI_TaxID=8402 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion;
RX   PubMed=11835990; DOI=10.1016/s0196-9781(01)00634-9;
RA   Isaacson T., Soto A., Iwamuro S., Knoop F.C., Conlon J.M.;
RT   "Antimicrobial peptides with atypical structural features from the skin of
RT   the Japanese brown frog Rana japonica.";
RL   Peptides 23:419-425(2002).
CC   -!- FUNCTION: Antibacterial activity against the Gram-negative bacterium
CC       E.coli and the Gram-positive bacterium S.aureus.
CC       {ECO:0000269|PubMed:11835990}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- MASS SPECTROMETRY: Mass=2685.4; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:11835990};
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DR   AlphaFoldDB; P83306; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016999; P:antibiotic metabolic process; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PEPTIDE         1..21
FT                   /note="Japonicin-2"
FT                   /id="PRO_0000043807"
FT   DISULFID        14..21
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   21 AA;  2358 MW;  AB055DF897566BDF CRC64;
     FGLPMLSILP KALCILLKRK C