ID   JAR6_NICAT              Reviewed;         580 AA.
AC   A0A314KSQ4; B0VXR3;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   13-FEB-2019, sequence version 2.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Jasmonoyl--L-amino acid synthetase JAR6 {ECO:0000305};
DE            EC=6.3.2.52 {ECO:0000269|PubMed:17273867};
DE   AltName: Full=Jasmonate-amino acid synthetase JAR6 {ECO:0000305};
DE   AltName: Full=Jasmonic acid-amido synthetase JAR6 {ECO:0000305};
DE   AltName: Full=Protein JASMONATE RESISTANT 6 {ECO:0000305};
GN   Name=JAR6; ORFNames=A4A49_19048 {ECO:0000312|EMBL:OIT32368.1};
OS   Nicotiana attenuata (Coyote tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=49451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND INDUCTION.
RX   PubMed=17273867; DOI=10.1007/s00425-007-0477-3;
RA   Wang L., Halitschke R., Kang J.H., Berg A., Harnisch F., Baldwin I.T.;
RT   "Independently silencing two JAR family members impairs levels of trypsin
RT   proteinase inhibitors but not nicotine.";
RL   Planta 226:159-167(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=28536194; DOI=10.1073/pnas.1700073114;
RA   Xu S., Brockmoeller T., Navarro-Quezada A., Kuhl H., Gase K., Ling Z.,
RA   Zhou W., Kreitzer C., Stanke M., Tang H., Lyons E., Pandey P., Pandey S.P.,
RA   Timmermann B., Gaquerel E., Baldwin I.T.;
RT   "Wild tobacco genomes reveal the evolution of nicotine biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 114:6133-6138(2017).
CC   -!- FUNCTION: Catalyzes the synthesis of jasmonate-amino acid conjugates by
CC       adenylation (PubMed:17273867). Catalyzes the conjugation of jasmonate
CC       (JA) to Ile, Leu and Val (PubMed:17273867). Catalyzes the conjugation
CC       of JA to Ile that may mediate defense signaling and resistance to the
CC       herbivore Manduca sexta caterpillars (PubMed:17273867).
CC       {ECO:0000269|PubMed:17273867}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a jasmonate + an L-alpha-amino acid + ATP = a jasmonyl-L-amino
CC         acid + AMP + diphosphate + H(+); Xref=Rhea:RHEA:55772,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:59869, ChEBI:CHEBI:136183, ChEBI:CHEBI:136184,
CC         ChEBI:CHEBI:456215; EC=6.3.2.52;
CC         Evidence={ECO:0000269|PubMed:17273867};
CC   -!- INDUCTION: Induced by wounding and oral secretion of the herbivore
CC       Manduca sexta caterpillars. {ECO:0000269|PubMed:17273867}.
CC   -!- SIMILARITY: Belongs to the IAA-amido conjugating enzyme family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=OIT32368.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; DQ359730; ABC87761.1; -; mRNA.
DR   EMBL; MJEQ01001075; OIT32368.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_019225904.1; XM_019370359.1.
DR   RefSeq; XP_019225905.1; XM_019370360.1.
DR   AlphaFoldDB; A0A314KSQ4; -.
DR   SMR; A0A314KSQ4; -.
DR   STRING; 49451.A0A314KSQ4; -.
DR   EnsemblPlants; OIT32368; OIT32368; A4A49_19048.
DR   GeneID; 109207440; -.
DR   Gramene; OIT32368; OIT32368; A4A49_19048.
DR   KEGG; nau:109207440; -.
DR   Proteomes; UP000187609; Unassembled WGS sequence.
DR   GO; GO:0102053; F:(-)-jasmonoyl-isoleucine synthetase activity; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0102058; F:jasmonoyl-leucine synthetase activity; IDA:UniProtKB.
DR   GO; GO:0102057; F:jasmonoyl-valine synthetase activity; IDA:UniProtKB.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR004993; GH3.
DR   PANTHER; PTHR31901; PTHR31901; 1.
DR   Pfam; PF03321; GH3; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Jasmonic acid signaling pathway; Ligase; Nucleotide-binding;
KW   Plant defense; Reference proteome.
FT   CHAIN           1..580
FT                   /note="Jasmonoyl--L-amino acid synthetase JAR6"
FT                   /id="PRO_0000446307"
FT   BINDING         100
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         103
FT                   /ligand="jasmonate"
FT                   /ligand_id="ChEBI:CHEBI:58431"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         120
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         123
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         164
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         167..171
FT                   /ligand="an L-alpha-amino acid"
FT                   /ligand_id="ChEBI:CHEBI:59869"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         169
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         329..332
FT                   /ligand="jasmonate"
FT                   /ligand_id="ChEBI:CHEBI:58431"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         332..337
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   BINDING         534..538
FT                   /ligand="an L-alpha-amino acid"
FT                   /ligand_id="ChEBI:CHEBI:59869"
FT                   /evidence="ECO:0000250|UniProtKB:Q9SKE2"
FT   CONFLICT        247
FT                   /note="R -> K (in Ref. 1; ABC87761)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        254
FT                   /note="R -> G (in Ref. 1; ABC87761)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        532
FT                   /note="F -> S (in Ref. 1; ABC87761)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   580 AA;  64781 MW;  F9B9A36EE97D7FC3 CRC64;
     MKMLVEKIEK KFDQEKVIEE FEDLTKDAGK IQEETLKKIL EQNGGTEYLQ LWGLNGRTDP
     QTFKNCVPIV THNDLEPYIQ RIADGDLSPI LTGKPIETIS LSSGTTQGKP KFVPFNDELM
     ESTMQIFKTS FAFRNREFPI GNGKALQFIY SSKQFKTKGG LAAGTATTNV YRNAQFKKTM
     KAMCTPCCSP DEVIFGPDFH QSLYCHLLCG LIFHDEVQVV SSTFAHSIVH AFRTFEQVWE
     ALVVDIREGV LSSRVTVPSI RLAMSKLLKP DPELADTIYN KCSRLSNWYG LIPDLFPNTR
     YIYGIMTGSM EPYLKKLRHY AGELPLLSAD YGSSEGWVGV NVNPKLPPEL VTYAVLPNIG
     YFEFIPLGGN LNGIEQANSP VGLTEVKLGE EYEVVFTNFA GLYRYRLGDV VKVKGFHNGT
     PELQFVCRSN LLLSINIDKN TEKDLQLAVE AAAKRLVDEK LEVVDFTSHV NVSADPGHYV
     IFWELSGEAT DEMLQDCCNC LDRSFIDAGY VSSRKVNAIG ALELRIVKRG TFHKILDHFV
     GLGGAVSQFK TPRCVGPKNS SLLQILSSNV VETYVSTAFC