JCAD_HUMAN
ID JCAD_HUMAN Reviewed; 1359 AA.
AC Q9P266; Q5HYA7; Q5T992; Q86WZ9; Q9BYJ2;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 3.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Junctional cadherin 5-associated protein {ECO:0000305};
DE AltName: Full=Junctional protein associated with coronary artery disease;
DE Short=JCAD;
GN Name=JCAD {ECO:0000312|HGNC:HGNC:29283};
GN Synonyms=KIAA1462 {ECO:0000303|PubMed:10819331};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLY-957.
RC TISSUE=Brain;
RX PubMed=10819331; DOI=10.1093/dnares/7.2.143;
RA Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XVII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:143-150(2000).
RN [2]
RP SEQUENCE REVISION.
RX PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT "Construction of expression-ready cDNA clones for KIAA genes: manual
RT curation of 330 KIAA cDNA clones.";
RL DNA Res. 9:99-106(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 272-1359, AND VARIANTS GLY-957
RP AND THR-1002.
RC TISSUE=Lymph node, and Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 851-1359, AND VARIANT GLY-957.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1044 AND SER-1194, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- SUBCELLULAR LOCATION: Cell junction, adherens junction
CC {ECO:0000250|UniProtKB:Q5DTX6}. Note=Colocalizes with CDH5/VE-Cadherin
CC in endothelial cells but not in epithelial cells.
CC {ECO:0000250|UniProtKB:Q5DTX6}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA95986.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAI46027.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB040895; BAA95986.2; ALT_INIT; mRNA.
DR EMBL; AL158036; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471072; EAW86016.1; -; Genomic_DNA.
DR EMBL; AL583916; CAC29500.1; -; mRNA.
DR EMBL; BX648773; CAI46027.1; ALT_INIT; mRNA.
DR EMBL; BC047548; AAH47548.1; -; mRNA.
DR CCDS; CCDS41500.1; -.
DR RefSeq; NP_065899.1; NM_020848.2.
DR RefSeq; XP_011517910.1; XM_011519608.1.
DR AlphaFoldDB; Q9P266; -.
DR BioGRID; 121655; 25.
DR IntAct; Q9P266; 11.
DR MINT; Q9P266; -.
DR STRING; 9606.ENSP00000364526; -.
DR GlyGen; Q9P266; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9P266; -.
DR PhosphoSitePlus; Q9P266; -.
DR BioMuta; JCAD; -.
DR DMDM; 166218835; -.
DR EPD; Q9P266; -.
DR jPOST; Q9P266; -.
DR MassIVE; Q9P266; -.
DR MaxQB; Q9P266; -.
DR PaxDb; Q9P266; -.
DR PeptideAtlas; Q9P266; -.
DR PRIDE; Q9P266; -.
DR ProteomicsDB; 83737; -.
DR Antibodypedia; 2803; 25 antibodies from 11 providers.
DR DNASU; 57608; -.
DR Ensembl; ENST00000375377.2; ENSP00000364526.1; ENSG00000165757.9.
DR GeneID; 57608; -.
DR KEGG; hsa:57608; -.
DR MANE-Select; ENST00000375377.2; ENSP00000364526.1; NM_020848.4; NP_065899.1.
DR UCSC; uc001iux.4; human.
DR CTD; 57608; -.
DR DisGeNET; 57608; -.
DR GeneCards; JCAD; -.
DR HGNC; HGNC:29283; JCAD.
DR HPA; ENSG00000165757; Low tissue specificity.
DR MIM; 614398; gene.
DR neXtProt; NX_Q9P266; -.
DR OpenTargets; ENSG00000165757; -.
DR PharmGKB; PA134955526; -.
DR VEuPathDB; HostDB:ENSG00000165757; -.
DR eggNOG; ENOG502QURJ; Eukaryota.
DR GeneTree; ENSGT00390000015348; -.
DR HOGENOM; CLU_005347_0_0_1; -.
DR InParanoid; Q9P266; -.
DR OMA; STFHTSE; -.
DR OrthoDB; 208373at2759; -.
DR PhylomeDB; Q9P266; -.
DR TreeFam; TF335913; -.
DR PathwayCommons; Q9P266; -.
DR SignaLink; Q9P266; -.
DR BioGRID-ORCS; 57608; 11 hits in 1069 CRISPR screens.
DR ChiTaRS; KIAA1462; human.
DR GenomeRNAi; 57608; -.
DR Pharos; Q9P266; Tbio.
DR PRO; PR:Q9P266; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q9P266; protein.
DR Bgee; ENSG00000165757; Expressed in saphenous vein and 192 other tissues.
DR Genevisible; Q9P266; HS.
DR GO; GO:0005912; C:adherens junction; IDA:BHF-UCL.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
DR GO; GO:0032587; C:ruffle membrane; IDA:BHF-UCL.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:1903589; P:positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis; IMP:BHF-UCL.
DR GO; GO:0090050; P:positive regulation of cell migration involved in sprouting angiogenesis; IMP:BHF-UCL.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; IMP:BHF-UCL.
DR GO; GO:1903672; P:positive regulation of sprouting angiogenesis; IEA:Ensembl.
DR GO; GO:1900748; P:positive regulation of vascular endothelial growth factor signaling pathway; IMP:BHF-UCL.
DR InterPro; IPR028221; JCAD.
DR PANTHER; PTHR34757; PTHR34757; 1.
DR Pfam; PF15351; JCAD; 1.
PE 1: Evidence at protein level;
KW Cell adhesion; Cell junction; Phosphoprotein; Reference proteome.
FT CHAIN 1..1359
FT /note="Junctional cadherin 5-associated protein"
FT /id="PRO_0000314185"
FT REGION 1..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 454..554
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..813
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 840..1076
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1105..1141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1157..1207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1225..1260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1276..1359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..77
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..137
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..351
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..382
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 535..554
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 605..645
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 649..683
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 684..703
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 725..739
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 750..777
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 848..863
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 864..884
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1322..1359
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1044
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 1050
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5DTX6"
FT MOD_RES 1194
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 1281
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5DTX6"
FT VARIANT 487
FT /note="E -> D (in dbSNP:rs7917573)"
FT /id="VAR_037867"
FT VARIANT 494
FT /note="D -> N (in dbSNP:rs7917566)"
FT /id="VAR_037868"
FT VARIANT 729
FT /note="E -> A (in dbSNP:rs7901855)"
FT /id="VAR_037869"
FT VARIANT 957
FT /note="R -> G (in dbSNP:rs2185724)"
FT /evidence="ECO:0000269|PubMed:10819331,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:17974005"
FT /id="VAR_037870"
FT VARIANT 1002
FT /note="S -> T (in dbSNP:rs3739998)"
FT /evidence="ECO:0000269|PubMed:17974005"
FT /id="VAR_037871"
FT VARIANT 1095
FT /note="A -> G (in dbSNP:rs12240677)"
FT /id="VAR_037872"
FT CONFLICT 813
FT /note="K -> R (in Ref. 5; CAI46027)"
FT /evidence="ECO:0000305"
FT CONFLICT 852
FT /note="S -> G (in Ref. 6; AAH47548)"
FT /evidence="ECO:0000305"
FT CONFLICT 1083
FT /note="Q -> R (in Ref. 6; AAH47548)"
FT /evidence="ECO:0000305"
FT CONFLICT 1348
FT /note="P -> R (in Ref. 6; AAH47548)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1359 AA; 148351 MW; 0104E6B4A7946D1F CRC64;
MYSVEDLLIS HGYKLSRDPP ASREDNPKGR QAARTGTRAG QGLQNGHEDG PAALAHRKTS
AGKGHVSDSE SRRSTPRGHG EPQSTSASRT SEAGFCNQPP SAWSSHPPTG NDQAYRRRGR
QEARSQKPRE HENLEARGMA QAHSLPVHVR EGPWEVGGRS EHVMKKPVWE EELRMSGPAK
WQNVSLESWN QPRKLGRQMS DGDGERLFQD LYPFIQGEHV LNSQNKGKSR SLPRVLSPES
LSCTEIPIPL NERHSPKMPP YPPTCAPNLD STRNSEKSGC SAPFPRPKFG RPLKPPSYSS
HQQSRGGADS SDSQDSQQMD AYVPRHELCL SDPGLEPPVY VPPPSYRSPP QNIPNPYLED
TVPINVCGGH SQQQSPTEKA GASGQPPSGP PGTGNEYGVS PRLPQGLPAH PRPVTAYDGF
VQYIPFDDPR LRHFKLAQPQ GFCEDIKLDD KSYNSSPVTA QEPAHGGMQP DGAIWNPQSL
IPPSGDERGL VLADSSPRWL WGQPPGDGEN SGLPNQRDRC VARGQWPDVR GSQHGHTGRQ
VSSPYSQGES TCETQTKLKK FQTGTRTKKS SKKKMNETIF CLVSIPVKSE SHLPDRDMDN
NDLKPSADQK NGSDKSPALQ EQSLLSMSST DLELQALTGS MGGRTEFQKQ DLGEPEEDRQ
TNDLSFIHLT KHRELKHSGS WPGHRYRDQQ TQTSFSEEPQ SSQLLPGAKL GGPSRAALSP
KCSDPAASEA QTHTAFPTGD HKQRPSARNL KGHRSLSPSS NSAFSRTSLS VDQAPTPKAG
RSQPCVDVHG LGAHPGPKRE VVKGEPTGPC NSKQLFGQFL LKPVSRRPWD LISQLESFNK
ELQEEEESSS SSSSSSSSSE ESEAEPQQEN RAHCRQEDVG FRGNSPEMRV EPQPRMWVPE
SPVCRSGRGE SKSESWSEEL QPGHPRAWPP SPGRFRVEEG GGAPFCSADG STSAEKRHLE
VSNGMDELAG SPFPVTRMSS RSSDAKPLPA SYPAEPREPQ ESPKITSAFS SVKPSEAVPR
KFDSGGERGA GLPLSLSNKN RGLSAPDLRS VGLTPGQEQG ASELEGSLGE ASTIEIPPGE
SLQARAARIL GIEVAVESLL PGIRRAGQNQ PAEPDASACT PESPQEELLS RPAPADVPRV
STDAFYGRRK CGWTKSPLFV GDRDSARRAP QAFEHSDVDG VVTSTDPVPE PEPSPLESKF
FEQKDVETKP PFRSTLFHFV ERTPSVAGSE KRLRSPSKVI ESLQEKLASP PRRADPDRLM
RMKEVSSVSR MRVLSFRNAD SQEDAEELKA TTRGQAGLPG GLVSPGSGDR AQRLGHSLSV
SKDSISREEK EHPAAQKEKS MDQDFWCPDS YDPSRVERV
