ID   JI60_HORVU              Reviewed;         560 AA.
AC   Q00531;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=60 kDa jasmonate-induced protein;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=1511131; DOI=10.1007/bf00040538;
RA   Becker W., Apel K.;
RT   "Isolation and characterization of a cDNA clone encoding a novel jasmonate-
RT   induced protein of barley (Hordeum vulgare L.).";
RL   Plant Mol. Biol. 19:1065-1067(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- INDUCTION: By jasmonate.
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   EMBL; X66376; CAA47017.1; -; mRNA.
DR   PIR; S25092; S25092.
DR   AlphaFoldDB; Q00531; -.
DR   SMR; Q00531; -.
DR   PRIDE; Q00531; -.
DR   ExpressionAtlas; Q00531; baseline.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   PANTHER; PTHR33453; PTHR33453; 2.
DR   Pfam; PF00161; RIP; 1.
DR   SUPFAM; SSF56371; SSF56371; 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Plant defense; Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..560
FT                   /note="60 kDa jasmonate-induced protein"
FT                   /id="PRO_0000221400"
FT   REGION          37..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        201
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   560 AA;  60362 MW;  7FD9BEAA024D4813 CRC64;
     MALDKVAPIV IVTPFNVMTD RYDEFIEKVR KALAGTAGAK VGPKPKSKVE SPVLDKGTFP
     VEQPPRWIHV ELHGKTQGTT TPKPKVAIRS DDAYIMGFTN STGRWFQLSK TGTTYKLVDD
     KAVMAGFDGN YNTLVGGVNN LPTLNLNKFS MAQAAAALWN KASTLSGGIG SDVVDDDDGD
     MLRANDPVKQ AVATLAVAVC EAARFSPVSK VVNAGWIKDK VSVTPDEVNY IKEWGDLSTA
     LLSWMDKQYK DDATIFKKFN GIGITNGEEA LAVVRLVKLV IRSNMAAAPT TDEQLLAYAQ
     LPKHGRYMAE VFAVRIPATA GGDRPAAPSL CTAATAAATS FTARKKNTPR SKPAATARVT
     WSSLGHRLAT SAYGPIVFNL DLHDGNCGQA DEEEDEKNTG RIVCDAIGGD FSNYNKAISE
     TVLTRCGPAE VIYAVLSNGV QGRVDVKLAG LQSRDEVVLV GRIVARSKLF DFGCVLFYNE
     AAGVRVRPGE LVPLARHALA VPLHMPLTIE LDIRHGGSGD EIVRGELEFK TAIDGLHTGR
     LVGVNDAEFE VTILWSEYPW