JGB_ARATH
ID JGB_ARATH Reviewed; 465 AA.
AC O48716;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Protein JINGUBANG {ECO:0000303|PubMed:27468890};
GN Name=JGB {ECO:0000303|PubMed:27468890};
GN OrderedLocusNames=At2g26490 {ECO:0000312|Araport:AT2G26490};
GN ORFNames=T9J22.16 {ECO:0000312|EMBL:AAC14498.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-344, INTERACTION WITH TCP4, AND
RP 3D-STRUCTURE MODELING.
RX PubMed=27468890; DOI=10.1105/tpc.16.00401;
RA Ju Y., Guo L., Cai Q., Ma F., Zhu Q.Y., Zhang Q., Sodmergen S.;
RT "Arabidopsis JINGUBANG is a negative regulator of pollen germination that
RT prevents pollination in moist environments.";
RL Plant Cell 28:2131-2146(2016).
CC -!- FUNCTION: Negative regulator of pollen germination (PubMed:27468890).
CC Prevents pollination in moist environments by inhibiting jasmonic acid
CC synthesis (PubMed:27468890). Stabilizes pollen tube growth
CC (PubMed:27468890). {ECO:0000269|PubMed:27468890}.
CC -!- SUBUNIT: Interacts with TCP4. {ECO:0000269|PubMed:27468890}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:27468890}. Cytoplasm
CC {ECO:0000269|PubMed:27468890}. Note=The nuclear protein is functional.
CC {ECO:0000269|PubMed:27468890}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in pollen (PubMed:27468890).
CC Not detected in roots, stems or leaves (PubMed:27468890).
CC {ECO:0000269|PubMed:27468890}.
CC -!- DEVELOPMENTAL STAGE: Detected first in bicellular pollen and then most
CC abundant in tricellular and mature pollen and growing pollen tubes.
CC {ECO:0000269|PubMed:27468890}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but hyperactive pollen germination.
CC {ECO:0000269|PubMed:27468890}.
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DR EMBL; AC002505; AAC14498.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07844.1; -; Genomic_DNA.
DR EMBL; BT015086; AAT71958.1; -; mRNA.
DR EMBL; BT015900; AAU95436.1; -; mRNA.
DR PIR; T00982; T00982.
DR RefSeq; NP_180217.1; NM_128206.3.
DR AlphaFoldDB; O48716; -.
DR SMR; O48716; -.
DR STRING; 3702.AT2G26490.1; -.
DR iPTMnet; O48716; -.
DR PaxDb; O48716; -.
DR PRIDE; O48716; -.
DR ProteomicsDB; 238985; -.
DR EnsemblPlants; AT2G26490.1; AT2G26490.1; AT2G26490.
DR GeneID; 817190; -.
DR Gramene; AT2G26490.1; AT2G26490.1; AT2G26490.
DR KEGG; ath:AT2G26490; -.
DR Araport; AT2G26490; -.
DR TAIR; locus:2066276; AT2G26490.
DR eggNOG; KOG4155; Eukaryota.
DR HOGENOM; CLU_031007_1_0_1; -.
DR InParanoid; O48716; -.
DR OMA; FWERDKQ; -.
DR OrthoDB; 527415at2759; -.
DR PhylomeDB; O48716; -.
DR PRO; PR:O48716; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O48716; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009846; P:pollen germination; IDA:TAIR.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR045182; JINGUBANG-like.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR22844; PTHR22844; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..465
FT /note="Protein JINGUBANG"
FT /id="PRO_0000438602"
FT REPEAT 94..131
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 132..169
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 204..243
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 246..284
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 293..332
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 336..373
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 378..426
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 13..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 344..346
FT /note="Nuclear exporting signal"
FT /evidence="ECO:0000305|PubMed:27468890"
FT COMPBIAS 18..32
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 344
FT /note="L->A: Increased nuclear localization."
FT /evidence="ECO:0000269|PubMed:27468890"
SQ SEQUENCE 465 AA; 51437 MW; 1311C66726733661 CRC64;
MFTEADSIPR AKYANMMHSD PNLSSTMTQQ TEEEYGIRNS SASAVGTGMY DRMSCEGSPM
MMSPWNQATP FTQTQWSTVE ENLPQNGLIG SLVREEGHIY SLAATKDLLY TGSDSKNIRV
WKNLKEFSAF KCNSGLVKAI VISGEKIFTG HQDGKIRVWK VSPKNQSLHK RSGTLPTLKD
IFKASLKPRN YVEVKKHRTA LWIKHADAVS CLSLNDEQGL LYSASWDRTI KVWRIADSKC
LESIPAHDDA VNSVVSTTEA IVFSGSADGT VKAWKRDQQG KYTKHTLMQT LTKQESAVTA
LAVSKNGAAV YFGSSDGLVN FWEREKQLNY GGILKGHKLA VLCLEVAGSL VFSGSADKTI
CVWKRDGNIH TCLSVLTGHT GPVKCLAVEA DREASERRDK KWIVYSGSLD KSVKVWGVSE
SFIDMNQMSM MQQQQQQHVI SHSEPFMSDG SFSSSAGGAS SHRRH
