JEFA_MYCTU
ID JEFA_MYCTU Reviewed; 508 AA.
AC P9WJW9; L0TCL0; O53186; Q7D739;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Drug efflux pump JefA {ECO:0000305};
GN Name=jefA {ECO:0000303|PubMed:20716818};
GN OrderedLocusNames=Rv2459 {ECO:0000312|EMBL:CCP45252.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION, ACTIVITY REGULATION, AND INDUCTION.
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX PubMed=20716818;
RA Gupta A.K., Reddy V.P., Lavania M., Chauhan D.S., Venkatesan K.,
RA Sharma V.D., Tyagi A.K., Katoch V.M.;
RT "jefA (Rv2459), a drug efflux gene in Mycobacterium tuberculosis confers
RT resistance to isoniazid & ethambutol.";
RL Indian J. Med. Res. 132:176-188(2010).
CC -!- FUNCTION: Involved in resistance to ethambutol and isoniazid.
CC {ECO:0000269|PubMed:20716818}.
CC -!- ACTIVITY REGULATION: Efflux activity is inhibited by carbonyl cyanide
CC m-chlorophenylhydrazone (CCCP) and verapamil.
CC {ECO:0000269|PubMed:20716818}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- INDUCTION: Induced in the presence of ethambutol or isoniazid.
CC {ECO:0000269|PubMed:20716818}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP45252.1; -; Genomic_DNA.
DR PIR; B70865; B70865.
DR RefSeq; NP_216975.1; NC_000962.3.
DR RefSeq; WP_003412642.1; NZ_NVQJ01000024.1.
DR AlphaFoldDB; P9WJW9; -.
DR SMR; P9WJW9; -.
DR STRING; 83332.Rv2459; -.
DR PaxDb; P9WJW9; -.
DR GeneID; 45426449; -.
DR GeneID; 888191; -.
DR KEGG; mtu:Rv2459; -.
DR TubercuList; Rv2459; -.
DR eggNOG; COG0477; Bacteria.
DR OMA; FAVCISK; -.
DR PhylomeDB; P9WJW9; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..508
FT /note="Drug efflux pump JefA"
FT /id="PRO_0000390685"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 508 AA; 53479 MW; 382D710B286F71C3 CRC64;
MTPRQRLTVL ATGLGIFMVF VDVNIVNVAL PSIQKVFHTG EQGLQWAVAG YSLGMAAVLM
SCALLGDRYG RRRSFVFGVT LFVVSSIVCV LPVSLAVFTV ARVIQGLGAA FISVLSLALL
SHSFPNPRMK ARAISNWMAI GMVGAASAPA LGGLMVDGLG WRSVFLVNVP LGAIVWLLTL
VGVDESQDPE PTQLDWVGQL TLIPAVALIA YTIIEAPRFD RQSAGFVAAL LLAAGVLLWL
FVRHEHRAAF PLVDLKLFAE PLYRSVLIVY FVVMSCFFGT LMVITQHFQN VRDLSPLHAG
LMMLPVPAGF GVASLLAGRA VNKWGPQLPV LTCLAAMFIG LAIFAISMDH AHPVALVGLT
IFGAGAGGCA TPLLHLGMTK VDDGRAGMAA GMLNLQRSLG GIFGVAFLGT IVAAWLGAAL
PNTMADEIPD PIARAIVVDV IVDSANPHAH AAFIGPGHRI TAAQEDEIVL AADAVFVSGI
KLALGGAAVL LTGAFVLGWT RFPRTPAS
