JCAD_MOUSE
ID JCAD_MOUSE Reviewed; 1320 AA.
AC Q5DTX6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Junctional cadherin 5-associated protein {ECO:0000305};
DE AltName: Full=Junctional protein associated with coronary artery disease;
DE Short=JCAD {ECO:0000303|PubMed:21884682};
GN Name=Jcad {ECO:0000303|PubMed:21884682}; Synonyms=Gm328, Kiaa1462;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 563-1320.
RC TISSUE=Fetal brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-484 AND SER-486, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-484; SER-486; SER-851;
RP SER-1033; SER-1245 AND SER-1248, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=21884682; DOI=10.1016/j.bbrc.2011.08.073;
RA Akashi M., Higashi T., Masuda S., Komori T., Furuse M.;
RT "A coronary artery disease-associated gene product, JCAD/KIAA1462, is a
RT novel component of endothelial cell-cell junctions.";
RL Biochem. Biophys. Res. Commun. 413:224-229(2011).
CC -!- SUBCELLULAR LOCATION: Cell junction, adherens junction
CC {ECO:0000269|PubMed:21884682}. Note=Colocalizes with CDH5/VE-Cadherin
CC in endothelial cells but not in epithelial cells.
CC {ECO:0000269|PubMed:21884682}.
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DR EMBL; AC147227; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK220394; BAD90447.1; -; mRNA.
DR CCDS; CCDS37719.1; -.
DR RefSeq; NP_001075432.1; NM_001081963.1.
DR RefSeq; XP_006525966.1; XM_006525903.2.
DR RefSeq; XP_006525967.1; XM_006525904.3.
DR AlphaFoldDB; Q5DTX6; -.
DR SMR; Q5DTX6; -.
DR BioGRID; 232179; 4.
DR STRING; 10090.ENSMUSP00000038613; -.
DR iPTMnet; Q5DTX6; -.
DR PhosphoSitePlus; Q5DTX6; -.
DR EPD; Q5DTX6; -.
DR jPOST; Q5DTX6; -.
DR MaxQB; Q5DTX6; -.
DR PaxDb; Q5DTX6; -.
DR PRIDE; Q5DTX6; -.
DR ProteomicsDB; 268910; -.
DR Antibodypedia; 2803; 25 antibodies from 11 providers.
DR Ensembl; ENSMUST00000037029; ENSMUSP00000038613; ENSMUSG00000033960.
DR Ensembl; ENSMUST00000234153; ENSMUSP00000157313; ENSMUSG00000033960.
DR GeneID; 240185; -.
DR KEGG; mmu:240185; -.
DR UCSC; uc008dyl.1; mouse.
DR CTD; 57608; -.
DR MGI; MGI:2685174; Jcad.
DR VEuPathDB; HostDB:ENSMUSG00000033960; -.
DR eggNOG; ENOG502QURJ; Eukaryota.
DR GeneTree; ENSGT00390000015348; -.
DR HOGENOM; CLU_005347_0_0_1; -.
DR InParanoid; Q5DTX6; -.
DR OMA; STFHTSE; -.
DR OrthoDB; 208373at2759; -.
DR PhylomeDB; Q5DTX6; -.
DR TreeFam; TF335913; -.
DR BioGRID-ORCS; 240185; 4 hits in 71 CRISPR screens.
DR PRO; PR:Q5DTX6; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q5DTX6; protein.
DR Bgee; ENSMUSG00000033960; Expressed in gastrula and 231 other tissues.
DR ExpressionAtlas; Q5DTX6; baseline and differential.
DR Genevisible; Q5DTX6; MM.
DR GO; GO:0005912; C:adherens junction; ISO:MGI.
DR GO; GO:0030054; C:cell junction; IDA:MGI.
DR GO; GO:0005911; C:cell-cell junction; IDA:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0032587; C:ruffle membrane; ISO:MGI.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:1903589; P:positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis; ISO:MGI.
DR GO; GO:0090050; P:positive regulation of cell migration involved in sprouting angiogenesis; ISO:MGI.
DR GO; GO:0043410; P:positive regulation of MAPK cascade; ISO:MGI.
DR GO; GO:1903672; P:positive regulation of sprouting angiogenesis; IMP:BHF-UCL.
DR GO; GO:1900748; P:positive regulation of vascular endothelial growth factor signaling pathway; ISO:MGI.
DR InterPro; IPR028221; JCAD.
DR PANTHER; PTHR34757; PTHR34757; 1.
DR Pfam; PF15351; JCAD; 1.
PE 1: Evidence at protein level;
KW Cell adhesion; Cell junction; Phosphoprotein; Reference proteome.
FT CHAIN 1..1320
FT /note="Junctional cadherin 5-associated protein"
FT /id="PRO_0000314186"
FT REGION 1..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 183..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 252..426
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 452..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 676..720
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 741..802
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 835..942
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1085..1116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1153..1174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1275..1320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..33
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..87
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 306..332
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 339..360
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 367..383
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 681..720
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 741..783
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 835..850
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 865..881
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 890..904
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1085..1112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1293..1320
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 484
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 486
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 851
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1027
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P266"
FT MOD_RES 1033
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1245
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1248
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 1320 AA; 144798 MW; 2317F13096AFE1E7 CRC64;
MYSVEDLLIS HGYKPARDAA APCEDKSERC RSTRTGPRAG QGLLNGYKDG ATAHTHSRTS
LGTGHVSNSE NRISRPRGHR EHQSTSRTPE ARFLNQPSLA WSSQPQSGRD DIYWSRGRQE
GSGSLCPRDW KELESRGMAQ AYSLPVHVRE NLWEVAGRTE HVMKNAIWEE ELRMQDMSLE
SWKKPRELGR QASDGDGRKR PQEKFEGLYP FVHGEHTSQN RKKSQSLPRA LSPKSLNFTE
IPVPLHDGHI TGVPKVPPYP PSFPSPSEPM RNLEKASSSG PFPRPKFGKP LKTPCYSSHS
QPRGEGGFQD HQHRDPRGSY PTRSKDPSHE LGMLDPGLEP PVYVPPPSYR SPPQHIPNPY
LEDPVPRHVS SNQSQQQVPE KPETSCPLPS GSLAARDLYD AMPGSPPQGL PPQPYPIATH
GGSIQYIPFD DPRIRHIKLA QPPEFYEEAK LDDTSYNPGL LTTQEPAIGK RQYDDAPSVP
RGPTPSPVNE QSSAFVHSSP RWLQGQLPLG IGPGGFHGQT EHHVMGGLTT NVTDIKAEGH
ASSPQPQSEG TCKTYTKLRK FETGVQSKKS SKKKSNATIF CLVSIPVKSE SLVLATDTNN
NDFKLVADKT RGLCQGSALQ EQSLLSMSST DLELQALMGS MAWRRTSPRQ GLRESEDGQI
DDPRILHLIK PKELQASSPW PGHQYRDQQT QTSFHEDSKS SQLLPATKPG EASNVAPTPT
CPDTTASEVC LHTALAFSDQ NQKPSVPHLQ GQTSLSPSRN SAFSRTSSAI NQASMSKGTS
DQLPGANPVP KPEVVKGEST TGQCNSTQLF GQFLLKPVSR RPWDLISQLE SFNKELQEEE
ESHGGSGSED SEAEQPEDCA DSRTKSWALQ GTRTAQQPAG LALENVASPD RRLNDSQSWN
EEPKPGHSSV HPQSLGPSQE EGSRGVPVQW ADGSLTAEQK SQEDLNGMCE RDFSPRPVSR
IAPIDTKAAP LYCLSEPRGS QELTKFGDAV GSVQLGRETP TQVGNGGDTE VLPCVLLPLA
DKYRGHSTPD FRSLELTLGQ EQNAYKLECL DLENTVEVLP SESLQERAER ILGIEVAVES
LLPSARRTEQ SQLPEPDASA CNPSSSREDS SHSLALPVGP KVATDAFYGR RKCGWTESPL
FVGERAPQAS ICSDVDGFPT SQATSPEPGK KDEEAKAPFK STLFHFMEKS TNVVGPEKRL
RNPSKVVENL QEKLVSPPKK ADSVHLIRMR EVNSLSQMRC LSSKSADSVE EPDPLKVIKS
SAWLSEGLTS LGGKDEAWQA GHLPSVSQNE NGHPEVPRDK MSDQDLWCAD SYDPSRVERV
