JJJ2_YEAST
ID JJJ2_YEAST Reviewed; 583 AA.
AC P46997; D6VW25;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=J protein JJJ2;
GN Name=JJJ2; OrderedLocusNames=YJL162C; ORFNames=J0549;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 433 AND 483.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION OF PROBABLE SEQUENCE ERRORS.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP GENE NAME.
RX PubMed=15170475; DOI=10.1038/sj.embor.7400172;
RA Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T.;
RT "The J-protein family: modulating protein assembly, disassembly and
RT translocation.";
RL EMBO Rep. 5:567-571(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 184 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA89457.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; Z49437; CAA89457.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006943; DAA08641.1; -; Genomic_DNA.
DR PIR; S56945; S56945.
DR RefSeq; NP_012373.2; NM_001181595.1.
DR AlphaFoldDB; P46997; -.
DR SMR; P46997; -.
DR BioGRID; 33597; 42.
DR DIP; DIP-4033N; -.
DR IntAct; P46997; 1.
DR MINT; P46997; -.
DR STRING; 4932.YJL162C; -.
DR iPTMnet; P46997; -.
DR MaxQB; P46997; -.
DR PaxDb; P46997; -.
DR PRIDE; P46997; -.
DR EnsemblFungi; YJL162C_mRNA; YJL162C; YJL162C.
DR GeneID; 853277; -.
DR KEGG; sce:YJL162C; -.
DR SGD; S000003698; JJJ2.
DR VEuPathDB; FungiDB:YJL162C; -.
DR eggNOG; KOG0714; Eukaryota.
DR HOGENOM; CLU_490950_0_0_1; -.
DR InParanoid; P46997; -.
DR OMA; ARSKFEC; -.
DR BioCyc; YEAST:G3O-31602-MON; -.
DR PRO; PR:P46997; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P46997; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR GO; GO:0042026; P:protein refolding; IBA:GO_Central.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018253; DnaJ_domain_CS.
DR InterPro; IPR036869; J_dom_sf.
DR Pfam; PF00226; DnaJ; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS00636; DNAJ_1; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 1: Evidence at protein level;
KW Chaperone; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..583
FT /note="J protein JJJ2"
FT /id="PRO_0000071142"
FT DOMAIN 11..79
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT REGION 215..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..259
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..294
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..313
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 229
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 583 AA; 67389 MW; 6CBB8ED37F5F340B CRC64;
MSQVIEPQLD RTTYYSILGL TSNATSSEVH KSYLKLARLL HPDKTKSDKS EELFKAVVHA
HSILTDEDQK LRYDRDLKIK GLHTYQPKKN CHIFKTKAKE SQGASPTLGQ SEAYHRQNKP
YEQQPYGFGV GKKMTSSSKS KVPIFKSFNL KSYQRNHYYS SKKERKHGSP DIDSLFHETN
GASKVRMTDA GKMDTNSQFQ EIWEILGKNA YTHKSYSEDP NSCLGSALSD HEEEEEAGKQ
QQQQQQQQQQ QQHYGMTSKS SSPDEEKKNN KEPKRESRVS PEENGEEETG HKQFKLPKTS
TFSSGSHDSN LQSPFYNHEY RHYARSKFEC KNQFRKSVSP IKEIPATTSA NEGWNILRDI
IEKLNISNVD DRNKDLLFRR DEIGDKNHSD SIDIENLSIK EPKGMKRRKK DDISLEELFQ
SLPREKDYFM MDAINDSLES INLFKKPKTT QSHEQGGTFA QAESNRAKFK PLLEQCGITP
EILDLEIPEI PEFDAVADLE TLKLNVQLFN NQCNKLKETI HQVSLQRLRA DTQFSDMLTQ
KQSIMVWKTY LEFDKSLMDK LNILQERQMQ VIKIFSERCD GKV
