ID   JOSL_ARATH              Reviewed;         360 AA.
AC   O82391;
DT   25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Josephin-like protein;
DE            EC=3.4.19.12;
GN   OrderedLocusNames=At2g29640; ORFNames=T27A16.26;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   3D-STRUCTURE MODELING.
RX   PubMed=12486728; DOI=10.1002/prot.10280;
RA   Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.;
RT   "Structural modeling of ataxin-3 reveals distant homology to adaptins.";
RL   Proteins 50:355-370(2003).
CC   -!- FUNCTION: May act as a deubiquitinating enzyme. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12;
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DR   EMBL; AC005496; AAC35231.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08284.1; -; Genomic_DNA.
DR   PIR; G84698; G84698.
DR   RefSeq; NP_180525.1; NM_128518.2.
DR   AlphaFoldDB; O82391; -.
DR   SMR; O82391; -.
DR   STRING; 3702.AT2G29640.1; -.
DR   MEROPS; C86.A02; -.
DR   PaxDb; O82391; -.
DR   PRIDE; O82391; -.
DR   ProteomicsDB; 232271; -.
DR   EnsemblPlants; AT2G29640.1; AT2G29640.1; AT2G29640.
DR   GeneID; 817514; -.
DR   Gramene; AT2G29640.1; AT2G29640.1; AT2G29640.
DR   KEGG; ath:AT2G29640; -.
DR   Araport; AT2G29640; -.
DR   TAIR; locus:2060704; AT2G29640.
DR   eggNOG; KOG2934; Eukaryota.
DR   HOGENOM; CLU_065889_0_0_1; -.
DR   InParanoid; O82391; -.
DR   OMA; GPYYKNP; -.
DR   OrthoDB; 1482722at2759; -.
DR   PhylomeDB; O82391; -.
DR   PRO; PR:O82391; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O82391; baseline and differential.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IBA:GO_Central.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0016579; P:protein deubiquitination; IBA:GO_Central.
DR   InterPro; IPR040053; JOSD1/2.
DR   InterPro; IPR006155; Josephin.
DR   PANTHER; PTHR13291; PTHR13291; 1.
DR   Pfam; PF02099; Josephin; 1.
DR   SMART; SM01246; Josephin; 1.
DR   PROSITE; PS50957; JOSEPHIN; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Nucleotide-binding; Protease; Reference proteome;
KW   Ubl conjugation pathway.
FT   CHAIN           1..360
FT                   /note="Josephin-like protein"
FT                   /id="PRO_0000053846"
FT   DOMAIN          5..192
FT                   /note="Josephin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00331"
FT   ACT_SITE        18
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00331"
FT   ACT_SITE        129
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00331"
SQ   SEQUENCE   360 AA;  40489 MW;  01701E35AB2C981D CRC64;
     MADSESKIYH ERQRLQFCLL HCLNNLFQDK DAFTKESLNS IAEKLETNDP NKETWTPLSF
     VLKPHHNTIT GNYDVNVMIT ALEGKGKSVV WHDKRIGASS IDLDDADTLM GIVLNVPVKR
     YGGLWRSRHW VVVRKINGVW YNLDSDLVVP QLFRDDDEVR GFLDQNLSLD VIGLIWNLPY
     NANQLPLASN YRVVITHWSD LNGMFSHVTN YPLDFDVYPQ VSSKVSAIYK ATGTKRFNAN
     TRPVTPGKQS SVKGPYYKNP GCTTSCGLRL PRKTECTAAR LIKDLSCKFV MGLRLVVMRK
     KKKKRSPPLK KASSSGISQP SVISVVNDNN HRSAAIEDCI QFINSSSSFT RSNSTCGSKS