ID   JEFA_MYCTO              Reviewed;         508 AA.
AC   P9WJW8; L0TCL0; O53186; Q7D739;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Drug efflux pump JefA {ECO:0000250|UniProtKB:P9WJW9};
GN   Name=jefA {ECO:0000250|UniProtKB:P9WJW9}; OrderedLocusNames=MT2534;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Involved in resistance to ethambutol and isoniazid.
CC       {ECO:0000250|UniProtKB:P9WJW9}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK46834.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK46834.1; ALT_INIT; Genomic_DNA.
DR   PIR; B70865; B70865.
DR   RefSeq; WP_003412642.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WJW8; -.
DR   SMR; P9WJW8; -.
DR   EnsemblBacteria; AAK46834; AAK46834; MT2534.
DR   GeneID; 45426449; -.
DR   KEGG; mtc:MT2534; -.
DR   PATRIC; fig|83331.31.peg.2735; -.
DR   HOGENOM; CLU_000960_28_2_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..508
FT                   /note="Drug efflux pump JefA"
FT                   /id="PRO_0000427757"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        265..285
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        328..348
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        354..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        399..419
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        479..499
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   508 AA;  53479 MW;  382D710B286F71C3 CRC64;
     MTPRQRLTVL ATGLGIFMVF VDVNIVNVAL PSIQKVFHTG EQGLQWAVAG YSLGMAAVLM
     SCALLGDRYG RRRSFVFGVT LFVVSSIVCV LPVSLAVFTV ARVIQGLGAA FISVLSLALL
     SHSFPNPRMK ARAISNWMAI GMVGAASAPA LGGLMVDGLG WRSVFLVNVP LGAIVWLLTL
     VGVDESQDPE PTQLDWVGQL TLIPAVALIA YTIIEAPRFD RQSAGFVAAL LLAAGVLLWL
     FVRHEHRAAF PLVDLKLFAE PLYRSVLIVY FVVMSCFFGT LMVITQHFQN VRDLSPLHAG
     LMMLPVPAGF GVASLLAGRA VNKWGPQLPV LTCLAAMFIG LAIFAISMDH AHPVALVGLT
     IFGAGAGGCA TPLLHLGMTK VDDGRAGMAA GMLNLQRSLG GIFGVAFLGT IVAAWLGAAL
     PNTMADEIPD PIARAIVVDV IVDSANPHAH AAFIGPGHRI TAAQEDEIVL AADAVFVSGI
     KLALGGAAVL LTGAFVLGWT RFPRTPAS